LOCUS       sequence-229474-       11736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression plasmid for FLAG-tagged human T1R2 ECD with 
            signal peptide of influenza hemagglutinin fused to a canonical 
            transmembrane domain from MHC class I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Park J, Selvam B, Sanematsu K, Shigemura N, Shukla D, Procko E
  TITLE     Structural architecture of a dimeric class C GPCR based on 
            co-trafficking of sweet taste receptor subunits.
  JOURNAL   J Biol Chem. 2019 Feb 5. pii: RA118.006173. doi: 
            10.1074/jbc.RA118.006173.
  PUBMED    30723160
REFERENCE   2  (bases 1 to 11736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2019 Feb 5. pii: RA118.006173. doi: 10.1074/jbc.RA118.006173."
COMMENT     Sequence Label: pCEP4-HA-Flag-natT1R2 ECD-MHC
FEATURES             Location/Qualifiers
     source          1..11736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..231
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             274..1311
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1309..1328)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1353..1401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2055..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2635..2659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    4542..4676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4566..4585)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4620..4639
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4819..4841)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5431..7220
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(7522..9447)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGXGGGXGGXGGXGXGGGAGGGGGXGGAGXGGGA
                     GGXGGXGAGGGAGAGGGAGGXGXGGGXGGXGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(9882..9900)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9968..10072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10073..10141,10142..10933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10291..10310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11104..11692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11593..11612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
       61 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
      121 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
      181 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
      241 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
      301 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
      361 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
      421 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
      481 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
      541 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
      601 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
      661 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
      721 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
      781 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
      841 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
      901 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
      961 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     1021 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     1081 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     1141 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     1201 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     1261 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     1321 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     1381 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     1441 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     1501 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     1561 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     1621 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     1681 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     1741 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     1801 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     1861 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     1921 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     1981 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     2041 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     2581 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     2641 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgaagac
     2701 catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc gactacaagg atgacgacga
     2761 cggcagcccc ggaggagctg ctgagaactc ggacttctac ctgcctgggg attacctcct
     2821 gggtggcctc ttctccctcc atgccaacat gaagggcatt gttcacctta acttcctgca
     2881 ggtgcccatg tgcaaggagt atgaagtgaa ggtgataggc tacaacctca tgcaggccat
     2941 gcgctttgcg gtggaggaga tcaacaatga cagcagcctg ctgcctggtg tgctgctggg
     3001 ctatgagatc gtggatgtgt gctacatctc caacaatgtc cagccggtgc tctacttcct
     3061 ggcacacgag gacaacctcc ttcccatcca agaggactac agtaactaca tttcccgtgt
     3121 ggtggctgtc attggccctg acaactccga gtctgtcatg actgtggcca acttcctctc
     3181 cctatttctc cttccacaga tcacctacag cgccatcagc gatgagctgc gagacaaggt
     3241 gcgcttcccg gctttgctgc gtaccacacc cagcgccgac caccacatcg aggccatggt
     3301 gcagcttatg ctgcacttcc gctggaactg gatcattgtg ctggtgagca gcgacaccta
     3361 tggccgcgac aatggccagc tgcttggcga gcgcgtggcc cggcgcgaca tctgcatcgc
     3421 cttccaggag acgctgccca cactgcagcc caaccagaac atgacgtcag aggagcgcca
     3481 gcgcctggtg accattgtgg acaagctgca gcagagcaca gcgcgcgtcg tggtcgtgtt
     3541 ctcgcccgac ctgaccctgt accacttctt caatgaggtg ctgcgccaga acttcactgg
     3601 cgccgtgtgg atcgcctccg agtcctgggc catcgacccg gtcctgcaca acctcacgga
     3661 gctgcgccac ttgggcacct tcctgggcat caccatccag agcgtgccca tcccgggctt
     3721 cagtgagttc cgcgagtggg gcccacaggc tgggccgcca cccctcagca ggaccagcca
     3781 gagctatacc tgcaaccagg agtgcgacaa ctgcctgaac gccaccttgt ccttcaacac
     3841 cattctcagg ctctctgggg agcgtgtcgt ctacagcgtg tactctgcgg tctatgctgt
     3901 ggcccatgcc ctgcacagcc tcctcggctg tgacaaaagc acctgcacca agagggtggt
     3961 ctacccctgg cagctgcttg aggagatctg gaaggtcaac ttcactctcc tggaccacca
     4021 aatcttcttc gacccgcaag gggacgtggc tctgcacttg gagattgtcc agtggcaatg
     4081 ggaccggagc cagaatccct tccagagcgt cgcctcctac taccccctgc agcgacagct
     4141 gaagaacatc caagacatct cctggcacac catcaacaac acgatcccta tgtccatgtg
     4201 ttccaagagg tgccagtcag ggcaaaagaa gaagcctgtg ggcatccacg tctgctgctt
     4261 cgagtgcatc gactgccttc ccggcacctt cctcaaccac actgaagatg aatatgaatg
     4321 ccaggcctgc ccgaataacg agtggtccta ccagagtgag acctcctgct tcaagcggca
     4381 gctggtcttc ctggaatggc atgaggcacc caccatcgct atccccatca tgggtatcgt
     4441 tgctggcctg gttgtccttg cagctgtagt cactggagct gcggtcgctg ctgtgctgtg
     4501 gagaaagaag agctcagatt gactcgaggc cggcaaggcc ggatccagac atgataagat
     4561 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4621 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4681 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     4741 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt
     4801 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     4861 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     4921 tcggggcgca gccatgaggt cgactctaga ggatcgatcc ccgccccgga cgaactaaac
