LOCUS       sequence-229585-       11245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11245)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 11245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/NS5-YFP
FEATURES             Location/Qualifiers
     source          1..11245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3394..3405)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5308..5332)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(5385..5387,5388..5900)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(5427..5448)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5688..5707)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6035..6056
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6106..6130)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6183..6224
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6248..6836
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6301..6321)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6719..6730)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6851..7167
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7018..7153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7215..7262
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7281..7679
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7766..7999
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8071..8192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8108..8127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8162..8181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8232..8367
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8387..8406)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8388..8406)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8416..8433)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8416..8432)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8425..8447)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8574..9029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8661..8680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8871..8892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9055..9159
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9160..9228,9229..10020)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9378..9397)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10191..10779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10680..10699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10933..10950
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11067..11088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11103..11108,11109..11126,11127..11133)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11141..11157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11146..11168
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11165..11181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11165..11181
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11200..11220
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11202..11220
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctccga attcgccctt aggaggacag
     2581 ctatgggggg tggaacagga gagaccctgg gagagaaatg gaaggcccgc ttgaaccaga
     2641 tgtcggccct ggagttctac tcctacaaaa agtcaggcat caccgaggtg tgcagagaag
     2701 aggcccgccg cgccctcaag gatggtgtgg caacgggagg ccatgctgtg tcccgaggaa
     2761 gtgcaaagct gagatggttg gtggagcggg gatacctgca gccctatgga aaggtcattg
     2821 atcttggatg tggcagaggg ggctggagtt actacgccgc caccatccgc aaagttcaag
     2881 aagtgaaagg atacacaaaa ggaggccctg gtcatgaaga acccgtgttg gtgcaaagct
     2941 atgggtggaa catagtccgt cttaagagtg gggtggacgt ctttcatatg gcggctgagc
     3001 cgtgtgacac gttgctgtgt gacataggtg agtcatcatc tagtcctgaa gtggaagaag
     3061 cacggacgct cagagtcctc tccatggtgg gggattggct tgaaaaaaga ccaggagcct
     3121 tttgtataaa agtgttgtgc ccatacacca gcactatgat ggaaaccctg gagcgactgc
     3181 agcgtaggta tgggggagga ctggtcagag tgccactctc ccgcaactct acacatgaga
     3241 tgtactgggt ctctggagcg aaaagcaaca ccataaaaag tgtgtccacc acgagccagc
     3301 tcctcttggg gcgcatggac gggcctagga ggccagtgaa atatgaggag gatgtgaatc
     3361 tcggctctgg cacgcgggct gtggtaagct gcgctgaagc tcccaacatg aagatcattg
     3421 gtaaccgcat tgaaaggatc cgcagtgagc acgcggaaac gtggttcttt gacgagaacc
     3481 acccatatag gacatgggct taccatggaa gctatgaggc ccccacacaa gggtcagcgt
     3541 cctctctaat aaacggggtt gtcaggctcc tgtcaaaacc ctgggatgtg gtgactggag
     3601 tcacaggaat agccatgacc gacaccacac cgtatggtca gcaaagagtt ttcaaggaaa
     3661 aagtggacac tagggtgcca gacccccaag aaggcactcg tcaggttatg agcatggtct
     3721 cttcctggtt gtggaaagag ctaggcaaac acaaacggcc acgagtctgt accaaagaag
     3781 agttcatcaa caaggttcgt agcaatgcag cattaggggc aatatttgaa gaggaaaaag
     3841 agtggaagac tgcagtggaa gctgtgaacg atccaaggtt ctgggctcta gtggacaagg
     3901 aaagagagca ccacctgaga ggagagtgcc agagttgtgt gtacaacatg atgggaaaaa
     3961 gagaaaagaa acaaggggaa tttggaaagg ccaagggcag ccgcgccatc tggtatatgt
     4021 ggctaggggc tagatttcta gagttcgaag cccttggatt cttgaacgag gatcactgga
     4081 tggggagaga gaactcagga ggtggtgttg aagggctggg attacaaaga ctcggatatg
     4141 tcctagaaga gatgagtcgc ataccaggag gaaggatgta tgcagatgac actgctggct
     4201 gggacacccg catcagcagg ttcgatctgg agaatgaagc tctaatcacc aaccaaatgg
     4261 agaaagggca tagggccttg gcattggcca taatcaagta cacataccaa aacaaagtgg
     4321 taaaggtcct tagaccagct gaaaaaggga aaacagttat ggacattatt tcgagacaag
     4381 accaaagggg gagcggacaa gttgtcactt acgctcttaa cacatttacc aacctagtgg
     4441 tgcaactcat tcggaatatg gaggctgagg aagttctaga gatgcaagac ttgtggctgc
     4501 tgcggaggtc agagaaagtg accaactggt tgcagagcaa cggatgggat aggctcaaac
     4561 gaatggcagt cagtggagat gattgcgttg tgaagccaat tgatgatagg tttgcacatg
     4621 ccctcaggtt cttgaatgat atgggaaaag ttaggaagga cacacaagag tggaaaccct
     4681 caactggatg ggacaactgg gaagaagttc cgttttgctc ccaccacttc aacaagctcc
     4741 atctcaagga cgggaggtcc attgtggttc cctgccgcca ccaagatgaa ctgattggcc
     4801 gggcccgcgt ctctccaggg gcgggatgga gcatccggga gactgcttgc ctagcaaaat
     4861 catatgcgca aatgtggcag ctcctttatt tccacagaag ggacctccga ctgatggcca
     4921 atgccatttg ttcatctgtg ccagttgact gggttccaac tgggagaact acctggtcaa
     4981 tccatggaaa gggagaatgg atgaccactg aagacatgct tgtggtgtgg aacagagtgt
     5041 ggattgagga gaacgaccac atggaagaca agaccccagt tacgaaatgg acagacattc
