LOCUS       sequence-22966-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Lekven AC, Thorpe CJ, Waxman JS, Moon RT
  TITLE     Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript
            and is required for mesoderm and neurectoderm patterning.
  JOURNAL   Dev Cell. 2001 Jul;1(1):103-14. doi: 10.1016/s1534-5807(01)00007-7.
  PUBMED    11703928
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Dev Cell"; date: "2001-07"; volume: "1"; issue: "1"; pages: 
            "103-14"
COMMENT     Sequence Label: ZP43 Wnt8 3' orf1
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(634..652)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(635..652)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(670..686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(670..686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(683..705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    694..710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcggccgcg ggaattcgat tgtctggaag ctgtagtatt cagacatgct ggatgcagct
       61 tgctgatttc agagatatcg gctcctacct aaaaatcaag catgatcaag cacggaagtt
      121 ggagatggat aaaattcgga tgagggcagg caatagcgcg gacaaccgtg gcgcaatagc
      181 ggacacgttc agcgcagttg cgcgcacaga actcattttt atggaagatt cacctgatta
      241 ctgcgtgaaa aacctcagca tgggactaca tggaactgaa ggcagggaat gtctacaaag
      301 cgggaagaat ctttctcaat gggagagaag aagctgcagg cggctatgcc acgaatgtgg
      361 cctgaaagta gaagagagga ggattgagac agtgagcagc tgtaactgta aatttcactg
      421 gtgctgcaca gtcaaatgcg aaacatgcac tcaaaccgtt accaggtatt tttgcgcaaa
      481 aaggcacaga aatcgccggc cgcacaacca ttcacgcaaa agacaacaca cgcgtcgtgg
      541 atgaagtaat cctaatcact agtgaattcg cggccgcctg caggtcgacc atatgggaga
      601 gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc
      661 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      721 cata
//
