LOCUS       sequence-229490-       10554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Mecom isoform 1 from the TRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, 
            Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
  TITLE     CRISPR Activation Screens Systematically Identify Factors that Drive
            Neuronal Fate and Reprogramming.
  JOURNAL   Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003.
  PUBMED    30318302
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003."
COMMENT     Sequence Label: TetO-FUW-Mecom 1
FEATURES             Location/Qualifiers
     source          1..10554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        146..525
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        527..725
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             743..923
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    970..1095
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1588..1821
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2006..2050
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2348..2465
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2522..2540,2541..2563,2564..2582,2583..2605,2606..2624,
                     2625..2647,2648..2666,2667..2689,2690..2708,2709..2731,
                     2732..2750,2751..2773,2774..2792)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2825..2863
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2860..2884
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5154..5742
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5207..5227)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5625..5636)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5745..5761)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5746..5762)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6267..6447
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6473..6490)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6479..6703
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6749..7177
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6836..6855)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7046..7067
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7186..7206)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7191..7520
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7371..7506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7433..7452
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7568..7615
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7634..8008
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8138..8259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8175..8194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8229..8248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8308..8324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8308..8324)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8321..8343)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8332..8348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8356..8362,8363..8380,8381..8386))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8401..8422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8539..8556)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8710..9298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8790..8809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9469..10260,10261..10329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10092..10111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10330..10434)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10509..10528)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
       61 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
      121 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      781 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      841 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      901 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      961 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     1021 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1081 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1141 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1201 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1261 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1321 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1381 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1441 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1501 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1561 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1621 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1681 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1741 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1801 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1861 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1921 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1981 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     2041 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2101 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2161 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2221 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2281 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     2341 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2401 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2461 attttcgggt ttattacagg gacagcagag atccagtttg gttagatctc gagtttacca
     2521 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2581 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     2641 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     2701 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2761 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg
     2821 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     2881 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     2941 cagcctccgc ggccccgaat tcgctagcca ccatgaagag tgaagaggac ccgcacgaac
     3001 ccatggcgcc tgacatccac gaagaacggc agcaccgctg tgaggactgt gaccagctct
     3061 ttgaatccaa ggcagagcta gccgatcacc agaagttccc atgcagcaca cctcactcgg
     3121 ccttctccat ggtggaggag gacttgcaac aaaacctgga gagtgagagc gatctccgag
     3181 agatccatgg caaccaggac tgtaaggaat gtgaccgagt tttccccgat ctgcaaagct
     3241 tggagaagca catgctgtca catactgagg agagggaata caagtgtgat cagtgtccca
     3301 aggcatttaa ctggaagtcc aatttaattc gccaccagat gtcacatgac agtggaaagc
     3361 actatgagtg tgaaaactgt gccaaggttt tcacggaccc tagcaacctt cagcgacaca
     3421 ttcgatctca gcatgttggt gcccgggctc atgcttgccc cgagtgtggt aaaacatttg
     3481 ccacttcgtc aggcctcaaa cagcacaagc acatccacag cagtgtgaag ccctttatct
     3541 cattctctca atcaatgtac ccatttcctg atagagactt gaggtcgtta cctttgaaaa
     3601 tggagcccca atcaccaagt gaagttaaga aactgcagaa gggaagctct gagtcccctt
     3661 ttgacctcac cactaagaga aaggatgaga agcccttgac ttcaggcccc tcgaagcctt
     3721 caggaacacc agccacaagc caagaccagc ccctggatct aagtatgggc agtaggggta
     3781 gagccagtgg gacaaagttg actgagcctc gaaaaaacca tgtgtttggg gaaaagaaag
     3841 gaagcaacat ggatactagg ccatcttcag atggctcctt gcagcatgcc agacccactc
     3901 ccttcttcat ggaccccatt tatagagtag agaaaagaaa gttaactgac ccgcttgaag
     3961 ctttgaaaga aaaatacttg agaccttctc caggattctt gtttcacccg caaatgtcag
     4021 caattgagaa catggcagaa aagctggaaa gcttcagcgc cctcaaacct gaggccagcg
     4081 agctcctgca gtccgtgccc tccatgttca gcttccgagc tcctcccaac accctgccag
     4141 agaacctgct gcggaagggg aaagagcgct acacctgcag gtactgtggc aagatatttc
     4201 caaggtctgc gaacctaaca cggcacttga gaacccacac aggagagcaa ccttacagat
     4261 gcaaatactg tgatagatca ttcagcattt cttccaacct gcagcgacat gtgcgcaaca
     4321 tccacaacaa ggagaagcca tttaagtgtc atttatgtga cagatgtttt ggtcaacaaa
     4381 ccaatcttga cagacacctg aagaaacatg agaacggcaa catgtctggg acggcaacgt
     4441 cctcgcctca ctcagagcta gaaagcgcag gcgcaatcct ggatgacaaa gaagatgcgt
     4501 actttacaga gatccgcaat ttcatcggga acagcaacca tggtagccag tctcctcgga
     4561 acatggaaga gaggatgaat ggcagtcact tcaaggataa aaaggctttg gcaaccagcc
     4621 aaaattcaga tttattggat gatgaagaag tagaagatga ggtgttgttg gatgaggagg
     4681 atgaagacaa tgatattcct ggaaagccca gaaaggagct aggggtgact cgtttagacg
     4741 aggagatccc ggaggatgac tacgaagaag ctggtgccct ggagatgagc tgtaaggcgt
     4801 ccccggtgag gtataaagag gaagactata aatctggcct ttctgctcta gatcacataa
     4861 ggcacttcac agatagcctc aaaatgaggg aaatggaaga gaatcaatac actgacgctg
     4921 agctgtcctc cattagttct tctcatgtgc cagaggagct taaacagacg ttacacagaa
     4981 agtccaaatc acaggcatat gctatgatgt tgtcactgtc tgacaaggat tccctccatc
     5041 ccacctccca cagttcctcc aacgtgtggc acagcatggc aagggctgca gcagaatcca
     5101 gtgccatcca gtccataagc catgtatagg aattcgatat caagcttatc gataatcaac
     5161 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5221 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5281 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5341 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5401 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5461 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5521 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     5581 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5641 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     5701 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     5761 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     5821 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     5881 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5941 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     6001 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     6061 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     6121 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     6181 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     6241 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6301 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6361 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6421 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     6481 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6541 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6601 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6661 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     6721 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     6781 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6841 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6901 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6961 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7021 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7081 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7141 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     7201 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7261 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7321 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7381 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7441 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7501 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     7561 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     7621 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     7681 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     7741 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     7801 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     7861 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     7921 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     7981 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     8041 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8101 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8161 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8221 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     8281 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8341 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8401 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8461 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8521 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8581 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8641 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8701 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8761 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8821 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8881 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8941 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9001 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9061 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9121 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9181 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9241 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9301 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9361 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9421 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9481 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9541 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9601 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9661 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9721 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9781 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9841 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9901 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9961 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10021 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10081 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10141 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10201 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10261 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10321 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10381 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10441 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    10501 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccct
//
