LOCUS       sequence-229606-        4899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible donor vector with KAT14-1-546 truncation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Viita T, Kyheroinen S, Prajapati B, Virtanen J, Frilander MJ, 
            Varjosalo M, Vartiainen MK
  TITLE     Nuclear actin interactome analysis links actin to KAT14 histone 
            acetyl transferase and mRNA splicing.
  JOURNAL   J Cell Sci. 2019 Apr 17;132(8). pii: jcs.226852. doi: 
            10.1242/jcs.226852.
  PUBMED    30890647
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-04-17"; volume: "132(8). pii: jcs.226852. doi"; 
            pages: " 10.1242/jcs.226852"
COMMENT     Sequence Label: pENTR-KAT14-1-546
FEATURES             Location/Qualifiers
     source          1..4899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3024..3123)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3140..3159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3141..3159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3164..3180)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3164..3180)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3282..3301)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3293..4102
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3360..3379)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4249..4837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4738..4757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggata gtagcatcca cctgagtagt ctgatcagtc ggcatgatga
      721 cgaagccacg agaacatcga cctcagaagg actggaggaa ggtgaagtgg agggagagac
      781 gctcctgatc gtcgaatccg aggatcaggc atcagtggac ttatcgcacg accagagtgg
      841 ggattccctc aacagtgatg aaggagacgt gtcttggatg gaggagcagc tgtcctactt
      901 ctgtgacaag tgccaaaaat ggataccagc cagtcagctg agggaacagc tcagttacct
      961 taagggtgat aattttttta ggtttacttg ttcggattgc tcagcagatg gcaaggagca
     1021 gtatgaaagg ctgaagctga catggcagca agtcgtcatg ttggcaatgt acaacttgtc
     1081 tctggaagga agtggacgtc aaggttattt caggtggaaa gaagatatct gtgcttttat
     1141 tgagaaacat tggacttttt tactagggaa taggaaaaag acgtctacct ggtggagcac
     1201 cgtggcaggt tgcctcagcg tgggaagtcc catgtacttc cgttcaggtg ctcaggaatt
     1261 tggagagcca ggatggtgga aacttgttca taacaagccc ccaacgatga aacctgaagg
     1321 agagaagttg tctgcctcta ctttgaaaat aaaagcagcc tcaaaaccaa ctttagatcc
     1381 catcattact gttgagggac ttagaaaacg agcaagtcgg aatcctgtgg aatctgccat
     1441 ggaattaaaa gagaaaaggt ctcgaactca ggaagcaaaa gacattagaa gagcccagaa
     1501 ggaggccgct ggctttcttg acaggagcac atcttctacc cctgtaaaat tcataagccg
     1561 aggccgcagg ccagatgtga ttctggaaaa aggcgaagtg attgactttt cctccttgag
     1621 ctcctctgac cgcaccccgc tgacaagccc atctccttct ccttctctgg atttctctgc
     1681 ccctggtaca cctgcctctc attctgccac acctagcttg ctttcagaag cagatctgat
     1741 tccagatgtg atgcccccac aagccttgtt tcatgatgac gatgagatgg aaggcgatgg
     1801 agtcatagac ccagggatgg agtacgtccc accccctgct gggtcagtag cttctgggcc
     1861 agtggttggg ggcagaaaga aggtcagagg ccctgaacag ataaagcagg aggtagagag
     1921 tgaggaggaa aaacccgaca ggatggatat tgacagtgaa gacacagatt caaacacatc
     1981 tttgcaaaca agggctagag aaaagaggaa gcctcagctg gagaaggaca caaagccgaa
     2041 agagcccagg tatactcccg tgagcatcta cgaggaaaag ctgctgctca agaggctgga
     2101 agcttgtccc ggtgctgttg ccatgactcc ggaagctcgg agactgaaac gcaaactgat
     2161 tgtcagacaa gcgaaaaggg ataggggatt accacttttt gacttggatc aagttgttaa
     2221 tgctgctctt ttgttagttg acgggattta tggagccaaa gaaggaggaa tttccagact
     2281 tccagctgga caagccacgt acagaaccac ctgataggac ttcagaatcc ttgaccgata
     2341 ccagacttcc ttgccgtcca ggaagggatt tcgacaccag accaccaagt ttttgtatcg
     2401 cttggtagga tcagaagata tggctgtgga ccagagtatt gtcagccctt atacctctcg
     2461 gatcttgaaa ccttatatca ggcgtgatta tgaaacaaag ccacccaaac tgcagctcct
     2521 gtcacagatt cgttcccacc tgcacaggag cgaccctcac tggacgccgg agcccgacgc
     2581 acctctcgat tactgttatg tgcggccaaa tcacatccca acgatcaact ccatgtgtca
     2641 ggagtttttt tggcctggca ttgacctgtc tgagtgtctg cagtacccag acttcagtgt
     2701 tgttgttctt tataaaaaag tcatcattgc ctttggcttc atggttcctg atgtgaaata
     2761 caatgaagct tacatttcat ttctgttcgt ccaccctgaa tggagaagag cagggattgc
     2821 aactttcatg atctatcatc tgattcagac ctgcatgggc aaggacgtaa cccttcacgt
     2881 ctcagcaagc aaccccgcta tgctactgta ccagaagttt ggattcaaga ctgaagaata
     2941 tgtattagat ttctatgata aatattaccc attggagagt acagagtgta aacacgcatt
     3001 ctttctgagg ctccggcgct aggacccagc tttcttgtac aaagttggca ttataagaaa
     3061 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     3121 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     3181 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     3241 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     3301 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     3361 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     3421 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     3481 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     3541 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     3601 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     3661 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     3721 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     3781 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     3841 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     3901 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     3961 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     4021 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     4081 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     4141 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
     4201 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4261 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4321 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4381 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4441 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4501 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4561 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4621 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4681 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4741 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4801 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4861 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
