LOCUS       sequence-229729-       12940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli-C. difficile shuttle vector for CRISPR interference 
            (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt 
            Pgdh::sgRNA-rfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12940)
  AUTHORS   Muh U, Pannullo AG, Weiss DS, Ellermeier CD
  TITLE     A xylose-inducible expression system and a CRISPRi-plasmid for 
            targeted knock-down of gene expression in Clostridioides difficile.
  JOURNAL   J Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 
            10.1128/JB.00711-18.
  PUBMED    30745377
REFERENCE   2  (bases 1 to 12940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 10.1128/JB.00711-18."
COMMENT     Sequence Label: pIA33
FEATURES             Location/Qualifiers
     source          1..12940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2272..2298,2299..2301,2302..4788,4789..4791,
                     4792..6378)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        7272..7347
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(7742..8113)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            8146..8255
                     /note="incP origin of transfer"
     primer_bind     complement(9451..9468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9622..10210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9702..9721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcttagggt attttttttg tttaattcca atattggttc aaactcctgt gtaatacttt
       61 tgacacttcc atctgtattg aattgacagt tgatatggta tctcattatt ggcttgttct
      121 tatgtttttc tatgtattta ttttttactt tcaaataatt tgtatttcca tttttagtta
      181 tgtttaacac actatcctta ttactcaata aaattatttt ttgttttaac atatgattta
      241 tagaatctaa aaattgatat tgacttataa tagtactttc tacctctcta gctttttcta
      301 gtgtttcaaa ttcttcaaac tctgctatat tgctatagta tagcttatca ttaataatat
      361 tatctgacaa ctttaattcc attttataaa acaattccaa gttatgtata aaatttttaa
      421 tgttacaaca tatagactta ttatttgttc ttatatggta tctccattta ctacttctag
      481 ctctcatgtt atatgcacaa ctactcccaa cataaacata tattgcttgt tctaactcat
      541 atttttgtaa aatttcaaca tcatatccag tcaaatcgta ctctctctta tatacaaatg
      601 gtttttctaa ttttttgttc aataaatctc ctcctatttc ttacttaatt tagctaaaaa
      661 tctatttata tttcacctcc tttttgactt taagcctacg aataccatat ttatgattat
      721 aacatcttct atttttccca aatccttaca tctcccctaa tttttagact taagggcgaa
      781 ttctgcatca agctagcata aaaataagaa gcctgcattt gcaggcttct tatttttatg
      841 gtacgtataa tttaaagggt tatggctgta tatagtcata acccttatat ttcattaatt
      901 aaagttaaat tttgtatatt caaaaagctc ttgatattta cggcagagcc accatataga
      961 gatgcctcag accctaatga tgaaatttct atatttattc ctttgttaaa agagctaact
     1021 aacatatctt ttgtaatctg taatatctca gggatatcac taattatttg gctatttaaa
     1081 tatattattt caggagcata agttgtaata gcattattta tagctattgt taagtaacta
     1141 caaaactcat gaataacttt tttggcattc tggttatctt cataatatag ttgctttact
     1201 atatcagaat ctattttagg aatattttct aaagaagaaa gctgttcaaa tacctttttt
     1261 tctgaacaat actgttctaa acagccacga tttccacaag gacatagttt accattaggc
     1321 atgatgatag tgtgaccaat ttctccactc ataccatttc tgccactata taatttatta
     1381 tttattataa tgcctgaacc aaatccactg tgaatactga gactaagtag tgagttatgt
     1441 acagttgaaa aagtattctc agctaaagct gttaagtttg cttcattttc tatgtgaata
     1501 ggaaagtcat actttttgct aaggatgcta tataagtcaa tctcattaag attgtaatat
