LOCUS       sequence-229483-        9979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mCitrine controlled by the CaMV 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid 
            protein CURT1A.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418.
  PUBMED    30884945
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418."
COMMENT     Sequence Label: pN_35S/mCitrine
FEATURES             Location/Qualifiers
     source          1..9979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(445..447,448..450,451..1164)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(490..511)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(751..770)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1098..1119
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1167..1191)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1511..1535
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2866..3495
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3924..4997
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5063..5257
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5493..5515)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5601..5741
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5656..5675
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5756..5773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5927..6515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6007..6026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6761..7552)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8073..8097
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8190..8442)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8465..9037)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(9079..9258)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcatggtg agcaagggcg aggagctgtt caccggggtg
      481 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      541 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      601 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gatgtgcttc
      661 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      721 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      781 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      841 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      901 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      961 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1021 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc
     1081 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1141 ggcatggacg agctgtacaa gtaatcgttc agctttcttg tacaaagtgg tgatatcccg
     1201 cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
     1261 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     1321 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     1381 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag gcatgcgacg
     1441 tcgggcccaa gcttagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
     1501 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     1561 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     1621 gccaaccaca gggttcccct cgggatcaaa gtactttaaa gtactttaaa gtactttaaa
     1681 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     1741 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     1801 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     1861 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     1921 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     1981 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     2041 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     2101 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     2161 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     2221 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     2281 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     2341 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     2401 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     2461 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     2521 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     2581 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     2641 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg
     2701 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     2761 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     2821 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     2881 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     2941 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     3001 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     3061 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     3121 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     3181 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     3241 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     3301 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     3361 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     3421 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     3481 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     3541 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     3601 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     3661 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     3721 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     3781 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     3841 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     3901 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     3961 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     4021 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     4081 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     4141 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     4201 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     4261 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     4321 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     4381 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     4441 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     4501 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     4561 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     4621 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     4681 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     4741 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     4801 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     4861 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     4921 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     4981 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     5041 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     5101 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     5161 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     5221 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     5281 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     5341 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     5401 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     5461 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5521 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5581 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     5641 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     5701 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     5761 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5821 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6361 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6481 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
     6601 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     6661 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta acgcttgagt taagccgcgc
     6721 cgcgaagcgg cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg
     6781 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
     6841 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
     6901 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
     6961 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
     7021 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
     7081 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
     7141 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
     7201 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
     7261 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
     7321 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
     7381 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
     7441 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
     7501 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccccc atgatgttta
     7561 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac
     7621 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa
     7681 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     7741 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg
     7801 aacaggctta tgtccactgg gttcgtgccc gaattgatca caggcagcaa cgctctgtca
     7861 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
     7921 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
     7981 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     8041 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     8101 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
     8161 gaattgaatt atcagcttgc atgccggtcg atctagtaac atagatgaca ccgcgcgcga
     8221 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
     8281 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
     8341 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg
     8401 attcaatctt aagaaacttt attgccaaat gtttgaacga tctgcttgac tctaggggtc
     8461 atcattaggg gcagggcatg ctcatgtaga gcgcctgctc gccgtccgag gcggtgccgt
     8521 cgtacagggc ggtgtccagg ccgcagaggg tgaaccccat ccgccggtac gcgtggatcg
     8581 ccggtgcgtt gacgttggtg acctccagcc agaggtgccc ggcaccccgc tcgcgggcga
     8641 actccgtcgc gagccccatc aacgcgcgcc cgaccccgtg cccccggtgc tccggggcga
     8701 cctcgatgtc ctcgacggtc agccggcggt tccagccgga gtacgagacg accacgaagc
     8761 ccgccaggtc gccgtcgtcc ccgtacgcga cgaacgtccg agagtccggg tcgccgtcct
     8821 ccccgtcgtc cgattcgtcg tccgattcgt cgtcggggaa caccttggtc aggggcgggt
     8881 ccaccggcac ctcccgcagg gtgaagccgt ccccggtggc ggtgacgcgg aagacggtgt
     8941 cggtggtgaa ggacccatcc agtgcctcga tggcctcggc gtcccccggg acactggtgc
     9001 ggtaccggta agccgtgtcg tcaagagtgg tacccatggt agatcccctc gatcgagttg
     9061 agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca
     9121 tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat
     9181 aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg ctcagtggct
     9241 ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc
     9301 gggggtcata acgtgactcc cttaattctc cgctcatgat cgataattcg agctctccca
     9361 tatggtcgac tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc
     9421 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
     9481 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
     9541 gagaggccta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat ctccaggaga
     9601 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
     9661 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
     9721 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
     9781 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
     9841 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
     9901 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     9961 cagaagacca aagggctat
//
