LOCUS       sequence-229680-        9927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on nuclear body formation and 
            splicing of full-length TDP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1412 GFP-TDP43 FYW-L
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    208..324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        347..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     805..825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     851..875
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      965..974
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2933..2957)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2993..3581
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3046..3066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3464..3475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3595..4094
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3630..3653)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4027..4046
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4115..4714
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4115..4134)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4611..4631
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4852..5085
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5157..5278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5194..5213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5248..5267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5318..5453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5473..5492)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5474..5492)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5502..5519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5502..5518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5511..5533)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5660..6115
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5747..5766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5957..5978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6141..6245
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6246..6314,6315..7106)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6464..6483)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7277..7865
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7766..7785
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8019..8036
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8153..8174
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8189..8194,8195..8212,8213..8219)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8227..8243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8232..8254
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8251..8267
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8251..8267
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8286..8306
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8288..8306
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8334..8560
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8561..8741
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8788..8913
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9406..9639
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9824..9868
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
       61 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
      121 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
      181 gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag
      241 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      301 acaaattaca aaattcaaaa ttttatcgat aagcttggga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      901 ttgacctcca tagaagacac cgactctagt ccagtgtggt ggaattctgc agattaccac
      961 cggtgccacc atgggaagta aaggagagga actatttaca ggtactgtgc ctatcaaagt
     1021 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg cgtggcgagg gcgagggcga
     1081 tgccaccgag ggcaagctga ccctgaagtt catctgcacc accggcaagc tgccggtgcc
     1141 gtggccgacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctacccgga
     1201 ccacatgaag cgtcacgact tcttcaagtc cgccatgccg gaaggctacg tccaggagcg
     1261 caccatcgag ttcaaggacg acggcaccta caagacccgc gcggaggtga agttcgaggg
     1321 cgacaccctg gtgaaccgca tcgagctgaa gggcaacgac ttcaaggagg acggcaacat
     1381 cctgggtcac aagctggagt acaaccacaa cagccacaac gtccgtatcg aagccgacaa
     1441 gcagaagaac ggcatcaagg ctaacttcaa gatccgccac aacgttgagg acggcagcca
     1501 gcaggaagcc gaccacaaac agcagaacac cccgatcggc gacggcccgg tgcgcctgcc
     1561 ggacaaccac tatctgagca cccagaccac gctgagcaaa gacccgaacg agaagcgcga
     1621 tcacatggtc ctgaaggagt tcgtgacggc agccggtatc actaaaggtg aagatgaaag
     1681 tggatccatg tctgaatata ttcgggtaac cgaagatgag aacgatgagc ccattgaaat
     1741 accatcggaa gacgatggga cggtgctgct ctccacggtt acagcccagt ttccaggggc
     1801 gtgtgggctt cgctacagga atccagtgtc tcagtgtatg agaggtgtcc ggctggtaga
     1861 aggaattctg catgccccag atgctggctg gggaaatctg gtgtatgttg tcaactatcc
     1921 aaaagataac aaaagaaaaa tggatgagac agatgcttca tcagcagtga aagtgaaaag
     1981 agcagtccag aaaacatccg atttaatagt gttgggtctc ccatggaaaa caaccgaaca
     2041 ggacctgaaa gagtatttta gtacctttgg agaagttctt atggtgcagg tcaagaaaga
     2101 tcttaagact ggtcattcaa aggggtttgg ctttgttcgt tttacggaat atgaaacaca
     2161 agtgaaagta atgtcacagc gacatatgat agatggacga tggtgtgact gcaaacttcc
     2221 taattctaag caaagccaag atgagccttt gagaagcaga aaagtgtttg tggggcgctg
     2281 tacagaggac atgactgagg atgagctgcg ggagttcttc tctcagtacg gggatgtgat
     2341 ggatgtcttc atccccaagc cattcagggc ctttgccttt gttacatttg cagatgatca
     2401 gattgcgcag tctctttgtg gagaggactt gatcattaaa ggaatcagcg ttcatatatc
     2461 caatgccgaa cctaagcaca actcaaaccg gcagctggaa cgctcaggcc gcctgggagg
     2521 taatcctgga ggactcggta accaaggtgg cctcggaaac agcaggggag gcggtgcagg
     2581 actgggcaac aatcaaggga gtaacatggg tgggggcatg aaccttggcg ctcttagcat
     2641 caaccccgcg atgatggcgg cagctcaggc tgcccttcaa agtagttggg gtatgatggg
     2701 tatgctggcg agccagcaga accagagcgg cccctccggg aacaaccaaa accagggtaa
     2761 catgcagcgc gaaccgaatc aggctctggg gtccggtaac aactccctgt ccggtagtaa
     2821 ttcaggagct gctattggtc tggggtcagc cagtaatgca ggaagcgggt ctggcctgaa
     2881 tggggggctg ggaagtagca tggatagtaa gtcttcagga cttggaatgt agacccagct
     2941 ttcttgtaca aagtggttga tcctctatcc agcacagtgg cggccgctcg acaatcaacc
     3001 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3061 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3121 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3181 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3241 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3301 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3361 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     3421 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3481 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3541 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc taccgggtag
     3601 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     3661 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     3721 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     3781 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     3841 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     3901 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     3961 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     4021 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     4081 ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg
     4141 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4201 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4261 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4321 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4381 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4441 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4501 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4561 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     4621 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     4681 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     4741 gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga
     4801 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4861 tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     5101 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     5161 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5221 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5281 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5341 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5401 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     5461 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     5521 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     5581 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     5641 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5701 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5761 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5821 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5881 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5941 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6001 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6061 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     6121 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6181 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6241 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6301 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6361 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6421 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6481 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6541 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6601 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6661 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6721 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6781 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6841 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6901 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6961 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7021 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7081 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7141 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7201 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7261 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7321 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7381 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7441 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7501 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7561 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7621 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7681 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7741 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7801 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7861 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7921 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7981 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8041 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8101 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8161 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     8221 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     8281 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     8341 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     8401 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     8461 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     8521 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     8581 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8641 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8701 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8761 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8821 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8881 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8941 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     9001 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     9061 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     9121 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     9181 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     9241 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     9301 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     9361 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     9421 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     9481 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     9541 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     9601 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     9661 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     9721 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9781 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9841 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9901 gtatataaaa ttattcataa tgatagt
//
