LOCUS       sequence-229721-        3708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible donor vector with actin-G168D,Y169D..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Viita T, Kyheroinen S, Prajapati B, Virtanen J, Frilander MJ, 
            Varjosalo M, Vartiainen MK
  TITLE     Nuclear actin interactome analysis links actin to KAT14 histone 
            acetyl transferase and mRNA splicing.
  JOURNAL   J Cell Sci. 2019 Apr 17;132(8). pii: jcs.226852. doi: 
            10.1242/jcs.226852.
  PUBMED    30890647
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-04-17"; volume: "132(8). pii: jcs.226852. doi"; 
            pages: " 10.1242/jcs.226852"
COMMENT     Sequence Label: pENTR-actin-G168D,Y169D
FEATURES             Location/Qualifiers
     source          1..3708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1833..1932)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1949..1968)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1950..1968)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1973..1989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1973..1989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2091..2110)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2102..2911
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2169..2188)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3058..3646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3547..3566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat ggatgatgat atcgccgcgc tcgtcgtcga
      721 caacggctcc ggcatgtgca aggccggctt cgcgggcgac gatgcccccc gggccgtctt
      781 cccctccatc gtggggcgcc ccaggcacca gggcgtgatg gtgggcatgg gtcagaagga
      841 ttcctatgtg ggcgacgagg cccagagcaa gagaggcatc ctcaccctga agtaccccat
      901 cgagcacggc atcgtcacca actgggacga catggagaaa atctggcacc acaccttcta
      961 caatgagctg cgtgtggctc ccgaggagca ccccgtgctg ctgaccgagg cccccctgaa
     1021 ccccaaggcc aaccgcgaga agatgaccca gatcatgttt gagaccttca acaccccagc
     1081 catgtacgtt gctatccagg ctgtgctatc cctgtacgcc tctggccgta ccactggcat
     1141 cgtgatggac tccggtgacg gggtcaccca cactgtgccc atctacgagg acgacgccct
     1201 cccccatgcc atcctgcgtc tggacctggc tggccgggac ctgactgact acctcatgaa
     1261 gatcctcacc gagcgcggct acagcttcac caccacggcc gagcgggaaa tcgtgcgtga
     1321 cattaaggag aagctgtgct acgtcgccct ggacttcgag caagagatgg ccacggctgc
     1381 ttccagctcc tccctggaga agagctacga gctgcctgac ggccaggtca tcaccattgg
     1441 caatgagcgg ttccgctgcc ctgaggcact cttccagcct tccttcctgg gcatggagtc
     1501 ctgtggcatc cacgaaacta ccttcaactc catcatgaag tgtgacgtgg acatccgcaa
     1561 agacctgtac gccaacacag tgctgtctgg cggcaccacc atgtaccctg gcattgccga
     1621 caggatgcag aaggagatca ctgccctggc acccagcaca atgaagatca agatcattgc
     1681 tcctcctgag cgcaagtact ccgtgtggat cggcggctcc atcctggcct cgctgtccac
     1741 cttccagcag atgtggatca gcaagcagga gtatgacgag tccggcccct ccatcgtcca
     1801 ccgcaaatgc ttctagaagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat
     1861 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1921 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     1981 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     2041 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     2101 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     2161 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     2221 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     2281 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     2341 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     2401 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     2461 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     2521 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     2581 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     2641 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2701 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2761 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     2821 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2881 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2941 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     3001 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3061 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3181 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
