LOCUS       sequence-229478-       10560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector encoding BFP-BSD-Nkx2-5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Yu JSL, Palano G, Lim C, Moggio A, Drowley L, Plowright AT, 
            Bohlooly-Y M, Rosen BS, Hansson EM, Wang QD, Yusa K
  TITLE     CRISPR-KO screen identifies Dmap1 as a regulator of 
            chemically-induced reprogramming and differentiation of cardiac 
            progenitors.
  JOURNAL   Stem Cells. 2019 Apr 1. doi: 10.1002/stem.3012.
  PUBMED    30932271
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2019 Apr 1. doi: 10.1002/stem.3012."
COMMENT     Sequence Label: pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5
FEATURES             Location/Qualifiers
     source          1..10560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..234
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     189..209
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             252..432
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    479..604
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1103..1336
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1521..1565
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1863..1980
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2055..2303
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2055..2075
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2226..2245
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2837..4018
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3068..4010
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3967..3987
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4031..4040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4037..4738
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             4757..4810
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4817..5212
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5231..5284
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6311..6331)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6729..6740)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7192..7209)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7198..7422
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7448..7468)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7448..7466)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7487..7503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7487..7503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7500..7522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7511..7527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7535..7541,7542..7559,7560..7565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7580..7601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7718..7735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7889..8477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7969..7988)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8648..9439,9440..9508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9271..9290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9509..9613)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9639..10094)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9776..9797)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9988..10007
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10221..10243
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10235..10252
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10236..10252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10262..10281
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10262..10280
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
       61 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      121 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      181 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
      241 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
      301 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      361 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      421 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
      481 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
      541 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
      601 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
      661 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
      721 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
      781 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
      841 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
      901 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gcgctgatct
      961 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1021 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1081 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1141 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1201 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1261 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1321 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1381 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     1441 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     1501 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     1561 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     1621 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     1681 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     1741 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     1801 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
     1861 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1921 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1981 cgggtttatt acagggacag cagagatcca gtttggttag taccgggccc tacgcgtcca
     2041 aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca
     2101 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
     2161 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     2221 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     2281 tcttgtggaa aggacgaaac accggagacg catgactcgt ctcagtttaa gagctatgct
     2341 ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
     2401 gagtcggtgc tttttttacg cgtgaggggc gcggaagccg gggcggagga ggagccaacc
     2461 ccgggctggg ctgagacccg cagaggaaga cgctctaggg atttgtcccg gactagcgag
     2521 atggcaaggc tgaggacggg aggctgattg agaggcgaag gtacacccta atctcaatac
     2581 aacctttgga gctaagccag caatggtaga gggaagattc tgcacgtccc ttccaggcgg
     2641 cctccccgtc accacccccc ccaacccgcc ccgaccggag ctgagagtaa ttcatacaaa
     2701 aggactcgcc cctgccttgg ggaatcccag ggaccgtcgt taaactccca ctaacgtaga
     2761 acccagagat cgctgcgttc ccgccccctc acccgcccgc tctcgtcatc actgaggtgg
     2821 agaagagcat gcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     2881 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa agtggcgcgg
     2941 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     3001 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     3061 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     3121 ttgcgtgcct tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc
     3181 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     3241 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     3301 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     3361 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     3421 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     3481 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     3541 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     3601 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     3661 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3721 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3781 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     3841 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     3901 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     3961 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgga
     4021 agcttggcgc gccaccatgg gcagcgagct gattaaggag aacatgcaca tgaagctgta
     4081 catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc
     4141 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     4201 cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca
     4261 gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac
     4321 cacatacgag gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     4381 cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca
     4441 gaagaaaaca ctcggctggg aggccttcac cgagaccctg taccccgctg acggcggcct
     4501 ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat
     4561 caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta
     4621 tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca
     4681 cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatgg
     4741 agggggcagc ggcggggaag gaaggggctc cctgctcacc tgcggcgacg tggaagagaa
     4801 ccccggacct gtcgacatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     4861 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     4921 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     4981 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     5041 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     5101 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     5161 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gcggaggggg
     5221 cagcggcggg gaaggaaggg gctccctgct cacctgcggc gacgtggaag agaaccccgg
     5281 acctatgcat atgttcccca gccctgcgct cacacccacg cctttctcag tcaaagacat
     5341 cctgaacctg gagcagcagc agcgtagcct ggcgtctggg gacctgtctg cgcgcctcga
     5401 ggccaccctg gcccctgcct cctgcatgct ggccgccttc aagcccgagg cctactctgg
     5461 ccccgaggcg gcagcgtccg gcctggcaga gctgcgcgcg gagatgggcc ccgcgccttc
     5521 gccccccaag tgctctcctg ctttcccagc cgcccccaca ttttacccgg gagcctacgg
     5581 tgaccctgac ccagccaaag accctcgggc ggataaaaaa gagctgtgcg cgctgcagaa
     5641 ggcagtggag ctggacaaag ccgagacgga tggcgccgag agaccacgcg cacggcggcg
     5701 acggaagcca cgcgtgctct tctcgcaggc gcaggtctac gagctggagc ggcgcttcaa
     5761 gcaacagcgg tacctgtcgg cgccagagcg cgaccagctg gccagcgtgc tgaagctcac
     5821 gtccacgcag gtcaagatct ggttccagaa ccgtcgctac aagtgcaagc gacagcggca
     5881 ggaccagact ctggagcttc tggggccgcc gccgccgccc gcgcgcagga tcgcggtgcc
     5941 cgtgctggtg cgcgacggga agccctgcct gggggacccc gcggcctacg ctcccgccta
     6001 cggcgtgggt ctcaatgcct atggctacaa cgcctacccc taccccagct acggcggcgc
     6061 ggcctgcagt cccggctaca gctgcgccgc ctaccccgct gcgccccccg ccgcgcagcc
     6121 ccccgccgcc tccgccaaca gcaacttcgt gaactttggc gtcggggact tgaacaccgt
     6181 gcagagtccc gggatgccgc agggcaattc gggcgtctcc acgctgcacg gcatccgagc
     6241 ctggtaggct agcggccgct caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6301 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6361 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6421 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6481 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6541 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6601 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6661 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6721 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6781 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6841 ccccgcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6901 taaaagaaaa ggggggactg gaagggctaa ttcactccca aagaagtcaa gatctgcttt
     6961 ttgcctgtac tgggtctctc tggttagacc agagtctctc tggttagacc agatctgagc
     7021 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7081 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7141 acccttttag tcagtgtgga aaatctctag cagtttaaac ccgctgatca gcctcgactg
     7201 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     7261 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     7321 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     7381 aagtcaatag caggcatgct ggggatgcgg tgggctctat gggcggccgt taattaagct
     7441 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     7501 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccggg agcataaagt
     7561 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7621 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7681 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7741 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7801 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7921 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9601 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     9661 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     9721 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     9781 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     9841 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     9901 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     9961 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
    10021 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
    10081 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10141 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10201 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
    10261 gtaatacgac tcactatagg gcgaattgac tagtacataa cttacggtaa atggcccgcc
    10321 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
    10381 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
    10441 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
    10501 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
//
