LOCUS       sequence-22955-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M63 pPET28a-TEV-human beta cat domain C(666-781)
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             134..154
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             533..550
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(603..621)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      617..664
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(788..807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatggctagc atgactggtg gacagcaaat
      121 gggtcgcgga tccgaaaacc tgtattttca gggcaagcca caagattaca agaaacggct
      181 ttcagttgag ctgaccagct ctctcttcag aacagagcca atggcttgga atgagactgc
      241 tgatcttgga cttgatattg gtgcccaggg agaacccctt ggatatcgcc aggatgatcc
      301 tagctatcgt tcttttcact ctggtggata tggccaggat gccttgggta tggaccccat
      361 gatggaacat gagatgggtg gccaccaccc tggtgctgac tatccagttg atgggctgcc
      421 agatctgggg catgcccagg acctcatgga tgggctgcct ccaggtgaca gcaatcagct
      481 ggcctggttt gatactgacc tttaactcga caagcttgcg gccgcactcg agcaccacca
      541 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      601 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      661 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      721 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      781 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      841 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      901 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggnt
      961 tttcgccctt tga
//
