LOCUS       sequence-229532-        4557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Pastan I, Gottesman MM, Ueda K, Lovelace E, Rutherford AV, 
            Willingham MC
  TITLE     A retrovirus carrying an MDR1 cDNA confers multidrug resistance and 
            polarized expression of P-glycoprotein in MDCK cells.
  JOURNAL   Proc Natl Acad Sci U S A. 1988 Jun . 85(12):4486-90.
  PUBMED    2898143
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1988 Jun . 85(12):4486-90."
COMMENT     Sequence Label: pHaMDRwt
FEATURES             Location/Qualifiers
     source          1..4557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(337..356)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             1814..2005
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1999..2021)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2107..2247
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2162..2181
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2262..2279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2433..3021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2513..2532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3192..3983,3984..4052))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3815..3834
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4053..4157)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4225..4243
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctctaagtg attgactacc cgttagtggg ggtctttcat ttgggggctc gtccgggatc
       61 gggagacccc tgcccaggga ccaccgaccc accaccggga ggcaagccgg ccggcgtttg
      121 tcttgtctgt tgtgtcttgt cctgtgaacg atcgatcaat aggctcagat ctggggacta
      181 tctgggcggg ccagagaagg agctgacgag ctcggacttc tcccccgcag ccctggaaga
      241 cgttccaagg gtggttggag gagagggaga tccggatcct ctacgccgga cgcatcgtgg
      301 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
      361 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
      421 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
      481 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
      541 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
      601 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
      661 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
      721 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
      781 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
      841 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
      901 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
      961 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     1021 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     1081 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     1141 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     1201 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca
     1261 aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg
     1321 catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
     1381 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
     1441 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
     1501 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat
     1561 gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt
     1621 aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc
     1681 cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat
     1741 cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta
     1801 cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag
     1861 aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga
     1921 catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt
     1981 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     2041 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     2101 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     2161 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     2221 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     2281 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2341 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2401 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2521 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2641 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2701 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2761 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2821 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2881 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2941 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3001 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3061 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3481 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     3541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3781 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     3841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4201 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     4261 ttaattctca tgtttgacag cttatcatcg ataagctttc ctcaccaaac gagagacggc
     4321 ctctacaatc atcaagaaga tactggaaga aatcttcctc ttggaatgcc caaggtaatc
     4381 tggtcagaca acggccctac tttcgttgcc aaggtaagcc agggtgtggc caagtattta
     4441 gaggtcgatt gaaaattaca ttgtatttac agacctcaaa gttcaggaca ggtagggtaa
     4501 ataaataaaa ctctaaaaga gaccctgacc aaattaacca tggagactgg cacagac
//
