LOCUS       sequence-229487-       10434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Tet-on 3G from the EF1Alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, 
            Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
  TITLE     CRISPR Activation Screens Systematically Identify Factors that Drive
            Neuronal Fate and Reprogramming.
  JOURNAL   Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003.
  PUBMED    30318302
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003."
COMMENT     Sequence Label: pHR-EF1Alpha-Tet-on 3G
FEATURES             Location/Qualifiers
     source          1..10434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             60..693
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    740..865
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1362..1595
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1780..1824
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2109..2226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          2454..3376
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3333..3353
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3472..4215
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             4225..4281
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    4357..4945
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4410..4430)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4828..4839)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4948..4964)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4949..4965)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5055..5288
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5313..5331)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5314..5331)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5621..5640)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5854..5873)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5973..5995
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6033..6051)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6119..6223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6224..6292,6293..7084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6442..6461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7255..7843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7744..7763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7997..8014
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8084..8104)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8089..8418
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8269..8404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9552..9617
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9747..9767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10192..10326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10229..10248)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10283..10302
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
       61 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
      121 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      181 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      241 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      301 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      361 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      421 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      481 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      541 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      601 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      661 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      721 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      781 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      841 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      901 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      961 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     1021 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1081 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1141 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1201 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1261 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1321 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1381 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1441 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1501 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1561 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1621 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1681 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1741 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1801 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1861 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1921 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1981 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2041 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     2101 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2161 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2221 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     2281 gaattcctgc agccaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc
     2581 ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     2641 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2701 agccatttaa aatttttgat gacgtgctgc gacgcttttt ttctggcaag atagtcttgt
     2761 aaatgcgggc caggatctgc acactggtat ttcggttttt gggcccgcgg ccggcgacgg
     2821 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     2881 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     2941 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3001 aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg
     3061 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3121 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt
     3181 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3241 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3301 tggccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3361 ttttcttcca tttcaggtgt cgtgaacagg ggatctggag ctctcgagaa ttctcacgcg
     3421 tctgcaggat atcaagcttg cggtaccgcg ggcccgggat ccgccgccac catgtctaga
     3481 ctggacaaga gcaaagtcat aaactctgct ctggaattac tcaatggagt cggtatcgaa
     3541 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     3601 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     3661 catacccact cctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     3721 aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     3781 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     3841 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     3901 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     3961 gattctatgc ccccacttct gaaacaagca attgagctgt tcgaccggca gggagccgaa
     4021 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     4081 gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat
     4141 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     4201 gacatgctcc ccgggggaag cggagctact aacttcagcc tgctgaagca ggctggagac
     4261 gtggaggaga accctggacc tgcaccagga agtgcggccg cataagactc tagagtcgac
     4321 ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     4381 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4441 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4501 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4561 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4621 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4681 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4741 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4801 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4861 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4921 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa ttaattcgag
     4981 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5041 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     5101 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5161 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5221 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5281 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     5341 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     5401 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     5461 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     5521 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     5581 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     5641 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     5701 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     5761 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     5821 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5881 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5941 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6001 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     6061 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6241 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6301 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6361 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6421 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6481 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6541 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6601 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6661 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6721 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6781 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6841 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6901 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6961 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7021 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7081 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7141 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7201 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7381 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7621 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7861 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7921 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7981 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8041 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
     8101 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8161 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8221 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8281 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8341 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8401 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
     8461 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     8521 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     8581 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     8641 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     8701 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt
     8761 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     8821 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
     8881 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
     8941 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     9001 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     9061 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
     9121 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     9181 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     9241 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
     9301 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga
     9361 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
     9421 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct
     9481 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
     9541 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
     9601 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
     9661 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
     9721 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
     9781 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
     9841 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
     9901 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
     9961 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
    10021 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
    10081 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
    10141 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    10201 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10261 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10321 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    10381 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctct
//
