LOCUS       sequence-229683-        9927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on nuclear body formation and 
            splicing of full-length TDP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1413 GFP-TDP43 VLIM-F
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    161..277
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        300..603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        604..807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     758..778
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     804..828
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      918..927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2886..2910)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2946..3534
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2999..3019)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3417..3428)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3548..4047
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3583..3606)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3980..3999
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4068..4667
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4068..4087)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4564..4584
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4805..5038
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5110..5231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5147..5166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5201..5220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5271..5406
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5426..5445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5427..5445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5455..5472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5455..5471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5464..5486)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5613..6068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5700..5719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5910..5931
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6094..6198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6199..6267,6268..7059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6417..6436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7230..7818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7719..7738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7972..7989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8106..8127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8142..8147,8148..8165,8166..8172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8180..8196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8185..8207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8204..8220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8204..8220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8239..8259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8241..8259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8287..8513
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8514..8694
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8741..8866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9359..9592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9777..9821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
       61 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
      121 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga
      181 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      241 aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg ggagttccgc
      301 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      361 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      421 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      481 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      541 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      601 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      661 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      721 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      781 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      841 catccacgct gttttgacct ccatagaaga caccgactct agtccagtgt ggtggaattc
      901 tgcagattac caccggtgcc accatgggaa gtaaaggaga ggaactattt acaggtactg
      961 tgcctatcaa agtcgagctg gacggcgacg taaacggcca caagttcagc gtgcgtggcg
     1021 agggcgaggg cgatgccacc gagggcaagc tgaccctgaa gttcatctgc accaccggca
     1081 agctgccggt gccgtggccg accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1141 gccgctaccc ggaccacatg aagcgtcacg acttcttcaa gtccgccatg ccggaaggct
     1201 acgtccagga gcgcaccatc gagttcaagg acgacggcac ctacaagacc cgcgcggagg
     1261 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcaac gacttcaagg
     1321 aggacggcaa catcctgggt cacaagctgg agtacaacca caacagccac aacgtccgta
     1381 tcgaagccga caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg
     1441 aggacggcag ccagcaggaa gccgaccaca aacagcagaa caccccgatc ggcgacggcc
     1501 cggtgcgcct gccggacaac cactatctga gcacccagac cacgctgagc aaagacccga
     1561 acgagaagcg cgatcacatg gtcctgaagg agttcgtgac ggcagccggt atcactaaag
     1621 gtgaagatga aagtggatcc atgtctgaat atattcgggt aaccgaagat gagaacgatg
     1681 agcccattga aataccatcg gaagacgatg ggacggtgct gctctccacg gttacagccc
     1741 agtttccagg ggcgtgtggg cttcgctaca ggaatccagt gtctcagtgt atgagaggtg
     1801 tccggctggt agaaggaatt ctgcatgccc cagatgctgg ctggggaaat ctggtgtatg
     1861 ttgtcaacta tccaaaagat aacaaaagaa aaatggatga gacagatgct tcatcagcag
     1921 tgaaagtgaa aagagcagtc cagaaaacat ccgatttaat agtgttgggt ctcccatgga
     1981 aaacaaccga acaggacctg aaagagtatt ttagtacctt tggagaagtt cttatggtgc
     2041 aggtcaagaa agatcttaag actggtcatt caaaggggtt tggctttgtt cgttttacgg
     2101 aatatgaaac acaagtgaaa gtaatgtcac agcgacatat gatagatgga cgatggtgtg
     2161 actgcaaact tcctaattct aagcaaagcc aagatgagcc tttgagaagc agaaaagtgt
     2221 ttgtggggcg ctgtacagag gacatgactg aggatgagct gcgggagttc ttctctcagt
     2281 acggggatgt gatggatgtc ttcatcccca agccattcag ggcctttgcc tttgttacat
     2341 ttgcagatga tcagattgcg cagtctcttt gtggagagga cttgatcatt aaaggaatca
     2401 gcgttcatat atccaatgcc gaacctaagc ataacagtaa tcggcaattt gagcgatccg
     2461 gcagatttgg agggaaccct ggaggattcg gcaaccaggg gggctttgga aattcaagag
     2521 gagggggtgc gggattcggt aacaaccagg ggtccaattt cggtgggggg ttcaactttg
     2581 gggcgttttc tttcaatccc gctatgatgg ctgcggctca agcggctctg caaagtagct
     2641 ggggcatgat gggtatgctc gcgtcacaac agaatcagag tggaccaagt ggaaataatc
     2701 agaatcaagg gaattttcaa cgagaaccta accaagcatt cggatctggt aataatagct
     2761 actccgggag taactccgga gcagctttcg ggtggggctc cgcgtcaaac gctggcagcg
     2821 gctctggatt caacggggga ttcggaagca gtttcgattc aaaaagcagt ggctgggggt
     2881 tctagaccca gctttcttgt acaaagtggt tgatcctcta tccagcacag tggcggccgc
     2941 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3001 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3061 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3121 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3181 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3241 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3301 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     3361 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3421 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3481 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     3541 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     3601 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     3661 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     3721 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     3781 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     3841 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     3901 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     3961 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     4021 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca
     4081 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     4141 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     4201 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     4261 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     4321 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     4381 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     4441 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     4501 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     4561 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     4621 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     4681 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atctcgaggg cccggtacct
     4741 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     4801 ggactggaag ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     4861 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4921 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4981 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5041 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     5101 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5161 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5221 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     5281 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5341 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5401 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     5461 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5521 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5581 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     5641 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5701 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5761 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5821 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5881 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5941 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6001 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     6061 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6421 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6901 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6961 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7021 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7081 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7141 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7621 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7801 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7861 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7921 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7981 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8041 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8101 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8161 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8221 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     8281 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     8341 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     8401 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     8461 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     8521 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8581 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8641 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8701 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8761 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8821 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8881 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8941 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     9001 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     9061 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     9121 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     9181 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     9241 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     9301 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     9361 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     9421 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     9481 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     9541 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     9601 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     9661 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     9721 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9781 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9841 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9901 gaatagtttt tgctgtactt tctatag
//
