LOCUS       sequence-229523-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For production of lentivirus to express luciferase, primarily for in
            vivo bioluminescence tracking experiments..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pRRL Luciferase
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..127
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      199..208
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(271..289)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
       61 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
      121 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
      181 ctctccccag ggggatccgc caccatggaa gacgccaaaa acataaagaa aggcccggcg
      241 ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
      301 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact
      361 tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
      421 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
      481 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
      541 gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg
      601 ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg
      661 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
      721 cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt
      781 gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat
      841 a
//
