LOCUS       sequence-22947-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M69 pCMV pGL3 Luciferase
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(59..83)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(80..283)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(109..129)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        284..663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtttttggcg tcttccatgg tggctttacc aacagtaccg gaatgccaag cttctagtga
       61 tctgacggtt cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
      121 ccatttgcgt caacggggcg gggttattac gacattttgg aaagtcccgt tgattttggt
      181 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
      241 accgctatcc acgcccattg gtgtactgcc aaaaccgcat caccatggta atagcgatga
      301 ctaatacgta gatgtactgc caagtaggaa agtcccgtaa ggtcatgtac tgggcataat
      361 gccaggcggg ccatttaccg tcattgacgt caataggggg cggacttggc atatgataca
      421 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
      481 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
      541 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
      601 atgggctatg aactaatgac cccgtaattg attactatta ataactagtc aataatcaat
      661 gccaacatgg cggtcatatt ggacatgagc caatataaat gtacatatta tgatatagat
      721 acaacgtatg caatggccaa tagccaatat tgatttatgc tatat
//
