LOCUS       sequence-22953-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M59 pPET28a-TEV-human beta cat domains R1-C
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             91..123
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg
      121 ggtcgcggat ccgaaaacct gtattttcag ggcaacttga ttaactatca agatgatgca
      181 gaacttgcca cacgtgcaat ccctgaactg acaaaactgc taaatgacga ggaccaggtg
      241 gtggttaata aggctgcagt tatggtccat cagctttcta aaaaggaagc ttccagacac
      301 gctatcatgc gttctcctca gatggtgtct gctattgtac gtaccatgca gaatacaaat
      361 gatgtagaaa cagctcgttg taccgctggg accttgcata acctttccca tcatcgtgag
      421 ggcttactgg ccatctttaa gtctggaggc attcctgccc tggtgaaaat gcttggttca
      481 ccagtggatt ctgtgttgtt ttatgccatt acaactctcc acaacctttt attacaccaa
      541 gaaggagcta aaatggcagt gcgtttagct ggtgggctgc agaaaatggt tgccttgctc
      601 aacaaaacaa atgttaaatt cttggctatt acgacagact gccttcaaat tttagcttat
      661 ggcaaccaag aaagcaagct catcatactg gctagtggtg gaccccaagc tttagtaaat
      721 ataatgagga cctatactta cgaaaaacta ctgtggacca caagcagagt gctgaaggtg
      781 ctatctgtct gctctagtaa taagccggct attgtagaag ctggtggaat gcaagcttta
      841 ggacttcacc tgacagatcc aagtcaacgt cttgttcaga actgtctttg gactctcagg
      901 aatctttcan atgctgcaac taaacaggaa gggatggaag gtctccttgg gactcttgtt
      961 cagcttctgg gttc
//
