LOCUS       sequence-229492-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chloroplast-targeted expression of CURT_fluoA controlled by the CaMV
            35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid 
            protein CURT1A.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418.
  PUBMED    30884945
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418."
COMMENT     Sequence Label: pN_35S/CTP-fluoA
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(610..612,613..1323)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(652..673)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(913..932)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1260..1281
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1650..1674)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1994..2018
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3349..3978
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4407..5480
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5546..5740
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5976..5998)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6084..6224
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6139..6158
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6239..6256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6410..6998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6490..6509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7244..8035)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8556..8580
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8673..8925)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8948..9520)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(9562..9741)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcatggct tcctctatgc tctcttccgc tactatggtt
      481 gcctctccgg ctcaggccac tatggtcgct cctttcaacg gacttaagtc ctccgctgcc
      541 ttcccagcca cccgcaaggc taacaacgac attacttcca tcacaagcaa cggcggaaga
      601 gttaactgcg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      661 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      721 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      781 cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac
      841 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      901 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      961 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1021 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1081 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1141 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1201 aaccactacc tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     1261 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1321 aagggaggtg gtggatctgc ttcttcagaa gagacctcgt ccattgatac caacgaactc
     1381 atcacagatt tgaaggaaaa gtgggatggt cttgagaaca aatctactgt acttatatat
     1441 ggaggaggag ccattgttgc tgtttggtta tcttccattg ttgttggtgc catcaactct
     1501 gttcctctgc ttccgaaagt tatggaactt gtcggactag ggtacactgg atggtttgtc
     1561 tacagatacc ttctcttcaa gtcaagcaga aaggaattgg ctgaggatat tgaatccttg
     1621 aagaagaaga tcgcaggaag cgaatagtcg ttcagctttc ttgtacaaag tggtgatatc
     1681 ccgcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc
     1741 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
     1801 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     1861 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgcg
     1921 acgtcgggcc caagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     1981 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2041 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     2101 catgccaacc acagggttcc cctcgggatc aaagtacttt aaagtacttt aaagtacttt
     2161 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     2221 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     2281 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     2341 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     2401 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     2461 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     2521 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     2581 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     2641 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     2701 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     2761 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     2821 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     2881 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     2941 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     3001 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     3061 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     3121 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     3181 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     3241 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     3301 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     3361 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     3421 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     3481 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     3541 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     3601 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     3661 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     3721 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     3781 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     3841 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     3901 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     3961 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     4021 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     4081 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     4141 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     4201 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     4261 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     4321 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
     4381 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     4441 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     4501 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     4561 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     4621 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     4681 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     4741 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     4801 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     4861 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     4921 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     4981 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     5041 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     5101 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     5161 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     5221 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     5281 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     5341 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     5401 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     5461 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     5521 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     5581 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     5641 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     5701 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     5761 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     5821 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     5881 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     5941 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     6001 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     6061 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     6121 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     6181 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     6241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6841 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcatgata
     7081 tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa gcagacttga
     7141 cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg agttaagccg
     7201 cgccgcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct
     7261 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     7321 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     7381 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     7441 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     7501 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     7561 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     7621 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     7681 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     7741 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     7801 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     7861 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     7921 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     7981 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt
     8041 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
     8101 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
     8161 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     8221 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa
     8281 ccgaacaggc ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg
     8341 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
     8401 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
     8461 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
     8521 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     8581 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
     8641 gccgaattga attatcagct tgcatgccgg tcgatctagt aacatagatg acaccgcgcg
     8701 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
     8761 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
     8821 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
     8881 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctaggg
     8941 gtcatcatta ggggcagggc atgctcatgt agagcgcctg ctcgccgtcc gaggcggtgc
     9001 cgtcgtacag ggcggtgtcc aggccgcaga gggtgaaccc catccgccgg tacgcgtgga
     9061 tcgccggtgc gttgacgttg gtgacctcca gccagaggtg cccggcaccc cgctcgcggg
     9121 cgaactccgt cgcgagcccc atcaacgcgc gcccgacccc gtgcccccgg tgctccgggg
     9181 cgacctcgat gtcctcgacg gtcagccggc ggttccagcc ggagtacgag acgaccacga
     9241 agcccgccag gtcgccgtcg tccccgtacg cgacgaacgt ccgagagtcc gggtcgccgt
     9301 cctccccgtc gtccgattcg tcgtccgatt cgtcgtcggg gaacaccttg gtcaggggcg
     9361 ggtccaccgg cacctcccgc agggtgaagc cgtccccggt ggcggtgacg cggaagacgg
     9421 tgtcggtggt gaaggaccca tccagtgcct cgatggcctc ggcgtccccc gggacactgg
     9481 tgcggtaccg gtaagccgtg tcgtcaagag tggtacccat ggtagatccc ctcgatcgag
     9541 ttgagagtga atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga
     9601 gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc
     9661 aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctcagtg
     9721 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc
     9781 ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcgataat tcgagctctc
     9841 ccatatggtc gactagagcc aagctgatct cctttgcccc ggagatcacc atggacgact
     9901 ttctctatct ctacgatcta ggaagaaagt tcgacggaga aggtgacgat accatgttca
     9961 ccaccgataa tgagaagatt agcctcttca atttcagaaa gaatgctgac ccacagatgg
    10021 ttagagaggc ctacgcggca ggtctcatca agacgatcta cccgagtaat aatctccagg
    10081 agatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
    10141 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
    10201 ttcaaggctt gcttcataaa ccaaggcaag taatagagat tggagtctct aagaaagtag
    10261 ttcctactga atcaaaggcc atggagtcaa aaattcagat cgaggatcta acagaactcg
    10321 ccgtgaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa
    10381 tcttcgtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
    10441 tctcagaaga ccaaagggct at
//
