LOCUS       sequence-229489-        8787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Mecom isoform 2 from the TRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, 
            Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
  TITLE     CRISPR Activation Screens Systematically Identify Factors that Drive
            Neuronal Fate and Reprogramming.
  JOURNAL   Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003.
  PUBMED    30318302
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003."
COMMENT     Sequence Label: TetO-FUW-Mecom 2
FEATURES             Location/Qualifiers
     source          1..8787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        150..529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        531..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             747..927
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    974..1099
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1592..1825
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2010..2054
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2352..2469
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2526..2544,2545..2567,2568..2586,2587..2609,2610..2628,
                     2629..2651,2652..2670,2671..2693,2694..2712,2713..2735,
                     2736..2754,2755..2777,2778..2796)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2829..2867
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2864..2888
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3391..3979
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3444..3464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3862..3873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3982..3998)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3983..3999)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4504..4684
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4710..4727)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4716..4940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4986..5414
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5073..5092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5283..5304
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5423..5443)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5428..5757
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5608..5743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5670..5689
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5805..5852
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5871..6245
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6375..6496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6412..6431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6466..6485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6545..6561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6545..6561)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6558..6580)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6569..6585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6593..6599,6600..6617,6618..6623))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6638..6659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6776..6793)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6947..7535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7027..7046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7706..8497,8498..8566))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8329..8348
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8567..8671)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8746..8765)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
       61 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      121 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      541 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      601 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      661 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      721 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
      781 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      841 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      901 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      961 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     1021 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     1081 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     1141 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1201 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1261 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1321 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1381 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1441 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1501 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1561 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1621 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1681 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1741 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1801 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1861 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1921 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1981 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2041 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2101 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2161 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2221 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2281 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     2341 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2401 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2461 caaaattttc gggtttatta cagggacagc agagatccag tttggttaga tctcgagttt
     2521 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2581 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc
     2821 cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
     2881 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     2941 gatccagcct ccgcggcccc gaattcgcta gccaccatga gatccaaagg cagggcaagg
     3001 aaactggcca caagtaatga gtgtgcctat ggcaactatc ctgaaatacc tttggaagaa
     3061 atgccagatg ctgatgcaga tgggataacc agtgttccct ccctccacat tcaagagcca
     3121 tgctctcctg cgacgtccag tgagtcattt actcctaagg agggctcgcc atacaaagct
     3181 cccatctaca tccctgatga catccctatt cctgatgagt ttgagcttcg agagtcaact
     3241 atgcctggag caggacttgg aatatggacc aaaaggaaga ttgaaatagg cgaaaagttt
     3301 gggccataca tgggagagca gagatcagac ctgaaagatt ccagctatgg atgggagaac
     3361 aggtaggaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     3421 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3481 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3541 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3601 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3661 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3721 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3781 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3841 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3901 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3961 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     4021 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     4081 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     4141 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     4201 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     4261 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     4321 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     4381 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     4441 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     4501 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4561 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4621 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     4681 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4741 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4801 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4861 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4921 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     4981 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5041 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5101 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5161 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5221 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5281 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5341 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5401 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5461 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5521 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5581 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5641 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     5701 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5761 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     5821 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     5881 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     5941 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     6001 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     6061 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     6121 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     6181 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     6241 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     6301 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     6361 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6421 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6481 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6541 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6601 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6661 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6721 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6781 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6841 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6901 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6961 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7021 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7081 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7141 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7201 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7261 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7321 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7381 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7441 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7501 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7561 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7621 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7681 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7741 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7801 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7861 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7921 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7981 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8041 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8101 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8161 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8221 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8281 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8341 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8401 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8461 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8521 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8581 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8641 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8701 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     8761 tgccgcatag ttaagccagt atctgct
//