     1561 ggagtaaaca atactttatt ttcacaagta atcccatgta tagccaaagt aagaccaatt
     1621 accttataag gagtgtctat tttggatatg ttataactat ttataatttc atctatcaat
     1681 tgtataacat tgtctttact tacttgtata tctgttaatt tcttagaatt tataatagtt
     1741 ccatctaagt aagatagaga agaaaatata tagtcgtatc caatgtccat acttaaagaa
     1801 atccctgcac atttattaaa tactaataat atgggttttc ttccgccact atgagtacta
     1861 tttccaattc ctatttcatg aactagagat tcatcaataa gttttttggt aatagcggat
     1921 atagttgctt tattcaatcc tatagtagaa gctatacttg ccctagaaat aggaccattt
     1981 tttataattt gttcaagtac caatctttca ttcatttctc gaatagtgta tttatcagta
     2041 accaatttga tattcctcct taaaataata ttgtaatact ttttacacaa aaataaaagg
     2101 ttattttgca ttgacaaaga taattaaata ttttattatt agttcataag ttagtttaat
     2161 atactaacaa aaataaagca agtaaaatat acctaaaata taaaaaaatt aggataggaa
     2221 aacgatagtt atgaagtggc attcaaggag ggtaaagagg agagtcgacg catggataaa
     2281 aaatatagta taggattagc aataggaaca aatagtgtag gatgggcagt aataactgat
     2341 gaatataaag taccaagtaa aaaatttaaa gtattaggaa atacagatag acatagtata
     2401 aaaaaaaatt taataggtgc attacttttt gatagtggtg aaacagcaga agcaacaaga
     2461 cttaaaagaa cagcaagaag aagatatact agaagaaaaa atagaatatg ttatttacaa
     2521 gaaatattta gtaatgaaat ggcaaaagta gatgatagtt tttttcatag attagaagaa
     2581 agttttttag tagaagaaga taaaaaacac gaaagacatc caatatttgg aaatatagta
     2641 gatgaagtag catatcatga aaaatatcca acaatatatc atttaagaaa aaaacttgta
     2701 gatagtacag ataaagcaga tttaagactt atatatcttg cattagcaca tatgataaaa
     2761 tttagaggac attttcttat agaaggtgat ttaaatccag ataatagtga tgtagataaa
     2821 ctttttatac aattagtaca aacatataat caattatttg aagaaaatcc aataaatgca
     2881 agtggtgtag atgcaaaagc aatattaagt gcaagattaa gtaaaagtag aagattagaa
     2941 aatcttatag cacaattacc tggagaaaaa aaaaatggat tatttggtaa tcttatagct
     3001 ttaagtttag gattaacacc aaattttaaa tcaaattttg atcttgcaga agatgcaaaa
     3061 cttcaattaa gtaaagatac atatgatgat gatttagata atttacttgc acaaataggt
     3121 gatcaatatg cagatttatt tcttgcagca aaaaatcttt ctgatgcaat acttttaagt
     3181 gatatactta gagtaaatac agaaataaca aaagcaccat tatctgcaag tatgataaaa
     3241 agatatgatg aacatcatca agatttaaca ttacttaaag cattagtaag acaacaatta
     3301 ccagaaaaat ataaagaaat tttttttgat caaagtaaaa atggatatgc aggatatata
     3361 gatggtggtg caagtcaaga agaattttat aaatttatta aaccaatatt agaaaaaatg
     3421 gatggaacag aagaattatt agtaaaactt aatagagaag atttacttag aaaacaaaga
     3481 acatttgata atggaagtat accacatcaa atacatttag gtgaattaca tgcaatatta
     3541 agaagacaag aagattttta tccatttctt aaagataata gagaaaaaat tgaaaaaata
     3601 cttacattta gaataccata ttatgtagga ccattagcaa gaggaaatag tagatttgct
     3661 tggatgacaa gaaaaagtga agaaacaata acaccatgga attttgaaga agtagtagat
     3721 aaaggagcat ctgcacaaag ttttatagaa agaatgacaa attttgataa aaatcttcca
     3781 aatgaaaaag tattaccaaa acatagttta ttatatgaat attttacagt atataatgaa
     3841 ttaacaaaag taaaatatgt aacagaaggt atgagaaaac cagcattttt aagtggtgaa
     3901 caaaaaaaag ctatagtaga tttattattt aaaacaaata gaaaagtaac agtaaaacaa
     3961 ttaaaagaag attattttaa aaaaattgaa tgttttgata gtgtagaaat atctggtgta
     4021 gaagatagat ttaatgcaag tttaggaaca tatcatgatt tacttaaaat aataaaggat
     4081 aaagattttt tagataatga agaaaatgaa gatattttag aagatatagt attaacatta
     4141 actttatttg aagatagaga aatgatagaa gaaagattaa aaacttatgc tcatttattt