     5101 cctatttggg aaaaagggaa gacttgtggt gtggatctct catagggcac agaccgcgca
     5161 ccacctgggc tgagaacatt aaaaacacag tcaacatggt gcgcaggatc ataggtgatg
     5221 aagaaaagta catggactac ctatccaccc aagttcgcta cttgggtgaa gaagggtcta
     5281 cacctggagt gctgaagggc gaattcgacc cagctttctt gtacaaagtg gttgatatcc
     5341 agcacagtgg cggccgctcg agtctagagg ggccgcggtt cgaagtgagc aagggcgagg
     5401 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     5461 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     5521 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     5581 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     5641 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     5701 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5761 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     5821 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     5881 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     5941 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     6001 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     6061 ccgccgggat cactctcggc atggacgagc tgaagggcga attcgaccca gctttcttgt
     6121 acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggg ccgcggttcg
     6181 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat
     6241 gatcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6301 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6361 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6421 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6481 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6541 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6601 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     6661 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6721 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6781 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     6841 cgatggtacc ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6901 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6961 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7021 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7081 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7141 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     7201 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     7261 acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg
     7321 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     7381 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     7441 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     7501 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     7561 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     7621 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     7681 cacaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7741 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7801 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7861 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7921 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7981 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8041 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     8101 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8161 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8221 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8281 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8341 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     8401 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8461 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8521 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     8581 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8641 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8701 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8761 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8821 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8881 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8941 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     9001 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     9061 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9121 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9181 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9241 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9301 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9361 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9421 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9481 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9541 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9601 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9661 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9721 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9781 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9841 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9901 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9961 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10021 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10081 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10141 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10201 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10261 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10321 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10381 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10441 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10501 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10561 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10621 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10681 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10741 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10801 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    10861 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    10921 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    10981 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
    11041 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    11101 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    11161 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
    11221 gaacaaaagc tggagctgca agctt
//
