LOCUS       sequence-229630-        7248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIS-tagged insect cell expression vector with KAT14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Viita T, Kyheroinen S, Prajapati B, Virtanen J, Frilander MJ, 
            Varjosalo M, Vartiainen MK
  TITLE     Nuclear actin interactome analysis links actin to KAT14 histone 
            acetyl transferase and mRNA splicing.
  JOURNAL   J Cell Sci. 2019 Apr 17;132(8). pii: jcs.226852. doi: 
            10.1242/jcs.226852.
  PUBMED    30890647
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-04-17"; volume: "132(8). pii: jcs.226852. doi"; 
            pages: " 10.1242/jcs.226852"
COMMENT     Sequence Label: KAT14-pDEST10
FEATURES             Location/Qualifiers
     source          1..7248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..207
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     132..149
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             274..291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             313..333
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    337..361
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2717..2741)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    2969..3103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3006..3025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3060..3079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3132..3297
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3481..3935
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3568..3587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3777..3798
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3961..4065
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4066..4134,4135..4926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4284..4303)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5097..5685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5586..5605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5839..5856
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5990..6214
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6281..6814)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6984..6989,6990..7006,7007..7012))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc
       61 aggactctag ctatagttct agtggttggc tacgtatact ccggaatatt aatagatcat
      121 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      181 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      241 cgcggatctc ggtccgaaac catgtcgtac taccatcacc atcaccatca cgattacgat
      301 atcccaacga ccgaaaacct gtattttcag ggcatcacaa gtttgtacaa aaaagcaggc
      361 tccaccatgg atagtagcat ccacctgagt agtctgatca gtcggcatga tgacgaagcc
      421 acgagaacat cgacctcaga aggactggag gaaggtgaag tggagggaga gacgctcctg
      481 atcgtcgaat ccgaggatca ggcatcagtg gacttatcgc acgaccagag tggggattcc
      541 ctcaacagtg atgaaggaga cgtgtcttgg atggaggagc agctgtccta cttctgtgac
      601 aagtgccaaa aatggatacc agccagtcag ctgagggaac agctcagtta ccttaagggt
      661 gataattttt ttaggtttac ttgttcggat tgctcagcag atggcaagga gcagtatgaa
      721 aggctgaagc tgacatggca gcaagtcgtc atgttggcaa tgtacaactt gtctctggaa
      781 ggaagtggac gtcaaggtta tttcaggtgg aaagaagata tctgtgcttt tattgagaaa
      841 cattggactt ttttactagg gaataggaaa aagacgtcta cctggtggag caccgtggca
      901 ggttgcctca gcgtgggaag tcccatgtac ttccgttcag gtgctcagga atttggagag
      961 ccaggatggt ggaaacttgt tcataacaag cccccaacga tgaaacctga aggagagaag
     1021 ttgtctgcct ctactttgaa aataaaagca gcctcaaaac caactttaga tcccatcatt
     1081 actgttgagg gacttagaaa acgagcaagt cggaatcctg tggaatctgc catggaatta
     1141 aaagagaaaa ggtctcgaac tcaggaagca aaagacatta gaagagccca gaaggaggcc
     1201 gctggctttc ttgacaggag cacatcttct acccctgtaa aattcataag ccgaggccgc
     1261 aggccagatg tgattctgga aaaaggcgaa gtgattgact tttcctcctt gagctcctct
     1321 gaccgcaccc cgctgacaag cccatctcct tctccttctc tggatttctc tgcccctggt
     1381 acacctgcct ctcattctgc cacacctagc ttgctttcag aagcagatct gattccagat
     1441 gtgatgcccc cacaagcctt gtttcatgat gacgatgaga tggaaggcga tggagtcata
     1501 gacccaggga tggagtacgt cccaccccct gctgggtcag tagcttctgg gccagtggtt
     1561 gggggcagaa agaaggtcag aggccctgaa cagataaagc aggaggtaga gagtgaggag
     1621 gaaaaacccg acaggatgga tattgacagt gaagacacag attcaaacac atctttgcaa
     1681 acaagggcta gagaaaagag gaagcctcag ctggagaagg acacaaagcc gaaagagccc
     1741 aggtatactc ccgtgagcat ctacgaggaa aagctgctgc tcaagaggct ggaagcttgt
     1801 cccggtgctg ttgccatgac tccggaagct cggagactga aacgcaaact gattgtcaga
     1861 caagcgaaaa gggatagggg attaccactt tttgacttgg atcaagttgt taatgctgct
     1921 cttttgttag ttgacgggat ttatggagcc aaagaaggag gaatttccag acttccagct
     1981 ggacaagcca cgtacagaac cacctgtcag gacttcagaa tccttgaccg ataccagact
     2041 tccttgccgt ccaggaaggg atttcgacac cagaccacca agtttttgta tcgcttggta
     2101 ggatcagaag atatggctgt ggaccagagt attgtcagcc cttatacctc tcggatcttg
     2161 aaaccttata tcaggcgtga ttatgaaaca aagccaccca aactgcagct cctgtcacag
     2221 attcgttccc acctgcacag gagcgaccct cactggacgc cggagcccga cgcacctctc
     2281 gattactgtt atgtgcggcc aaatcacatc ccaacgatca actccatgtg tcaggagttt
     2341 ttttggcctg gcattgacct gtctgagtgt ctgcagtacc cagacttcag tgttgttgtt
     2401 ctttataaaa aagtcatcat tgcctttggc ttcatggttc ctgatgtgaa atacaatgaa
     2461 gcttacattt catttctgtt cgtccaccct gaatggagaa gagcagggat tgcaactttc
     2521 atgatctatc atctgattca gacctgcatg ggcaaggacg taacccttca cgtctcagca
     2581 agcaaccccg ctatgctact gtaccagaag tttggattca agactgaaga atatgtatta
     2641 gatttctatg ataaatatta cccattggag agtacagagt gtaaacacgc attctttctg
     2701 aggctccggc gctaggaccc agctttcttg tacaaagtgg tgatgccatg gatccggaat
     2761 tcaaaggcct acgtcgacga gctcaactag tgcggccgct ttcgaatcta gagcctgcag
     2821 tctcgaggca tgcggtacca agcttgtcga gaagtactag aggatcataa tcagccatac
     2881 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2941 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3001 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3061 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     3121 taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt
     3181 tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata
     3241 atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg
     3301 gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac
     3361 cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt
     3421 tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg
     3481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg
     3781 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3841 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3901 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     3961 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4021 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4081 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4141 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4201 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4261 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4321 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4381 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4441 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4501 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4561 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4621 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4681 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4741 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4801 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4861 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4921 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4981 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5041 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5101 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5161 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5221 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5281 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5341 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5401 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5461 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5521 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5581 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5641 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5701 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5761 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5821 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     5881 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg
     5941 gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac
     6001 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     6061 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     6121 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     6181 gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca
     6241 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     6301 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     6361 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     6421 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     6481 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     6541 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     6601 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     6661 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     6721 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     6781 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     6841 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     6901 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     6961 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     7021 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     7081 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     7141 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     7201 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctga
//