     4201 gatgataaag taatgaaaca acttaaaaga agaagatata caggttgggg aagattaagt
     4261 agaaaactta taaatggaat aagagataaa caaagtggaa aaacaatatt agattttctt
     4321 aaaagtgatg gatttgcaaa tagaaatttt atgcaattaa tacatgatga tagtttaaca
     4381 tttaaagaag atattcaaaa agcacaagta agtggacaag gtgatagttt acatgaacat
     4441 atagcaaatt tagcaggatc tccagcaata aaaaaaggta tattacaaac agtaaaagta
     4501 gttgatgaat tagtaaaagt aatgggtaga cataaaccag aaaatatagt tatagaaatg
     4561 gcaagagaaa atcaaacaac acaaaaagga caaaaaaata gtagagaaag aatgaaaaga
     4621 atagaagaag gtataaaaga attaggaagt caaatactta aagaacatcc agtagaaaat
     4681 acacaacttc aaaatgaaaa actttattta tattatttac aaaatggaag agatatgtat
     4741 gtagatcaag aattagatat aaatagatta agtgattatg atgtagatgc aatagtacca
     4801 caaagttttc ttaaagatga ttctatagat aataaagtat taacaagatc tgataaaaat
     4861 agaggtaaaa gtgataatgt accaagtgaa gaagttgtaa aaaaaatgaa aaattattgg
     4921 agacaattac ttaatgcaaa acttataaca caaagaaaat ttgataatct tacaaaagca
     4981 gaaagaggtg gattatctga attagataaa gctggattta taaaaagaca attagtagaa
     5041 actagacaaa taacaaaaca tgtagcacaa atattagata gtagaatgaa tacaaaatat
     5101 gatgaaaatg ataagcttat aagagaagta aaagttataa cattaaaaag taaattagta
     5161 agtgatttta gaaaagattt tcaattttat aaagtaagag aaataaataa ttatcatcat
     5221 gcacatgatg catatcttaa tgcagtagta ggaacagcat taataaaaaa atatcctaaa
     5281 ttagaatctg aatttgtata tggagattat aaagtatatg atgtaagaaa aatgatagca
     5341 aaaagtgaac aagaaatagg aaaagcaaca gcaaagtact ttttttattc taatataatg
     5401 aattttttta agacagaaat aactttagca aatggtgaaa taagaaaaag acctttaata
     5461 gaaactaatg gtgaaacagg tgaaatagta tgggataaag gtagagattt tgcaacagta
     5521 agaaaagtat taagtatgcc acaagtaaat atagtaaaaa aaacagaagt acaaactgga
     5581 ggatttagta aagaaagtat acttccaaaa agaaattcag ataaacttat tgcaagaaaa
     5641 aaagattggg atccaaaaaa atatggtgga tttgatagtc caacagtagc atatagtgta
     5701 ttagtagtag caaaagtaga aaaaggtaaa tctaaaaaac ttaaaagtgt aaaagaatta
     5761 cttggaataa caataatgga aagaagtagt tttgaaaaaa atcctattga ttttttagaa
     5821 gcaaaaggat ataaagaagt taaaaaagat ttaataataa aacttccaaa atattcttta
     5881 tttgaattag aaaatggaag aaaaagaatg ttagcaagtg ctggtgaatt acaaaaaggt
     5941 aatgaattag cattaccatc taaatatgta aattttcttt atttagcaag tcattatgaa
     6001 aaacttaaag gatctcctga agataatgaa caaaaacaat tatttgtaga acaacataaa
     6061 cattatttag atgaaataat tgaacaaata agtgaatttt ctaaaagagt aatattagca
     6121 gacgcaaatt tagataaagt attatctgct tataataaac atagagataa accaataaga
     6181 gaacaagcag aaaatataat acatcttttt acattaacaa atttaggtgc accagcagca
     6241 tttaaatatt ttgatacaac aatagataga aaaagatata caagtacaaa agaagtatta
     6301 gacgcaacat taatacatca aagtataaca ggattatatg aaacaagaat agatttatct
     6361 caattaggtg gtgattaact cgagcaaaaa catcgtagaa atacggtgtt ttttgttacc
     6421 ctaagtttca taaaaataag aagcctgcaa atgcaggctt cttattttta tggtttaaac
     6481 ggttttagct gggatatcgg caaaacaagc aaatactact atagaagcag ttaaaagggc
     6541 tattttttat aatctggagc atagacaaga agttagagaa gcaattaaaa aagaaaatat
     6601 aagaaaaatt cttattattg ggacatcaaa aaaaatggtt aatcaaattg tagagagatt
     6661 gtcaattgga agggtctgta agtttataaa tatacaagat ataagtacta aatatgaaat
     6721 agaaatagca aaacaagcta gaaaagaagg aaatcatgta attcctgttc cagccgttga
     6781 gataaaaagt atggcaagtg gtctcagtat aaattctttg aagagacttt ttagaaaagg
     6841 aaataatagg aatatggtag ttttagaaaa aacaattata agaccaactt ttagttatat
