LOCUS       sequence-229470-        7192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and 
            mCherry in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Slepak TI, Salay LD, Lemmon VP, Bixby JL
  TITLE     Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal 
            organization, and neuronal morphology.
  JOURNAL   Cytoskeleton (Hoboken). 2012 Jul;69(7):514-27. doi: 
            10.1002/cm.21021. Epub 2012 Mar 7.
  PUBMED    22359282
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.21021"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2012-07"; volume: 
            "69"; issue: "7"; pages: "514-27"
COMMENT     Sequence Label: pAAV-DYRK2-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..7192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        206..509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        510..713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     664..684
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     710..734
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          862..1240
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             3136..3189
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3217..3924
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3359..3377)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3627..3646
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3939..4415
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4138..4157)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4455..4595
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4455..4584
                     /label=AAV2 ITR
     rep_origin      4670..5125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4757..4776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4967..4988
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5142..5161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5261..5283
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5321..5339)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5407..5511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5512..5580,5581..6372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5730..5749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6543..7131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7032..7051
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtctagtt attaatagta atcaattacg gggtcattag
      181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      541 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      601 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      661 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      721 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      781 gggaccgatc cagcctccgc ggattcgaat cccggccggg aacggtgcat tggaacgcgg
      841 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacaaaaaat
      901 gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct
      961 ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa
     1021 cagtgataat ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata
     1081 taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca
     1141 ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct
     1201 tttgctaatc atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt
     1261 ctgtgtgctg gcccatcact ttggcaaaga attgggattc gaacatcgat tgaattcatg
     1321 ttaaccagga aaccttcggc cgccgctccc gccgcctacc cgaccggccg aggaggggac
     1381 accgccgttc gccagcttca ggcttccccg gggatcggcg cgggggctcc ccgaagcgga
     1441 gtggggaccg gcccgccctc ccccatcgcc ctgccgcctc tccgggccag caacgctacc
     1501 actacagccc acacgattgg cggcagtaag cacaccatga atgaccacct ccacctcaac
     1561 agccacggac agatccaggt tcagcagctg ttcgaggata atagtaacaa aaggacagtg
     1621 ctcacaacac agcccaatgg gcttacaacg gtgggcaaga caggcttgcc tggggtgccc
     1681 gagcggcagc tggagagcat tcaccgacgg cagggcagct ccacctctct gaagtccatg
     1741 gaaggcatgg ggaaggtaaa agcctccccc atgacgcccg aacaagcgat gaagcaatac
     1801 atgcaaaaac tcacagcctt cgaacaccat gagattttta gctaccctga aatatacttc
     1861 ttgggtccaa atgcaaagaa acgccaaggc atgaccggtg gacccaacaa tggtggttat
     1921 gacgacgacc agggatcgta cgtgcaggtg ccccatgatc acgtggctta caggtacgag
     1981 gtcctcaagg tcatcgggaa ggggagcttt gggcaggtgg tcaaggccta tgaccacaaa
     2041 gtccaccagc acgtggccct gaagatggtg cggaacgaga agcgcttcca caggcaggcg
     2101 gccgaggaga tccggatctt ggaacaccta cggaagcagg acaaggacaa cactatgaac
     2161 gtcatccaca tgctggagaa cttcaccttc cgcaaccaca tctgcatgac gttcgagttg
     2221 ctgagcatga acctctatga gctcatcaag aagaataagt tccagggctt cagcctgcct
     2281 ctggtgcgca agtttgccca ctccattctg cagtgcttgg atgctttgca caagaacaga
     2341 ataatccact gtgaccttaa gcccgagaac attttgttaa agcagcaggg tcgaagcagt
     2401 attaaagtga ttgactttgg ctccagttgt tacgagcacc aacgcgtcta cacgtacatc
     2461 cagtcacgct tttaccgggc tccggaagtg atcctcggag ccaggtatgg catgcccata
     2521 gacatgtgga gcctgggttg catcctcgcg gaactcctga cgggttaccc cctcttgcct
     2581 ggggaggatg aaggggacca gctggcttgt atgatcgagc ttttgggcat gccctctcag
     2641 aaactcctgg atgcttccaa acgagccaaa aatttcgtga gctccaaggg ttacccccga
     2701 tactgcacag ttacgactct ttcagatggc tctgtggtcc tcaacggggg ccgatcccgg
     2761 agagggaaac taaggggtcc gccagagagc agagagtggg ggaacgcact gaagggatgc
     2821 gatgaccctc ttttccttga cttcttaaaa cagtgtttgg agtgggatcc cgcggtccgc
     2881 atgaccccgg gccaggcttt gcggcacccg tggcttagga ggcgcttgcc aaagcctccg
     2941 accggagaga agacagcggt gaagagggtc acagagagta ctggtgctat cacctccatt
     3001 tccaagttac ctccaccctc cagttcagct tccaagctga ggactaactt ggcacagatg
     3061 acagatgcca atgggaatat tcagcagagg acagtgttgc cgaaactcgt tagcctcgac
     3121 cggggtacca ccggtgaagg ccgcggcagc ctgctgacct gcggcgatgt ggaagaaaac
     3181 ccgggcccgg cgccgggcag cactagtcgg aagcttgtga gcaagggcga ggaggataac
     3241 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     3301 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     3361 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     3421 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     3481 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     3541 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     3601 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     3661 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     3721 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     3781 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     3841 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     3901 ggcatggacg agctgtacaa gtaactcgag agatctacgg gtggcatccc tgtgacccct
     3961 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     4021 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     4081 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     4141 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     4201 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     4261 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     4321 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     4381 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     4441 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     4501 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     4561 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     4621 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     4681 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4741 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4801 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4861 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     4921 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4981 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     5041 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5101 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     5161 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5221 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5281 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     5341 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5461 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5521 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5581 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5641 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5701 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5761 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5821 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5881 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5941 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6001 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6061 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6121 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6181 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6241 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6301 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6361 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6421 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6481 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6541 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6601 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6661 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6721 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6781 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6841 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6901 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6961 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7021 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7081 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7141 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
