LOCUS       sequence-229611-        6097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter gene expression in S. rosetta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6097)
  TITLE     King Lab plasmids
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NK690
FEATURES             Location/Qualifiers
     source          1..6097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5703..5720)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5703..5719)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5712..5734)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5928..5947)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6047..6069
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatc ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gtaacgactg cagtcttgtt gtcgtttgtc gtcgtcgttc
     2281 tctgtctccc acccaacttt tgccaattgc aacagtgact tctgtacaat ttgcaacaga
     2341 taccaacgtg tcgagtcagc acaaatggcg aggaaggttc gcgtgcattg cttgcgcgcc
     2401 tctttgtcat gggcgcacgc tgatttgtgt gtgatttgca ccaaatttcc tgccgacaca
     2461 acttgggccc gtaccacacg acccgtttct ggacccgttt ctggcccaga aacgcgaatg
     2521 ttgcgaaaga tgcgaccacg tgctcgctgt cgacaacaca aactcccgca caatccccat
     2581 gaaaaaaaaa gtgcttgctg ggcttgtgtc tggcctgtgt gcgtggcgtg gccgcgtgtg
     2641 gcttcgttct tgcttctgct cactcagcct gttacacgca gagtggctgc gagaaacaag
     2701 gagaggggtt tgcggcattg gcgcatgtgc atctgccttt ttcattgagt cagtctgcag
     2761 ctcaagaggt tggtttcgtc gacggatatg agtgagcttc tatggatgat gagaggtggt
     2821 ggtggttgta tggttggtgg ttggtggttg gtgcaaggcg agccacgcga agagcgacaa
     2881 ggatgaggga gaggagagga gactaggaaa gaggtgaagg gaagggaagg gcgagcaatg
     2941 acgacgagat aaaagacagc caccaccgca cccgcacaaa agcattgctc gcacgcatgg
     3001 tcgtgtgtta tgtttcacac caccaacata accaatacaa gccatcacca atacaagcca
     3061 gcgctcaagc acacacgcac gcacgcacac acaatacaca cgcacgtgaa acgttcacat
     3121 tgtccgctca caaatgcctt tcccttctcg tcgctttcct tgcctttttt tcttcccctc
     3181 ctgacttcct tcctgccttc cttccttcct ccccttctcc gtccaccaac accatttatt
     3241 tctctgctct ttttcctcgt gccacgtgag cagtcttttc gcgtcgtctt cttctttgac
     3301 cccgtttacc gtcaacacca tcaacaacca gcaacatgtt gagggtacga ggaagatcgc
     3361 tggaggcccg gtttgtgtac atcatcgtgc tggctgtggt gtacacggcc acgtacatgt
     3421 acagcgccac atccggtgac ccaagcgtac cggttggcgg cgcagacatg ccgccgtggc
     3481 tcgcacagct gggacgacca cgcgcagttg cagaggagct ggcagaacag caacagctgc
     3541 ttgacgcgac gcggcgcagc gacgacggag ccaacagaca cagcaacgat ggcgccgccg
     3601 cagtctcaca acaccagcag caccagcagc aggcggagca cgatctggcc agcacacgga
     3661 gtccaaagac accgacgcgg caagaggaca aaggcgtgga cgagaaggag aaaggcatga
     3721 agttcgaggt ggaggggggc gaagatggat tcgtgcccag ggagcacatg aagaatgcga
     3781 tgggtatgcc agacactgca gaggccgacc cgtggaagcg gcgcaggcag gacaagtggc
     3841 gcctcatcat cttgttccag cacaacttcc ccatcctgaa gcaggcggta gaagggttcc
     3901 agcgtgccag cgacatcatg gtgcccagca tgatcatcgt cgacaactcc aaggacaggg
     3961 atgcctcgaa cagcgtgtgg cttgtggagc gggtggcaga ggtggtcgtg ccagaccgcc
     4021 agctaaactt cccagagctg cacaactaca tggccacgct ggcactcgag cgccggctcg
     4081 aattctactt ctgggcccac gcagacaact acgtgctgcc gctggagcct ggccgcgaca
     4141 tgggcaagga cgccatcgac tgcctgcgcg agcagatggc gcggttcccc aactggggca
     4201 tgatcctctt cgcctacgac cacctggccg cctaccgcac gcagacgcta gtccaggtgc
     4261 catgggaccc acacgtgttc cagtacggct cggagtgcga tgcgtatggg cgcatccgcg
     4321 atgcgggcta cgacgcgcgc gcgtgcaaga ttcactactc gtacgacatg aagcgggtga
     4381 ttggcatcct ggacacggac tcgtacgacc gggtcaaggc gaagctggac gaggaggcaa
     4441 aggacaagtc caagcgcaac cagtggcgcg agggagccat gtccgagaag gagcagcaat
     4501 ggcgcaagcg catgaaggag gcgtcacgcg catacttggc cgagaagtgg ggcgaggtga
     4561 agtgcaagct gcgcggtgtg ccgtgccaca agccctggcc ttactgccca acgtgcccca
     4621 cagaccttcc ccagtgctac ggcaaggagg cgacgtggga gcaactcgac cacattcacg
     4681 ccagggtgca ccaggtgttt gacaatgatc ccgacaagcc accaccgctc gaggcaggcg
     4741 gtagcggtgg ctccatggtc tccaagggcg aggagaccac gatgggcgtc atcaagcccg
     4801 acatgaagat caagctcaag atggagggca acgtcaacgg ccacgccttc gtcatcgagg
     4861 gcgagggcga gggcaagccc tacgacggca ccaacaccat caaccttgag gtcaaagaag
     4921 gtgctcctct gccctttagc tacgacatcc tcaccaccgc cttctcctac ggcaaccgcg
     4981 ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc cccgagggct
     5041 actcctggga gcgcaccatg accttcgagg acaagggcat cgtcaaggtc aagtccgaca
     5101 tctctatgga ggaggactcc ttcatctacg agatccacct caagggcgag aacttccctc
     5161 ccaacggccc cgtcatgcag aaggagacta cgggttggga cgcctccacc gagcgcatgt
     5221 acgtccgcga cggtgttctc aagggcgacg tgaagatgaa acttctcctt gagggcggcg
     5281 gccaccaccg cgtcgacttc aagaccatct accgcgccaa gaaggccgtc aagctccccg
     5341 actaccactt cgtcgaccac cgcatcgaga tcctcaacca cgacaaggac tacaacaaag
     5401 tcactgtcta cgagatcgcc gtcgcccgca actctaccga cggcatggac gagctttaca
     5461 aggcttaagc gcctcgttgg tcattgccac cttgttgcct cttttgctgt tgtcctcgtg
     5521 tcgacgcgtg ccacgtgcgc ttgttgtgag ttgattctgc ttcttgtgca cgtcatgccg
     5581 agcccttggg ccccgcaata aacccactat gccagtgtaa ctatccttcc ccccaatgta
     5641 taactgtgac gatcaatgag cggtgcccgt ctagaggatc cccgggtacc gagctcgaat
     5701 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5761 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5821 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     5881 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     5941 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6001 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     6061 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