     6901 aggaaagttt tatattagtg ctgatgtaat taaacagatt attgaatatg aagtatacaa
     6961 ctttggaagc attgatagaa taaataaaat caatatagag aattgcaata attttatgaa
     7021 tatttttgta agtataaata tcaatgattt agaaactata aaaaaaattg aagacataca
     7081 acagacaata aaaaagtcta ttgagaaaat gactttagtt aatgtacaaa aaatagatat
     7141 taacattcat aaaattaaaa aaataaactt cctccaatgt taaaaaataa actgagaaaa
     7201 tgatatacta atttgcaata aagtgtgcta taattaaact gtaaatggcc aaactttcag
     7261 tttagcggtc tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     7321 cttgaaaaag tggcaccgag tcggtgcttt ttttctatgg agaaatctag atcagcatga
     7381 tgtctgacta gcggccgcgg atcctataag ttttaataaa actttaaata gaaaaaggct
     7441 tctctcatga gaagtctttt ttatttaaaa taaatataaa ataaaataga ggctataaat
     7501 agcctctatt ttatgtgaga aatccctaaa taaaaagatg ccagtgtgct ggaattcgcc
     7561 cttaggctgc gctagggacc tctttagctc cttggaagct gtcagtagta ggggatcggt
     7621 cttgccttgc tcgtcggtga tgtacttcac cagctccgcg aagtcgctct tcttgatgga
     7681 gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa
     7741 gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct
     7801 tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg
     7861 ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc
     7921 gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga
     7981 cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc
     8041 ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg
     8101 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca
     8161 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
     8221 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
     8281 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
     8341 ccgaaaaaat cgctataatg accccgaagc aggaagggcg aattctgcag tgcagatctg
     8401 accggtctct gaaaatataa aaaccacaga ttgatactaa aaccttggtt gtgttgcttt
     8461 tcggggctta aatcaaggaa aaatccttgt tttaagcctt tcaaaaagaa acacaaggtc
     8521 tttgtactaa cctgtggtta tgtataaaat tgtagatttt agggtaacaa aaaacaccgt
     8581 atttctacga tgtttttgct taaatacttg tttttagtta cagacaaacc tgaagttaac
     8641 tatttatcaa ttcctgcaat tcgtttacaa aacggcaaat gtgaaatccg tcacatactg
     8701 cgtgatgaac ttgaattgcc aaaggaagta taattttgtt atcttcttta taatatttcc
     8761 ccatagtaaa aataggaatc aaataatcat atcctttctg caaattcaga ttaaagccat
     8821 cgaaggttga ccacggtatc atagatacat taaaaatgtt ttccggagca tttggctttc
     8881 cttccattct atgattgttt ccataccgtt gcgtatcact ttcataatct gctaaaaatg
     8941 atttaaagtc agacttacac tcagtccaaa ggctggaaaa tgtttcagta tcattgtgaa
     9001 atattgtata gcttggtatc atctcatcat atatccccaa ttcaccatct tgattgattg
     9061 ccgtcctaaa ctctgaatgg cggtttacaa tcattgcaat ataataaagc attgcaggat
     9121 atagtttcat tcccttttcc tttatttgtg tgatatccac tttaacggtc atgctgtagg
     9181 tacaaggtac acttgcaaag tagtggtcaa aatactcttt tctgttccaa ctatttttat
     9241 caattttttc aaataccatc taagttccct ctcaaattca agtttatcgc tctaatgaac
     9301 aaagatatta taccacattt ttgtgaattt ttcaacttgc ccacttcgac tgcactcccg
     9361 acttaataac ttcttgaaca cttgccgaaa aagaaaaact gccgggtacg tacccgggga
     9421 tcgatccccg gccgagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9721 cccttgaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9781 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10021 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    10081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10261 ctcacgttaa gggattttgg tcatgaaatg caagtttcta actaacatac atcatagtta
