LOCUS       sequence-22951-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M57 pPET28a-TEV-full-length human beta catenin
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..80
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             84..116
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             126..146
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             192..227
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 taactttaag aaggagatat accatgggca gcagccatca tcatcatcat cacagcagcg
       61 gcctggtgcc gcgcggcagc catatggcta gcatgactgg tggacagcaa atgggtcgcg
      121 gatccgaaaa cctgtatttt cagggcatgg ctactcaagc tgatttgatg gagttggaca
      181 tggccatgga accagacaga aaagcggctg ttagtcactg gcagcaacag tcttacctgg
      241 actctggaat ccattctggt gccactacca cagctccttc tctgagtggt aaaggcaatc
      301 ctgaggaaga ggatgtggat acctcccaag tcctgtatga gtgggaacag ggattttctc
      361 agtccttcac tcaagaacaa gtagctgata ttgatggaca gtatgcaatg actcgagctc
      421 agagggtacg agctgctatg ttccctgaga cattagatga gggcatgcag atcccatcta
      481 cacagtttga tgctgctcat cccactaatg tccagcgttt ggctgaacca tcacagatgc
      541 tgaaacatgc agttgtaaac ttgattaact atcaagatga tgcagaactt gccacacgtg
      601 caatccctga actgacaaaa ctgctaaatg acgaggacca ggtggtggtt aataaggctg
      661 cagttatggt ccatcagctt tctaaaaagg aagcttccag acacgctatc atgcgttctc
      721 ctcagatggt gtctgctatt gtacgtacca tgcagaatac aaatgatgta gaaacagctc
      781 gttgtaccgc tgggaccttg cataaccttt cccatcatcg tgagggctta ctggccatct
      841 ttaa
//
