LOCUS       sequence-229622-        3738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible donor vector with nuclear localization signal 
            (NLS) attached to WT actin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Viita T, Kyheroinen S, Prajapati B, Virtanen J, Frilander MJ, 
            Varjosalo M, Vartiainen MK
  TITLE     Nuclear actin interactome analysis links actin to KAT14 histone 
            acetyl transferase and mRNA splicing.
  JOURNAL   J Cell Sci. 2019 Apr 17;132(8). pii: jcs.226852. doi: 
            10.1242/jcs.226852.
  PUBMED    30890647
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-04-17"; volume: "132(8). pii: jcs.226852. doi"; 
            pages: " 10.1242/jcs.226852"
COMMENT     Sequence Label: pENTR-NLS-actin
FEATURES             Location/Qualifiers
     source          1..3738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             692..712
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1863..1962)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1979..1998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1980..1998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2003..2019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2003..2019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2121..2140)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2132..2941
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2199..2218)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3088..3676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3577..3596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat gcctaagaaa aagcggaagg tgggatccat
      721 ggatgatgat atcgccgcgc tcgtcgtcga caacggctcc ggcatgtgca aggccggctt
      781 cgcgggcgac gatgcccccc gggccgtctt cccctccatc gtggggcgcc ccaggcacca
      841 gggcgtgatg gtgggcatgg gtcagaagga ttcctatgtg ggcgacgagg cccagagcaa
      901 gagaggcatc ctcaccctga agtaccccat cgagcacggc atcgtcacca actgggacga
      961 catggagaaa atctggcacc acaccttcta caatgagctg cgtgtggctc ccgaggagca
     1021 ccccgtgctg ctgaccgagg cccccctgaa ccccaaggcc aaccgcgaga agatgaccca
     1081 gatcatgttt gagaccttca acaccccagc catgtacgtt gctatccagg ctgtgctatc
     1141 cctgtacgcc tctggccgta ccactggcat cgtgatggac tccggtgacg gggtcaccca
     1201 cactgtgccc atctacgagg ggtatgccct cccccatgcc atcctgcgtc tggacctggc
     1261 tggccgggac ctgactgact acctcatgaa gatcctcacc gagcgcggct acagcttcac
     1321 caccacggcc gagcgggaaa tcgtgcgtga cattaaggag aagctgtgct acgtcgccct
     1381 ggacttcgag caagagatgg ccacggctgc ttccagctcc tccctggaga agagctacga
     1441 gctgcctgac ggccaggtca tcaccattgg caatgagcgg ttccgctgcc ctgaggcact
     1501 cttccagcct tccttcctgg gcatggagtc ctgtggcatc cacgaaacta ccttcaactc
     1561 catcatgaag tgtgacgtgg acatccgcaa agacctgtac gccaacacag tgctgtctgg
     1621 cggcaccacc atgtaccctg gcattgccga caggatgcag aaggagatca ctgccctggc
     1681 acccagcaca atgaagatca agatcattgc tcctcctgag cgcaagtact ccgtgtggat
     1741 cggcggctcc atcctggcct cgctgtccac cttccagcag atgtggatca gcaagcagga
     1801 gtatgacgag tccggcccct ccatcgtcca ccgcaaatgc ttctagaagg gtgggcgcgc
     1861 cgacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1921 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1981 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2041 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     2101 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2161 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2221 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     2281 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2341 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2401 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2461 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2521 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2581 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2641 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2701 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     2761 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     2821 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     2881 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     2941 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3001 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3061 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3241 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3721 gccttttgct cacatgtt
//
