LOCUS       sequence-229648-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-27a-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-27a-5p
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..134)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(358..377)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    423..478
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(477..496)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 atcaaggagg ccgacaaaga gacatacgtc gagcagcacg aggtggctgt ggccagatac
       61 tgcgacctcc ctagcaaact ggggcacaaa cttaattgag aattctacca cccagctttc
      121 ttgtacaaag tggtacgtct caagaccaga ggtctctgct ttggtggatt gctcacaagc
      181 agctaagccc ttgctcacaa gcagctaagc ccttgctcac aagcagctaa gcccttgctc
      241 acaagcagct aagccctcaa ccagaagaga cctgtgcttc actcctcagg tgcaggctgc
      301 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
      361 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
      421 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
      481 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
      541 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
      601 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
      661 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
      721 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
      781 tgtccctctt ctcttatgga gatcccgagc ggtgcgtttt tatgcttgta gtattgtata
//
