LOCUS       sequence-229580-       10421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10421)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 10421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/NS5
FEATURES             Location/Qualifiers
     source          1..10421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3380..3391)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5282..5306)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5359..5400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5424..6012
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5477..5497)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5895..5906)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6027..6343
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6194..6329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6391..6438
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6457..6855
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6942..7175
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7247..7368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7284..7303)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7338..7357
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7408..7543
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7563..7582)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7564..7582)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7592..7609)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7592..7608)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7601..7623)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7750..8205
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7837..7856)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8047..8068
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8231..8335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8336..8404,8405..9196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8554..8573)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9367..9955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9856..9875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10109..10126
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10243..10264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10279..10284,10285..10302,10303..10309)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10317..10333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10322..10344
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10341..10357
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10341..10357
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10376..10396
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10378..10396
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctagga ggacagctat ggggggtgga
     2581 acaggagaga ccctgggaga gaaatggaag gcccgcttga accagatgtc ggccctggag
     2641 ttctactcct acaaaaagtc aggcatcacc gaggtgtgca gagaagaggc ccgccgcgcc
     2701 ctcaaggatg gtgtggcaac gggaggccat gctgtgtccc gaggaagtgc aaagctgaga
     2761 tggttggtgg agcggggata cctgcagccc tatggaaagg tcattgatct tggatgtggc
     2821 agagggggct ggagttacta cgccgccacc atccgcaaag ttcaagaagt gaaaggatac
     2881 acaaaaggag gccctggtca tgaagaaccc gtgttggtgc aaagctatgg gtggaacata
     2941 gtccgtctta agagtggggt ggacgtcttt catatggcgg ctgagccgtg tgacacgttg
     3001 ctgtgtgaca taggtgagtc atcatctagt cctgaagtgg aagaagcacg gacgctcaga
     3061 gtcctctcca tggtggggga ttggcttgaa aaaagaccag gagccttttg tataaaagtg
     3121 ttgtgcccat acaccagcac tatgatggaa accctggagc gactgcagcg taggtatggg
     3181 ggaggactgg tcagagtgcc actctcccgc aactctacac atgagatgta ctgggtctct
     3241 ggagcgaaaa gcaacaccat aaaaagtgtg tccaccacga gccagctcct cttggggcgc
     3301 atggacgggc ctaggaggcc agtgaaatat gaggaggatg tgaatctcgg ctctggcacg
     3361 cgggctgtgg taagctgcgc tgaagctccc aacatgaaga tcattggtaa ccgcattgaa
     3421 aggatccgca gtgagcacgc ggaaacgtgg ttctttgacg agaaccaccc atataggaca
     3481 tgggcttacc atggaagcta tgaggccccc acacaagggt cagcgtcctc tctaataaac
     3541 ggggttgtca ggctcctgtc aaaaccctgg gatgtggtga ctggagtcac aggaatagcc
     3601 atgaccgaca ccacaccgta tggtcagcaa agagttttca aggaaaaagt ggacactagg
     3661 gtgccagacc cccaagaagg cactcgtcag gttatgagca tggtctcttc ctggttgtgg
     3721 aaagagctag gcaaacacaa acggccacga gtctgtacca aagaagagtt catcaacaag
     3781 gttcgtagca atgcagcatt aggggcaata tttgaagagg aaaaagagtg gaagactgca
     3841 gtggaagctg tgaacgatcc aaggttctgg gctctagtgg acaaggaaag agagcaccac
     3901 ctgagaggag agtgccagag ttgtgtgtac aacatgatgg gaaaaagaga aaagaaacaa
     3961 ggggaatttg gaaaggccaa gggcagccgc gccatctggt atatgtggct aggggctaga
     4021 tttctagagt tcgaagccct tggattcttg aacgaggatc actggatggg gagagagaac
     4081 tcaggaggtg gtgttgaagg gctgggatta caaagactcg gatatgtcct agaagagatg
     4141 agtcgcatac caggaggaag gatgtatgca gatgacactg ctggctggga cacccgcatc
     4201 agcaggttcg atctggagaa tgaagctcta atcaccaacc aaatggagaa agggcatagg
     4261 gccttggcat tggccataat caagtacaca taccaaaaca aagtggtaaa ggtccttaga
     4321 ccagctgaaa aagggaaaac agttatggac attatttcga gacaagacca aagggggagc
     4381 ggacaagttg tcacttacgc tcttaacaca tttaccaacc tagtggtgca actcattcgg
     4441 aatatggagg ctgaggaagt tctagagatg caagacttgt ggctgctgcg gaggtcagag
     4501 aaagtgacca actggttgca gagcaacgga tgggataggc tcaaacgaat ggcagtcagt
     4561 ggagatgatt gcgttgtgaa gccaattgat gataggtttg cacatgccct caggttcttg
     4621 aatgatatgg gaaaagttag gaaggacaca caagagtgga aaccctcaac tggatgggac
     4681 aactgggaag aagttccgtt ttgctcccac cacttcaaca agctccatct caaggacggg
     4741 aggtccattg tggttccctg ccgccaccaa gatgaactga ttggccgggc ccgcgtctct
     4801 ccaggggcgg gatggagcat ccgggagact gcttgcctag caaaatcata tgcgcaaatg
     4861 tggcagctcc tttatttcca cagaagggac ctccgactga tggccaatgc catttgttca
     4921 tctgtgccag ttgactgggt tccaactggg agaactacct ggtcaatcca tggaaaggga
     4981 gaatggatga ccactgaaga catgcttgtg gtgtggaaca gagtgtggat tgaggagaac
     5041 gaccacatgg aagacaagac cccagttacg aaatggacag acattcccta tttgggaaaa
     5101 agggaagact tgtggtgtgg atctctcata gggcacagac cgcgcaccac ctgggctgag
     5161 aacattaaaa acacagtcaa catggtgcgc aggatcatag gtgatgaaga aaagtacatg
     5221 gactacctat ccacccaagt tcgctacttg ggtgaagaag ggtctacacc tggagtgctg
     5281 gacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta
     5341 gaggggccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     5401 cgtaccggtt agtaatgatc gacaatcaac ctctggatta caaaatttgt gaaagattga
     5461 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5521 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5581 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5641 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5701 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5761 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     5821 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5881 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5941 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6001 ccgcctcccc gcctggcgat ggtaccggtg tggaaagtcc ccaggctccc cagcaggcag
     6061 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6121 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6181 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6241 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     6301 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     6361 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     6421 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa
     6481 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
     6541 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     6601 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     6661 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     6721 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     6781 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     6841 gtgtgggagg gctaagcaca attcgagctc ggtaccttta agaccaatga cttacaaggc
     6901 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6961 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7021 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7081 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7141 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7201 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7261 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7321 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7381 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7441 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7501 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     7561 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     7621 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     7681 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     7741 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7801 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7861 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7921 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7981 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8041 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8101 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8161 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     8221 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8281 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8341 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8401 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8461 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8521 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8581 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8641 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8701 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8761 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8821 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8881 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8941 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9001 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9061 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9121 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9181 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9241 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9301 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9421 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9481 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9541 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9601 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9661 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9721 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9781 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9841 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9901 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9961 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    10021 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    10081 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    10141 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    10201 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    10261 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    10321 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
    10381 taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
