LOCUS       sequence-229567-       10567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of GFP-Kif26b-C in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Karuna EP, Choi SS, Scales MK, Hum J, Cohen M, Fierro FA, Ho HH
  TITLE     Identification of a WNT5A-Responsive Degradation Domain in the 
            Kinesin Superfamily Protein KIF26B.
  JOURNAL   Genes (Basel). 2018 Apr 5;9(4). pii: genes9040196. doi: 
            10.3390/genes9040196.
  PUBMED    29621187
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2018-04-5"; volume: "9(4). pii: genes9040196. 
            doi"; pages: " 10.3390/genes9040196"
COMMENT     Sequence Label: pLEX307 GFP-Kif26b-C
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1947..2157
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1969..2165
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2016..2151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2197..2799
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2197..2216)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2693..2713
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2842..2959
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3097..4275
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3328..4266
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4223..4243
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4295..4319
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4347..4349,4350..4352,4353..5063)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4347..4349,4350..4352,4353..5063)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4392..4413)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4653..4672)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5000..5021
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6253..6387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6477..6501)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6509..6550
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6601..7189
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6654..6674)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7072..7083)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7261..7494
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7572..7693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7733..7868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7889..7907)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7917..7934)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7917..7933)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7926..7948)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8075..8530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8162..8181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8372..8393
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8556..8660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8661..8729,8730..9521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8879..8898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9692..10280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10181..10200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10434..10451
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca
     1921 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt
     1981 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     2041 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     2101 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     2161 gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg
     2221 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     2281 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     2341 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     2401 gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     2461 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     2521 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     2581 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     2641 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     2701 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     2761 cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca
     2821 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2881 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2941 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg
     3001 gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc
     3061 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
     3121 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     3181 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     3241 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     3301 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     3361 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     3421 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     3481 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     3541 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     3601 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     3661 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     3721 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     3781 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     3841 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     3901 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     3961 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     4021 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     4081 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     4141 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     4201 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     4261 tttcaggtgt cgtgaggcta gcatcgattg atcaacaagt ttgtacaaaa aagcaggctg
     4321 gcgccggaac caattcagtc gacaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4381 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4441 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4501 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     4561 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4621 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4681 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4741 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4801 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     4861 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4921 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     4981 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5041 tcggcatgga cgagctgtac aagggccggc ctaccatgtc taagatctct gcagtgagca
     5101 agctcctgct ggccagtcct aaatcccgca gcagcctgtc cacctccacc accaaaaccc
     5161 tcagcttctc caccaagtcc ctgccacagt ctgtgggcca gagctccaac ctaccgccca
     5221 gcgggaaaca catgtcctgg tccacccagt cgctcagcag gaaccggggc tctgggctgg
     5281 cttccaaact gccgctaaga gcggtcaacg ggcgcatttc ggagctgctc cagggcagtg
     5341 cgggcccgag gagtgcacag ctgcgggcag aggcggaaga gcgcagtggg gctccctcgg
     5401 aggacaagcc cgcagccgcc cacctgctgc cctcgcccta cagcaagatc acccctccga
     5461 ggaagccgca ccgctgcagc agcgggcacg gtagtgataa cagcagtgtc ctgagtgggg
     5521 agctaccacc tgccatgggg aagactgccc tcttctacca cagcggaggc agcagtggct
     5581 acgagagcat gatgagagac agcgaggcca ccggcagtgc atcctcagcc caggactcca
     5641 tgagcgagaa cagcagctcc gtgggaggga gatgcaggag cctcaaaaac caaaagaaaa
     5701 ggtccaattc aggttcccag agacggaggc tcattccagc actgtctctc gataccccct
     5761 cacctgtgag gaaaactgcc agcagcacag gggtccgctg ggtggacggc cccttgagga
     5821 gcacccagag gagccttggg gaaccattcg aaattaaagt ctatgagatt gatgacgtgg
     5881 agcgcctgca gcgacgtcga ggagctacaa gcaaggaggt catgtgcttc aatgccaagc
     5941 tgaaaatcct agaacatcgg cagcagagaa tcgctgaagt ccgagccaag tacgagtggc
     6001 tgatgaagga gctagaagcg accaagcagt acctgatgct ggaccccaac aagtggcttc
     6061 gtgaatttga cttggagcag gtcctgcagc tggattccct ggagtacctg gaggcactgg
     6121 agggtgtgac agagcgcttg gagagccgcg tcaacttctg caaggcccat ctcatgatga
     6181 tcacctgctt tgacatcacc tccaggcgcc gataaggcgc gcctctagaa ctatagtgag
     6241 tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca aaccacaact
     6301 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6361 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6421 gttcaggggg aggtgtggga ggttttttaa ttcgcggccg cactcgagat atctagaccc
     6481 agctttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
     6541 cgattctacg tagtaatgaa ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac
     6601 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6661 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6721 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6781 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6841 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6901 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6961 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7021 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7081 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7141 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     7201 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7261 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7321 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7381 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7441 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     7501 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7561 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7621 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7681 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     7741 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7801 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7861 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7921 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7981 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8041 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     8101 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8161 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8221 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8281 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8341 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8401 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8461 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8521 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8581 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8641 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8701 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8761 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8821 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8881 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8941 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9001 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9061 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9121 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9181 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9241 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9301 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9361 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9421 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9481 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9541 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9601 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9661 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9721 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9781 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9841 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9901 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9961 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10021 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10081 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10141 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10201 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10261 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10321 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10381 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10441 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    10501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    10561 acgcaat
//
