LOCUS       sequence-229603-        9045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ectopic expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, 
            Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J
  TITLE     Infection with a Brazilian isolate of Zika virus generates RIG-I 
            stimulatory RNA and the viral NS5 protein blocks type I IFN 
            induction and signaling.
  JOURNAL   Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483.
  PUBMED    29572905
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483."
COMMENT     Sequence Label: pcDNA3.2/NS5-YFP-NLS-mut
FEATURES             Location/Qualifiers
     source          1..9045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    911..935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1773..1784)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3687..3711)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3764..3766,3767..4279)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(3806..3827)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4067..4086)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4414..4435
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4485..4509)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4535..4576
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4616..4635)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4660..4708
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4910..5338
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4997..5016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5207..5228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5347..5367)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5352..5681
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5532..5667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5594..5613
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5748..6542
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5802..5821)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6412..6431
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6718..6839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6755..6774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6809..6828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6888..6904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6888..6904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6901..6923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6912..6928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6936..6942,6943..6960,6961..6966))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6981..7002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7119..7136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7290..7878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7370..7389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8049..8840,8841..8909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8672..8691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8910..9014)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagctatca acaagtttgt acaaaaaagc aggctccgaa ttcgccctta ggaggacagc
      961 tatggggggt ggaacaggag agaccctggg agagaaatgg aaggcccgct tgaaccagat
     1021 gtcggccctg gagttctact cctacaaaaa gtcaggcatc accgaggtgt gcagagaaga
     1081 ggcccgccgc gccctcaagg atggtgtggc aacgggaggc catgctgtgt cccgaggaag
     1141 tgcaaagctg agatggttgg tggagcgggg atacctgcag ccctatggaa aggtcattga
     1201 tcttggatgt ggcagagggg gctggagtta ctacgccgcc accatccgca aagttcaaga
     1261 agtgaaagga tacacaaaag gaggccctgg tcatgaagaa cccgtgttgg tgcaaagcta
     1321 tgggtggaac atagtccgtc ttaagagtgg ggtggacgtc tttcatatgg cggctgagcc
     1381 gtgtgacacg ttgctgtgtg acataggtga gtcatcatct agtcctgaag tggaagaagc
     1441 acggacgctc agagtcctct ccatggtggg ggattggctt gaaaaaagac caggagcctt
     1501 ttgtataaaa gtgttgtgcc catacaccag cactatgatg gaaaccctgg agcgactgca
     1561 gcgtaggtat gggggaggac tggtcagagt gccactctcc cgcaactcta cacatgagat
     1621 gtactgggtc tctggagcga aaagcaacac cataaaaagt gtgtccacca cgagccagct
     1681 cctcttgggg cgcatggacg ggcctaggag gccagtgaaa tatgaggagg atgtgaatct
     1741 cggctctggc acgcgggctg tggtaagctg cgctgaagct cccaacatga agatcattgg
     1801 taaccgcatt gaaaggatcc gcagtgagca cgcggaaacg tggttctttg acgagaacca
     1861 cccatatagg acatgggctt accatggaag ctatgaggcc cccacacaag ggtcagcgtc
     1921 ctctctaata aacggggttg tcaggctcct gtcaaaaccc tgggatgtgg tgactggagt
     1981 cacaggaata gccatgaccg acaccacacc gtatggtcag caaagagttt tcaaggaaaa
     2041 agtggacact agggtgccag acccccaaga aggcactgct gcggttatga gcatggtctc
     2101 ttcctggttg tggaaagagc taggcgcagc cgcacggcca cgagtctgta ccaaagaaga
     2161 gttcatcaac aaggttcgta gcaatgcagc attaggggca atatttgaag aggaaaaaga
     2221 gtggaagact gcagtggaag ctgtgaacga tccaaggttc tgggctctag tggacaagga
     2281 aagagagcac cacctgagag gagagtgcca gagttgtgtg tacaacatga tgggaaaaag
     2341 agaaaagaaa caaggggaat ttggaaaggc caagggcagc cgcgccatct ggtatatgtg
     2401 gctaggggct agatttctag agttcgaagc ccttggattc ttgaacgagg atcactggat
     2461 ggggagagag aactcaggag gtggtgttga agggctggga ttacaaagac tcggatatgt
     2521 cctagaagag atgagtcgca taccaggagg aaggatgtat gcagatgaca ctgctggctg
     2581 ggacacccgc atcagcaggt tcgatctgga gaatgaagct ctaatcacca accaaatgga
     2641 gaaagggcat agggccttgg cattggccat aatcaagtac acataccaaa acaaagtggt
     2701 aaaggtcctt agaccagctg aaaaagggaa aacagttatg gacattattt cgagacaaga
     2761 ccaaaggggg agcggacaag ttgtcactta cgctcttaac acatttacca acctagtggt
     2821 gcaactcatt cggaatatgg aggctgagga agttctagag atgcaagact tgtggctgct
     2881 gcggaggtca gagaaagtga ccaactggtt gcagagcaac ggatgggata ggctcaaacg
     2941 aatggcagtc agtggagatg attgcgttgt gaagccaatt gatgataggt ttgcacatgc
     3001 cctcaggttc ttgaatgata tgggaaaagt taggaaggac acacaagagt ggaaaccctc
     3061 aactggatgg gacaactggg aagaagttcc gttttgctcc caccacttca acaagctcca
     3121 tctcaaggac gggaggtcca ttgtggttcc ctgccgccac caagatgaac tgattggccg
