LOCUS       sequence-229469-        7154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and 
            mCherry in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Slepak TI, Salay LD, Lemmon VP, Bixby JL
  TITLE     Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal 
            organization, and neuronal morphology.
  JOURNAL   Cytoskeleton (Hoboken). 2012 Jul;69(7):514-27. doi: 
            10.1002/cm.21021. Epub 2012 Mar 7.
  PUBMED    22359282
REFERENCE   2  (bases 1 to 7154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.21021"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2012-07"; volume: 
            "69"; issue: "7"; pages: "514-27"
COMMENT     Sequence Label: pAAV-DYRK3-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..7154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        206..509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        510..713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     664..684
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     710..734
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          862..1240
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             3098..3151
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3179..3886
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3321..3339)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3589..3608
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3901..4377
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4100..4119)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4417..4557
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4417..4546
                     /label=AAV2 ITR
     rep_origin      4632..5087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4719..4738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4929..4950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5104..5123)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5223..5245
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5283..5301)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5369..5473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5474..5542,5543..6334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5692..5711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6505..7093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6994..7013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtctagtt attaatagta atcaattacg gggtcattag
      181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      541 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      601 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      661 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      721 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      781 gggaccgatc cagcctccgc ggattcgaat cccggccggg aacggtgcat tggaacgcgg
      841 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacaaaaaat
      901 gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct
      961 ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa
     1021 cagtgataat ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata
     1081 taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca
     1141 ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct
     1201 tttgctaatc atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt
     1261 ctgtgtgctg gcccatcact ttggcaaaga attgggattc gaacatcgat tgaattcaat
     1321 gggaggcgca gcccgcgatc gcgggaggaa ggacgcggcg ctgccggggg ccgggctccc
     1381 gccgcagcag cggaggttgg gggatggtgt ctatgatact ttcatgatga tagatgaaac
     1441 caagggccca ccctattcgg acacattcag caacccctct gaagcacctg tctccagaag
     1501 gctaaatatt accactgagc cactcacgag aggccacact cagcactttg tgaatggaag
     1561 tgagatgaag gtggaacaac tgtttcaaga atttggcaac agaagatcca atactctcca
     1621 gtcagatggc atcagcaact cggaaaagtc ttctccggct tctcagggga aaagttcaga
     1681 aagcctgagc gcggtgaagt gcaacctttc ctccaggccg tctaaggtgc tgccgctgac
     1741 tcctgagcaa gcgctgaagc agtataaaca ccacctcact gcctacgaga agctggagat
     1801 cgtcagctac ccagaaatct actttgtggg tccgaatgcc aaaaagcggc agggagttat
     1861 tggtggtccc aataacggtg gctacgacga tgcggatggg gcctatattc atgtgccccg
     1921 ggaccatctg gcttaccgct atgaggtgct gaaaatcatc ggcaagggga gttttggaca
     1981 ggtagcccgg gtctatgatc acaaacttcg gcagtacgtg gccctgaaaa tggtgcgcaa
     2041 tgagaaacgc ttccatcgcc aggcagccga ggagatccgg atcttggagc atcttaaaaa
     2101 gcaagacaaa actggtagca tgaacgtcat ccacatgcta gaaagtttca ccttccggaa
     2161 ccacgtgtgc atggcctttg aactgctaag catagacctg tatgagctta ttaaaaaaaa
     2221 caagttccag ggcttcagcg tccagttggt tcggaagttc gcccagtcca tcctacagtc
     2281 cctggacgct ctgcacaaaa acaagatcat ccactgcgac ctgaagccgg aaaacattct
     2341 cctgaaacac cacggccgaa gcgccaccaa ggtcatcgac tttggctcca gctgcttcga
     2401 gtatcagaag ctttacacgt atatccagtc ccgcttctac agagccccag agatcatcct
     2461 ggggtgccgc tacagcaccc cgatcgacat atggagcttt ggttgcatcc ttgcagaact
     2521 tttgacagga cagcccctgt tccctggaga ggacgaagga gaccagttgg cctgcatgat
     2581 agagttgcta ggaatgccac cgcagaaact tctggagcaa tccaagcgtg ccaagtactt
     2641 tattaactcc aaaggcttgc ctcgatactg ctccgtatct acccagacgg acgggagggt
     2701 ggtgcttctc gggggtcgct cacgcagggg taaaaagcga ggcccgccag gcagcaaaga
     2761 ctgggcaacc gcactgaagg gctgtggtga ctacttgttc atagagtttc tgaaacgatg
     2821 cctccagtgg gacccctctg cccgcctcac cccggctcaa gcattaagac atccttggat
     2881 tagcaagtct acacccaaac ctctcaccat ggacaaggtg ccagggaagc gggtagttaa
     2941 ccctacaaat gctttccagg gactgggttc caagctgcct ccagtcgttg ggatagccag
     3001 taagcttaaa gctaacctaa tgtccgaaac cagtggtagt atacctctgt gcagtgtatt
     3061 gccaaagctg attagcctcg accggggtac caccggtgaa ggccgcggca gcctgctgac
     3121 ctgcggcgat gtggaagaaa acccgggccc ggcgccgggc agcactagtc ggaagcttgt
     3181 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3241 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3301 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3361 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3421 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3481 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3541 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3601 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     3661 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     3721 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     3781 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     3841 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaactcg agagatctac
     3901 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     3961 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     4021 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     4081 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     4141 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     4201 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     4261 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     4321 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     4381 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     4441 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     4501 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     4561 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4621 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4681 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
     4741 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4801 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     4861 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4921 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     4981 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     5041 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     5101 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5161 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5221 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5281 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5341 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5401 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5461 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5521 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5581 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5641 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5701 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5761 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5821 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5881 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5941 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6001 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6061 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6121 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6181 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6241 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6301 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6361 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6421 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7141 ttttgctcac atgt
//
