LOCUS       sequence-22965-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  TITLE     Zebrafish Probe Plasmids
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZP44 Wnt8 5' orf2
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(613..629)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(613..629)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(666..686)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(666..684)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(705..721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(705..721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(718..740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    729..745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(753..759,760..777,778..783))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    798..819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(936..953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgacggtatc gataagcttg atatcgaatt tctaaacaat tcgtggatgc gcttgagact
       61 ggacaagacg cccgagctgc agttaatctt cataataatg aagctggacg tttggctgta
      121 aaagcgacaa tgaagaggat ttgtagatgt catggcatgt cagaaagttg cacaatgcag
      181 aattctctct ccaaaatcca cgttgtcgct acatcctccc catagccagc cacgaccccc
      241 aagtctgccg ttcctcgagc tgtcacaccc gcattcgttt agatctccaa gactgcagtt
      301 tctggttaaa gtgtacatta cgccggctgc gcttattgca tggacaaatg atgactctct
      361 tgttgcactt ctcaagcctt tgtgagtaga gagttgcaga gcggtgtccg ggcagttcca
      421 cctgtcccat gcaaactggt gtttgcactc atgtatccca ctctgtgccc caacccgcac
      481 actatttgca taggtaagat aagcctttgg cccagttatc agcaaattgt tcattgtcca
      541 agtgtgtcca aggatagctt tgtcccaaat agggaataga aaggcccaac ggcagaagta
      601 agccattatt tggatccact agttctagag cggccgccac cgcggtggag ctccagcttt
      661 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
      721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      781 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      961 gtcg
//