     4981 ctgactacga catctctgcc ccttcttcrc skgrcwrygm mwkywctgcc ccttcttcgc
     5041 ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc
     5101 acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt
     5161 ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca
     5221 gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac
     5281 caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac
     5341 gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat
     5401 agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag
     5461 tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg
     5521 ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag
     5581 ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc
     5641 acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc
     5701 ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt
     5761 atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg
     5821 ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat
     5881 aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg
     5941 gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca
     6001 taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac
     6061 aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag
     6121 cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat
     6181 ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac
     6241 gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt
     6301 ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg
     6361 gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg
     6421 cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc
     6481 ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac
     6541 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc
     6601 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     6661 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     6721 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     6781 ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc
     6841 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa
     6901 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     6961 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
     7021 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     7081 tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt
     7141 agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt
     7201 cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg
     7261 ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg
     7321 agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat
     7381 gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg
     7441 atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag
     7501 ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc
     7561 ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa
     7621 ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt
     7681 aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat
     7741 atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg
     7801 tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg
     7861 tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata
     7921 tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc
     7981 caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc
     8041 acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg
     8101 tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg
     8161 cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc
     8221 tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga
     8281 tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct
     8341 ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact
     8401 acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc
     8461 ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc
     8521 tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     8581 tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc
     8641 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     8701 ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc
     8761 ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcctgc
     8821 ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctccts cccctcctsc
     8881 ccctcctcct sctcctsccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc
     8941 tscccctcct scccctcctg cccctcctcc tsctcctgcc cctcctsccc ctcctcctcc
     9001 tcctgcyccy cctcctsctc ctscccctcc tscccctcct scccctcctc ctsctcctgc
     9061 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc
     9121 tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg ctcctgctcc
     9181 tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac
     9241 accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc
     9301 tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc
     9361 actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg
     9421 tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt
     9481 attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc
     9541 caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct
     9601 ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat
     9661 cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc
     9721 tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca
     9781 gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg
     9841 atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt
     9901 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     9961 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    10021 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    10081 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    10141 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    10201 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    10261 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
    10321 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10381 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10441 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10501 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10561 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
    10621 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    10681 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    10741 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10801 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10861 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10921 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10981 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    11041 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    11101 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    11161 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    11221 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
    11281 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    11341 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    11401 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    11461 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    11521 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    11581 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    11641 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    11701 caacgcggcc tttttacggt tcctggcctt ttgctg
//