    10321 ctaaactatg gtaactataa ttttattaac tatattaagc actgattagt actataactc
    10381 aatataagca tatcccctgt atcgtaacta gagaaccaaa cgacggaaaa agcgatatag
    10441 ataagtttag atagtgcacc aattttttac ttttctattt tagaatctag catttccaat
    10501 gcttactaat actatttttg atttttaact tgttttaaaa aagcacctac taattaggat
    10561 tctagctcta tattctagtt atacattctc tatattctag ctctatattc tagttataca
    10621 ttctctatat tctagctcta tattctagtt atacattctc tatattctag ctctatattc
    10681 tagttataca ttctctatat tctagctcta tattctagtt atacattctc tatattctag
    10741 ctctatattc tagttataca ttctctatat tctagctcta tattctagtt atacattctc
    10801 taaaaaacat atatatatat actacatata tatgtacaat tattttacca aaagaaattt
    10861 ttaaaacggc ttaatttcaa tactttgatg ccattttttt tttgcaattc tcgtacaatt
    10921 attttaccaa aagaaatttt tattaattag tttttttgca taaaaatagg actagaaaat
    10981 caattctaat cctatcaaaa atctacttgt ttaatctata tgtatagtac ataaatcctc
    11041 tatcatttat tttagtttcc catacatcta aaattttagt atctaataaa gattctatca
    11101 tatttgaaat tctttgctgt gttactccta aatcttttgc taattcctct tgattaattc
    11161 ttaatatatt tccttttatt ttatcatctt tgaccaataa atcttgctta tatctcatat
    11221 gagtatataa tcttaattct tcagttgaaa ttattcccca aattactgcc atggtaacaa
    11281 agtaactaat agtatattgt ttatctatat tacatctaac ttcttttaca ttgtatgtat
    11341 cttttattcc tagctttgta acaccttttg tttttgtaat ataatcatta tgttgaagtt
    11401 cttttaatgt ttctcttaat gtcttttcac tcataacaat gtttttaact ttcttcttac
    11461 gtttataggt tatcagctcc attatatcgt ctatgtttaa ttctctatct ttattgttaa
    11521 gtaacacatt atagataaac aattgattac cagtcattat tttaacccct ttcctattct
    11581 tatatttcaa atcctttgag tgcttatgtg gcatattgaa caaagtatct tcatcactat
    11641 atataaaatc tgattctatt ttattccaac aatcactatt aaactcacaa cctgtacaac
    11701 caaaattaac cgttttaagc ttctcataca tatacttgac ttgatattct agctcactat
    11761 ttgaaagact attttgtgat atatagttcc attctctagc aacggatatt acttgactta
    11821 aacttttatt cctcaatctt aaagtcacaa ctattttttg caaatctagg tttttatgtc
    11881 catctttact accatttttt aaaatatcta ctatacaatt tggtagctta ctatctaata
    11941 tatattttgt attagtatta cctactgttt ttacatcttt gcaatatttt ttagctaact
    12001 tttctatgtc atatctatat atatttttgt cttggtgtat tatttttact tgtttagtag
    12061 tatttttaat attatatgta taaggtactc ttaatacttg agtagataaa tttgcttgtt
    12121 tatctgcacc caatttattt attaatgtat ttgtaacttc tgatactaac ttaatgttat
    12181 tagttttatt aattagcaca taaacatgga atccatttcc actatcaact aggatatgat
    12241 aatgtaatcc tatcttagta aataaattaa ttatatcttt ataattaaaa ccttgtccta
    12301 attctttttt gtcaaaatca aatcctaatg catatctatt tattaagttt tcattggttc
    12361 ctgcttttcc atcagttgta cataggttaa aaaatgtatt ttcataataa atattttttg
    12421 cgaaggtaaa ttctactata tcatctatat ttttaaaaaa agttgtttta ctatattctt
    12481 taaattcttc tccgagttct aattgtttac tattactttt attattttga aatactctaa
    12541 catattcatt atcaataaga ttatttcctt gtcctatacc atctctaaat actgccatta
    12601 gaaatttttt caacttatat tttgaatcta attgttccat atatttatta tctccttatc
    12661 tagttttttt aagagataac actacattaa atatatactt attgactaat caacttttac
    12721 aactattgaa taattttagc ttagtatgta taataatatt gttaagatgc taaaattgat
    12781 agcaatatta ttatataact acagatactt aatcagttag accgatattg gagttggtag
    12841 ctccataaac tcaattggta gttgagttgt cattgtctag tagtattatc tcattttgtt
    12901 gttatcttaa aatattattt taaaaatact ctgcttggtc
//
