LOCUS       sequence-22956-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M64 pGEX4T-1-TEV-human beta cat domain N(1-137)
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..118
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     primer_bind     complement(517..539)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(684..702)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tggttccgcg tggatccgaa acctgtattt tcagggcatg gctactcaag ctgatttgat
       61 ggagttggac atggccatgg aaccagacag aaaagcggct gttagtcact ggcagcaaca
      121 gtcttacctg gactctggaa tccattctgg tgccactacc acagctcctt ctctgagtgg
      181 taaaggcaat cctgaggaag aggatgtgga tacctcccaa gtcctgtatg agtgggaaca
      241 gggattttct cagtccttca ctcaagaaca agtagctgat attgatggac agtatgcaat
      301 gactcgagct cagagggtac gagctgctat gttccctgag acattagatg agggcatgca
      361 gatcccatct acacagtttg atgctgctca tcccactaat gtccagcgtt tggctgaacc
      421 atcacagatg ctgaaacatg cagttgtata agtcgagcgg ccgcatcgtg actgactgac
      481 gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
      541 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
      601 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
      661 gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      721 gataataatg gtttcttaga cgtc
//
