LOCUS       sequence-22952-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M58 pPET28b-TEV-full-length zebrafish beta cat...
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             91..123
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             151..171
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             217..252
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg
      121 ggtcgggatc cgaattcgag ctccgtcgac gaaaacctgt attttcaggg catggctacc
      181 cagtctgact tgatggagct ggaaatggcc atggatccgg atcgcaaggc tgcagtcagc
      241 cattggcagc aacagtctta cctggactca ggaatacact ctggggccac aactactgcc
      301 ccgtccctga gtggcaaagg caacccggag gatgacgatg tggataatca ggtgctttat
      361 gagtgggagc agggcttcaa ccagtccttc aaccaagagc aagtagcaga catcgatggt
      421 caatacgcca tgaccagagc ccagagagtc cgggcggcca tgttcccgga gactctcgat
      481 gagggcatgc agataccttc cacacagttc gactctgcgc atcccaccaa tgtccagcgc
      541 ctggctgagc cctcgcagat gctcaaacat gccgtggtca acctcatcaa ctaccaggac
      601 gacgcagagc tggccactcg tgccattcca gagctcacca aactactgaa cgacgaggat
      661 caggtggtgg ttaataaggc agctgtgatg gtgcaccagc tctccaagaa agaggcctct
      721 cgtcacgcca tcatgcgctc cccacagatg gtatcggcca ttgtgaggac catgcagaac
      781 actaatgatg tagaaacagc ccgttgcacc tctggcaccc tacacaatct ttcccaccac
      841 agagaangac tgcttgccat ctttaaatca ggaggcatcc ctgccctcgt caaaatgctc
      901 ggctcccctg tggactctgt gctgttttac gctattacaa ctctacacaa cctgctactg
      961 caccaagaag gagccaaga
//
