LOCUS       sequence-229576-        9250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9250)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/Envelope
FEATURES             Location/Qualifiers
     source          1..9250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3482..3493
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4111..4135)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4188..4229
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4253..4841
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4306..4326)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4724..4735)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4856..5172
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5023..5158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5220..5267
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5286..5684
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5771..6004
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6076..6197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6113..6132)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6167..6186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6237..6372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6392..6411)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6393..6411)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6421..6438)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6421..6437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6430..6452)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6579..7034
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6666..6685)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6876..6897
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7060..7164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7165..7233,7234..8025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7383..7402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8196..8784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8685..8704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8938..8955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9072..9093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9108..9113,9114..9131,9132..9138)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9146..9162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9151..9173
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9170..9186
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9170..9186
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9205..9225
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9207..9225
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctccga attcgccctt aggaggacag
     2581 ctatgatcag gtgcatagga gtcagcaata gggactttgt ggaaggtatg tcaggtggga
     2641 cttgggttga tgttgtcttg gaacatggag gttgtgtcac cgtaatggca caggacaaac
     2701 cgactgtcga catagagctg gttacaacaa cagtcagcaa catggcggag gtaagatcct
     2761 actgctatga ggcatcaata tcagacatgg cttcggacag ccgctgccca acacaaggtg
     2821 aagcctacct tgacaagcaa tcagacactc aatatgtctg caaaagaacg ttagtggaca
     2881 gaggctgggg aaatggatgt ggactttttg gcaaagggag cctggtgaca tgcgctaagt
     2941 ttgcatgctc caagaaaatg accgggaaga gcatccagcc agagaatctg gagtaccgga
     3001 taatgctgtc agttcatggc tcccagcaca gtgggatgat cgttaatgac acaggacatg
     3061 aaactgatga gaatagagcg aaggttgaga taacgcccaa ttcaccaaga gccgaagcca
     3121 ccctgggggg ttttggaagc ctaggacttg attgtgaacc gaggacaggc cttgactttt
     3181 cagatttgta ttacttgact atgaataaca agcactggtt ggtccacaag gagtggttcc
     3241 acgacattcc attaccttgg cacgctgggg cagacaccgg aactccacac tggaacaaca
     3301 aagaagcact ggtagagttc aaggacgcac atgccaaaag gcaaactgtc gtggttctag
     3361 ggagtcaaga aggagcagtt cacacggccc ttgctggagc tctggaggct gagatggatg
     3421 gtgcaaaggg aaggctgtcc tctggccact tgaaatgtcg cctgaaaatg gataaactta
     3481 gattgaaggg cgtgtcatac tccttgtgta ccgcagcgtt cacattcacc aagatcccgg
     3541 ctgaaacact gcacgggaca gtcacagtgg aggtacagta cgcagggaca gatggacctt
     3601 gcaaggttcc agctcagatg gcggtggaca tgcaaactct gaccccagtt gggaggttga
     3661 taaccgctaa ccccgtaatc actgaaagca ctgagaactc taagatgatg ctggaacttg
     3721 atccaccatt tggggactct tacattgtca taggagtcgg ggagaagaag atcacccacc
     3781 actggcacag gagtggcagc accattggaa aagcatttga agccactgtg agaggtgcca
     3841 agagaatggc agtcttggga gacacagcct gggactttgg atcagttgga ggcgctctca
     3901 actcattggg caagggcatc catcaaattt ttggagcagc tttcaaatca ttgtttggag
     3961 gaatgtcctg gttctcacaa atcctcattg gaacgttgct gatgtggttg ggtctgaaca
     4021 caaagaatgg atctatttcc cttatgtgct tggccttagg gggagtgttg atcttcttat
     4081 ccacagccgt ctctgctaag ggcgaattcg acccagcttt cttgtacaaa gtggttgata
     4141 tccagcacag tggcggccgc tcgagtctag aggggccgcg gttcgaaggt aagcctatcc
     4201 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgatcg acaatcaacc
     4261 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4321 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4381 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4441 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4501 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4561 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4621 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4681 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4741 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4801 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggcgatg gtaccggtgt
     4861 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4921 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4981 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5041 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5101 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5161 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     5221 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     5281 aaaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     5341 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     5401 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     5461 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     5521 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     5581 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     5641 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcacaa ttcgagctcg
     5701 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5761 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     5821 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5881 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5941 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6001 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6061 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6121 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6181 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     6241 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6301 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     6361 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     6421 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     6481 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     6541 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     6601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6781 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6901 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     7021 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7081 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     7141 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     7201 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7261 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     7321 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     7381 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     7441 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     7501 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     7561 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7621 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7681 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7741 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7801 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7861 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7921 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7981 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8041 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     8101 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     8161 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     8221 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8281 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     8341 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     8401 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     8461 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     8521 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     8581 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8641 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8701 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8761 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8821 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8881 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8941 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     9001 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     9061 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     9121 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     9181 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     9241 ctgcaagctt
//
