LOCUS       sequence-229584-        8941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8941)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/NS4A-2K-NS4B
FEATURES             Location/Qualifiers
     source          1..8941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3802..3826)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3879..3920
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3944..4532
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3997..4017)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4415..4426)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4547..4863
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4714..4849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4911..4958
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4977..5375
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5462..5695
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5767..5888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5804..5823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5858..5877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5928..6063
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6083..6102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6084..6102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6112..6129)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6112..6128)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6121..6143)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6270..6725
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6357..6376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6567..6588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6751..6855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6856..6924,6925..7716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7074..7093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7887..8475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8376..8395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8629..8646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8763..8784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8799..8804,8805..8822,8823..8829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8837..8853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8842..8864
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8861..8877
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8861..8877
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8896..8916
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8898..8916
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctccga attcgccctt aggaggacag
     2581 ctatgggagc ggcttttgga gtgatggaag ccctgggaac actgccagga cacatgacag
     2641 agagattcca ggaagccatt gacaacctcg ctgtgctcat gcgggcagag actggaagca
     2701 ggccttacaa agccgcggcg gcccaattgc cggagaccct agagaccatt atgcttttgg
     2761 ggttgctggg aacagtctcg ctgggaatct ttttcgtctt gatgaggaac aagggcatag
     2821 ggaagatggg ctttggaatg gtgactcttg gggccagcgc atggctcatg tggctctcgg
     2881 aaattgagcc agccagaatt gcatgtgtcc tcattgttgt gttcctattg ctggtggtgc
     2941 tcatacctga gccagaaaag caaagatctc cccaggacaa ccaaatggca atcatcatca
     3001 tggtagcagt aggtcttctg ggcttgatta ccgccaatga actcggatgg ttggagagaa
     3061 caaagagtga cctaagccat ctaatgggaa ggagagagga gggagcaacc ataggattct
     3121 caatggacat tgacctgcgg ccagcctcag cttgggccat ctatgctgcc ttgacaactt
     3181 tcattacccc agccgtccaa catgcagtga ccacttcata caacaactac tccttaatgg
     3241 cgatggccac gcaagctgga gtgttgtttg gtatgggcaa agggatgcca ttctacgcat
     3301 gggactttgg agtcccgctg ctaatgatag gttgctactc acaattaaca cccctgaccc
     3361 taatagtggc catcattttg ctcgtggcgc actacatgta cttgatccca gggctgcagg
     3421 cagcagctgc gcgtgctgcc cagaagagaa cggcagctgg catcatgaag aaccctgttg
     3481 tggatggaat agtggtgact gacattgaca caatgacaat tgacccccaa gtggagaaaa
     3541 agatgggaca ggtgctactc atagcagtag cagtctccag cgccatactg tcgcggaccg
     3601 cctgggggtg gggggaggct ggggccctga tcacagccgc aacttccact ttgtgggaag
     3661 gctctccgaa caagtactgg aactcctcta cagccacttc actgtgtaac atttttaggg
     3721 gaagttactt ggctggagct tctctaatct acatagtaac aagaaacgct ggcttggtca
     3781 agagacgtaa gggcgaattc gacccagctt tcttgtacaa agtggttgat atccagcaca
     3841 gtggcggccg ctcgagtcta gaggggccgc ggttcgaagg taagcctatc cctaaccctc
     3901 tcctcggtct cgattctacg cgtaccggtt agtaatgatc gacaatcaac ctctggatta
     3961 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4021 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4081 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4141 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4201 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4261 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4321 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     4381 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4441 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4501 gagtcggatc tccctttggg ccgcctcccc gcctggcgat ggtaccggtg tggaaagtcc
     4561 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4621 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4681 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4741 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4801 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4861 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     4921 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     4981 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     5041 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     5101 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     5161 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     5221 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     5281 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     5341 aattgctgcc ctctggttat gtgtgggagg gctaagcaca attcgagctc ggtaccttta
     5401 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5461 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5521 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5581 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5641 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5701 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5761 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5821 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5881 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     5941 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6001 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6061 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6121 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6181 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6241 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     6301 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6361 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6421 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6481 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6541 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6601 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6661 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6721 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6781 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6841 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6901 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6961 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7021 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7081 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7141 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7201 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7261 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7321 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7381 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7441 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7501 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7561 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7621 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7681 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7741 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7801 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7861 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7921 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7981 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8041 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8101 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8161 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8221 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8281 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8341 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8401 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8461 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8521 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8581 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8641 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8701 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8761 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8821 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8881 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     8941 t
//
