LOCUS       sequence-229578-        9589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9589)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/NS3
FEATURES             Location/Qualifiers
     source          1..9589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4450..4474)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4527..4568
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4592..5180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4645..4665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5063..5074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5195..5511
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5362..5497
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5559..5606
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5625..6023
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6110..6343
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6415..6536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6452..6471)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6506..6525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6576..6711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6731..6750)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6732..6750)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6760..6777)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6760..6776)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6769..6791)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6918..7373
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7005..7024)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7215..7236
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7399..7503
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7504..7572,7573..8364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7722..7741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8535..9123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9024..9043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9277..9294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9411..9432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9447..9452,9453..9470,9471..9477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9485..9501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9490..9512
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9509..9525
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9509..9525
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9544..9564
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9546..9564
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctccga attcgccctt aggaggacag
     2581 ctatgagtgg tgctctatgg gatgtgcctg ctcccaagga agtaaaaaag ggggagacca
     2641 cagatggagt gtacagagta atgactcgta gactgctagg ttcaacacaa gttggagtgg
     2701 gagttatgca agagggggtc tttcacacta tgtggcacgt cacaaaagga tccgcgctga
     2761 gaagcggtga agggagactt gatccatact ggggagatgt caagcaggat ctggtgtcat
     2821 actgtggtcc atggaagcta gatgccgcct gggacgggca cagcgaggtg cagctcttgg
     2881 ccgtgccccc cggagagaga gcgaggaaca tccagactct gcccggaata tttaagacaa
     2941 aggatgggga cattggagcg gttgcgctgg attacccagc aggaacttca ggatctccaa
     3001 tcctagacaa gtgtgggaga gtgataggac tttatggcaa tggggtcgtg atcaaaaatg
     3061 ggagttatgt tagtgccatc acccaaggga ggagggagga agagactcct gttgagtgct
     3121 tcgagccttc gatgctgaag aagaagcagc taactgtctt agacttgcat cctggagctg
     3181 ggaaaaccag gagagttctt cctgaaatag tccgtgaagc cataaaaaca agactccgta
     3241 ctgtgatctt agctccaacc agggttgtcg ctgctgaaat ggaggaagcc cttagagggc
     3301 ttccagtgcg ttatatgaca acagcagtca atgtcaccca ctctggaaca gaaatcgtcg
     3361 acttaatgtg ccatgccacc ttcacttcac gtctactaca gccaatcaga gtccccaact
     3421 ataatctgta tattatggat gaggcccact tcacagatcc ctcaagtata gcagcaagag
     3481 gatacatttc aacaagggtt gagatgggcg aggcggctgc catcttcatg accgccacgc
     3541 caccaggaac ccgtgacgca tttccggact ccaactcacc aattatggac accgaagtgg
     3601 aagtcccaga gagagcctgg agctcaggct ttgattgggt gacggatcat tctggaaaaa
     3661 cagtttggtt tgttccaagc gtgaggaacg gcaatgagat cgcagcttgt ctgacaaagg
     3721 ctggaaaacg ggtcatacag ctcagcagaa agacttttga gacagagttc cagaaaacaa
     3781 aacatcaaga gtgggacttt gtcgtgacaa ctgacatttc agagatgggc gccaacttta
     3841 aagctgaccg tgtcatagat tccaggagat gcctaaagcc ggtcatactt gatggcgaga
     3901 gagtcatttt ggctggaccc atgcctgtca cacatgccag cgctgcccag aggagggggc
     3961 gcataggcag gaatcccaac aaacctggag atgagtatct gtatggaggt gggtgcgcag
     4021 agactgacga agaccatgca cactggcttg aagcaagaat gctccttgac aatatttacc
     4081 tccaagatgg cctcatagcc tcgctctatc gacctgaggc cgacaaagta gcagccattg
     4141 agggagagtt caagcttagg acggagcaaa ggaagacctt tgtggaactc atgaaaagag
     4201 gagatcttcc tgtttggctg gcctatcagg ttgcatctgc cggaataacc tacacagata
     4261 gaagatggtg ctttgatggc acgaccaaca acaccataat ggaagacagt gtgccggcag
     4321 aggtgtggac cagacacgga gagaaaagag tgctcaaacc gaggtggatg gacgccagag
     4381 tttgttcaga tcatgcggcc ctgaagtcat tcaaggagtt tgccgctggg aaaagaaagg
     4441 gcgaattcga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     4501 cgagtctaga ggggccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     4561 attctacgcg taccggttag taatgatcga caatcaacct ctggattaca aaatttgtga
     4621 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4681 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4741 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4801 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4861 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4921 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4981 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5041 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5101 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5161 cctttgggcc gcctccccgc ctggcgatgg taccggtgtg gaaagtcccc aggctcccca
     5221 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5281 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5341 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5401 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     5461 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5521 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     5581 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagcctttgt
     5641 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     5701 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     5761 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     5821 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
     5881 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
     5941 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
     6001 ctggttatgt gtgggagggc taagcacaat tcgagctcgg tacctttaag accaatgact
     6061 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6121 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     6181 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6241 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6301 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     6361 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6421 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6481 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6541 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6601 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6661 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     6721 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     6781 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6841 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6901 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6961 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7021 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7081 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7141 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7201 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7261 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7321 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     7381 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7441 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7501 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7561 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7621 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7681 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7741 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7801 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7861 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7921 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7981 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8041 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8101 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8161 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8221 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8281 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8341 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8401 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8641 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8941 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9121 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9181 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9241 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9301 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9361 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9421 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9481 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9541 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctt
//