     3181 ggcccgcgtc tctccagggg cgggatggag catccgggag actgcttgcc tagcaaaatc
     3241 atatgcgcaa atgtggcagc tcctttattt ccacagaagg gacctccgac tgatggccaa
     3301 tgccatttgt tcatctgtgc cagttgactg ggttccaact gggagaacta cctggtcaat
     3361 ccatggaaag ggagaatgga tgaccactga agacatgctt gtggtgtgga acagagtgtg
     3421 gattgaggag aacgaccaca tggaagacaa gaccccagtt acgaaatgga cagacattcc
     3481 ctatttggga aaaagggaag acttgtggtg tggatctctc atagggcaca gaccgcgcac
     3541 cacctgggct gagaacatta aaaacacagt caacatggtg cgcaggatca taggtgatga
     3601 agaaaagtac atggactacc tatccaccca agttcgctac ttgggtgaag aagggtctac
     3661 acctggagtg ctgaagggcg aattcgaccc agctttcttg tacaaagtgg ttgatatcca
     3721 gcacagtggc ggccgctcga gtctagaggg gccgcggttc gaagtgagca agggcgagga
     3781 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3841 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3901 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     3961 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4021 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4081 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4141 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4201 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4261 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4321 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     4381 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4441 cgccgggatc actctcggca tggacgagct gaagggcgaa ttcgacccag ctttcttgta
     4501 caaagtggtt gatctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     4561 cggtctcgat tctacgcgta ccggttagta atgagtttaa acgggggagg ctaactgaaa
     4621 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     4681 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     4741 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     4801 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     4861 ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt
     4921 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4981 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5041 tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg
     5101 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5161 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5221 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5281 gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     5341 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5401 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5521 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5581 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     5641 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     5701 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5761 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5821 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5881 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     5941 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6001 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6061 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6121 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6181 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6241 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6301 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6361 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6421 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6481 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6541 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     6601 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     6661 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     6721 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6781 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6841 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6901 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6961 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7021 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7081 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7141 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7201 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7261 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7321 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7381 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7441 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     7501 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7561 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7621 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7681 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     7741 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7801 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7861 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7921 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7981 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8041 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8101 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8161 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8221 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8281 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8341 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8401 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8461 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8521 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8581 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8641 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8701 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8761 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8821 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8881 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8941 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9001 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
