LOCUS       sequence-229670-        9932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on nuclear body formation and 
            splicing of full-length TDP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1147 GFP-TDP43
FEATURES             Location/Qualifiers
     source          1..9932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    225..341
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        364..667
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        668..871
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     822..842
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     868..892
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      982..991
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2955..2979)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3015..3603
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3068..3088)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3486..3497)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3617..4116
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3652..3675)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4049..4068
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4137..4736
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4137..4156)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4633..4653
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4874..5107
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5179..5300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5216..5235)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5270..5289
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5340..5475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5495..5514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5496..5514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5524..5541)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5524..5540)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5533..5555)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5682..6137
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5769..5788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5979..6000
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6163..6267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6268..6336,6337..7128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6486..6505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7299..7887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7788..7807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8041..8058
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8175..8196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8211..8216,8217..8234,8235..8241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8249..8265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8254..8276
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8273..8289
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8273..8289
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8308..8328
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8310..8328
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8356..8582
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8583..8763
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8810..8935
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9428..9661
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9846..9890
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
       61 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
      121 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
      181 agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg
      241 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
      301 actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag cttgggagtt
      361 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      421 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      481 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      541 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      601 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      661 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      721 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      781 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      841 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
      901 acgccatcca cgctgttttg acctccatag aagacaccga ctctagtcca gtgtggtgga
      961 attctgcaga ttaccaccgg tgccaccatg ggaagtaaag gagaggaact atttacaggt
     1021 actgtgccta tcaaagtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgcgt
     1081 ggcgagggcg agggcgatgc caccgagggc aagctgaccc tgaagttcat ctgcaccacc
     1141 ggcaagctgc cggtgccgtg gccgaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1201 ttcagccgct acccggacca catgaagcgt cacgacttct tcaagtccgc catgccggaa
     1261 ggctacgtcc aggagcgcac catcgagttc aaggacgacg gcacctacaa gacccgcgcg
     1321 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg caacgacttc
     1381 aaggaggacg gcaacatcct gggtcacaag ctggagtaca accacaacag ccacaacgtc
     1441 cgtatcgaag ccgacaagca gaagaacggc atcaaggcta acttcaagat ccgccacaac
     1501 gttgaggacg gcagccagca ggaagccgac cacaaacagc agaacacccc gatcggcgac
     1561 ggcccggtgc gcctgccgga caaccactat ctgagcaccc agaccacgct gagcaaagac
     1621 ccgaacgaga agcgcgatca catggtcctg aaggagttcg tgacggcagc cggtatcact
     1681 aaaggtgaag atgaaagtgg atccatgtct gaatatattc gggtaaccga agatgagaac
     1741 gatgagccca ttgaaatacc atcggaagac gatgggacgg tgctgctctc cacggttaca
     1801 gcccagtttc caggggcgtg tgggcttcgc tacaggaatc cagtgtctca gtgtatgaga
     1861 ggtgtccggc tggtagaagg aattctgcat gccccagatg ctggctgggg aaatctggtg
     1921 tatgttgtca actatccaaa agataacaaa agaaaaatgg atgagacaga tgcttcatca
     1981 gcagtgaaag tgaaaagagc agtccagaaa acatccgatt taatagtgtt gggtctccca
     2041 tggaaaacaa ccgaacagga cctgaaagag tattttagta cctttggaga agttcttatg
     2101 gtgcaggtca agaaagatct taagactggt cattcaaagg ggtttggctt tgttcgtttt
     2161 acggaatatg aaacacaagt gaaagtaatg tcacagcgac atatgataga tggacgatgg
     2221 tgtgactgca aacttcctaa ttctaagcaa agccaagatg agcctttgag aagcagaaaa
     2281 gtgtttgtgg ggcgctgtac agaggacatg actgaggatg agctgcggga gttcttctct
     2341 cagtacgggg atgtgatgga tgtcttcatc cccaagccat tcagggcctt tgcctttgtt
     2401 acatttgcag atgatcagat tgcgcagtct ctttgtggag aggacttgat cattaaagga
     2461 atcagcgttc atatatccaa tgccgaacct aagcacaata gcaatagaca gttagaaaga
     2521 agtggaagat ttggtggtaa tccaggtggc tttgggaatc agggtggatt tggtaatagc
     2581 agagggggtg gagctggttt gggaaacaat caaggtagta atatgggtgg tgggatgaac
     2641 tttggtgcgt tcagcattaa tccagccatg atggctgccg cccaggcagc actacagagc
     2701 agttggggta tgatgggcat gttagccagc cagcagaacc agtcaggccc atcgggtaat
     2761 aaccaaaacc aaggcaacat gcagagggag ccaaaccagg ccttcggttc tggaaataac
     2821 tcttatagtg gctctaattc tggtgcagca attggttggg gatcagcatc caatgcaggg
     2881 tcgggcagtg gttttaatgg aggctttggc tcaagcatgg attctaagtc ttctggctgg
     2941 ggaatgtaga ctagacccag ctttcttgta caaagtggtt gatcctctat ccagcacagt
     3001 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3061 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3121 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3181 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3241 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3301 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3361 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     3421 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3481 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3541 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3601 cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     3661 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     3721 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     3781 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     3841 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     3901 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     3961 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     4021 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     4081 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     4141 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     4201 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     4261 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4321 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     4381 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     4441 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     4501 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     4561 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     4621 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     4681 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     4741 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc
     4801 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4861 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
     4921 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4981 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5041 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5101 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5161 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5221 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5281 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     5341 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5401 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5461 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5521 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5581 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5641 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     5701 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5761 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5821 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5881 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5941 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6001 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6061 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6121 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6241 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6301 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6361 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6421 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6481 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6541 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6601 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6661 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6721 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6781 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6841 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6901 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6961 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7021 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7081 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7141 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7441 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7921 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7981 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8041 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8101 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8161 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8221 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8281 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     8341 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     8401 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     8461 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     8521 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     8581 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8641 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8701 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8761 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     8821 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     8881 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     8941 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     9001 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     9061 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     9121 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     9181 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     9241 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     9301 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     9361 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     9421 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     9481 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     9541 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     9601 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9661 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9721 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9781 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9841 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9901 ttaacataac aaattggctg tggtatataa aa
//
