LOCUS       sequence-229690-       10356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Foxo1 from the TRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, 
            Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
  TITLE     CRISPR Activation Screens Systematically Identify Factors that Drive
            Neuronal Fate and Reprogramming.
  JOURNAL   Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003.
  PUBMED    30318302
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003."
COMMENT     Sequence Label: TetO-FUW-Foxo1
FEATURES             Location/Qualifiers
     source          1..10356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2724
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2828..2837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4817..5405
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4870..4890)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5288..5299)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5408..5424)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5409..5425)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5930..6110
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6136..6153)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6142..6366
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6412..6840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6499..6518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6709..6730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6849..6869)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6854..7183
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7034..7169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7096..7115
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7231..7278
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7297..7671
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7801..7922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7838..7857)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7892..7911
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7971..7987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7971..7987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7984..8006)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7995..8011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8019..8025,8026..8043,8044..8049))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8064..8085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8202..8219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8373..8961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8453..8472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9132..9923,9924..9992))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9755..9774
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9993..10097)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10172..10191)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
     2821 ttcgctagcc accatggccg aagcgcccca ggtggtggag accgacccgg acttcgagcc
     2881 gctgccccgg cagcgctcct gtacctggcc gctgcccagg ccggagttta accagtccaa
     2941 ctcgaccacc tccagtccgg cgccgtcggg cggcgcggcc gccaaccccg acgccgcggc
     3001 gagcctggcc tcggcgtccg ctgtcagcac cgactttatg agcaacctga gcctgctgga
     3061 ggagagtgag gacttcgcgc gggcgccagg ctgcgtggcc gtggcggcgg cggctgcggc
     3121 cagcaggggc ctgtgcgggg acttccaggg ccccgaggcg ggctgcgtgc acccagcgcc
     3181 gccacagccc ccaccgaccg ggccgctgtc gcagccccca cccgtgcctc cctccgctgc
     3241 cgccgccgcg gggccactcg cgggacagcc gcgcaagacc agctcgtcgc gccgcaacgc
     3301 gtggggcaac ctgtcgtacg ccgacctcat caccaaggcc atcgagagct cagccgagaa
     3361 gaggctcacc ctgtcgcaga tctacgagtg gatggtgaag agcgtgccct acttcaagga
     3421 taagggcgac agcaacagct cggcgggctg gaagaattca attcgccaca atctgtccct
     3481 tcacagcaag tttattcgag tgcagaatga aggaactgga aagagttctt ggtggatgct
     3541 caatccagag ggaggcaaga gcggaaaatc accccggaga agagctgcgt ccatggacaa
     3601 caacagtaaa tttgctaaga gccgagggcg ggctgctaag aaaaaagcat ctctccagtc
     3661 tgggcaagag ggtcctggag acagccctgg gtctcagttt tctaagtggc ctgcgagtcc
     3721 tgggtcccac agcaacgatg actttgataa ctggagtaca tttcgtcctc gaaccagctc
     3781 aaatgctagt accatcagtg ggagactttc tcccatcatg acagagcagg atgacctggg
     3841 agatggggac gtgcattccc tggtgtatcc accctctgct gccaagatgg cgtctacgct
     3901 gcccagtctg tctgaaatca gcaatccaga aaacatggag aaccttctgg ataatctcaa
     3961 ccttctctcg tccccaacat ctttaactgt gtccacccag tcctcgcctg gcagcatgat
     4021 gcagcagaca ccatgctatt cgtttgcacc gccaaacacc agtctaaatt cacccagtcc
     4081 aaactactca aagtacacat acggccaatc cagcatgagc cctttgcccc agatgcctat
     4141 gcagacactt caggacagca aatcaagtta cggaggattg aaccagtata actgtgcccc
     4201 aggactcttg aaagagttgt tgacttctga ctctcctccc cacaatgaca ttatgtcacc
     4261 ggttgatccc ggagtggccc aacccaacag tcgggtcctg ggccaaaatg taatgatggg
     4321 ccctaattcg gtcatgccag cgtatggcag ccaggcatct cataacaaaa tgatgaaccc
     4381 cagctcccac acccaccctg gacatgcaca gcaaacggct tcggtcaacg gccgtaccct
     4441 gccccatgtg gtgaacacca tgcctcacac atctgccatg aaccgcttga cccccgtgaa
     4501 gacaccttta caagtgcctc tgtcccaccc catgcagatg agtgccctgg gcagctactc
     4561 ctcggtgagc agctgcaatg gctatggtag gatgggtgtc ctccaccagg agaagctccc
     4621 aagtgacttg gatggcatgt ttattgagcg cttggactgt gacatggagt ccatcattcg
     4681 gaatgacctc atggatggag ataccttgga ttttaacttt gataatgtgt tgcccaacca
     4741 aagcttccca cacagtgtca agactacaac acacagctgg gtgtcaggct aggaattcga
     4801 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4861 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4921 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4981 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5041 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5101 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5161 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     5221 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5281 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5341 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5401 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     5461 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     5521 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     5581 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5641 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     5701 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     5761 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     5821 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     5881 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     5941 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6001 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6061 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     6121 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6181 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6241 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6301 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6361 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     6421 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6481 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6541 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6601 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6661 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6721 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6781 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6841 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     6901 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6961 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7021 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7081 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7141 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7201 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     7261 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     7321 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     7381 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     7441 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     7501 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     7561 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     7621 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     7681 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7741 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7801 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7861 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7921 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7981 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8041 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8101 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8161 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9721 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    10141 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    10201 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
    10261 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
    10321 gttttgcgct gcttcgcgat gtacgggcca gatata
//
