LOCUS       sequence-229745-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses recycling endosome-targeted recruiter protein in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FKBP-Rab11-Blasticidin
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..43
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     40..64
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(129..131,132..452)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     primer_bind     complement(622..640)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
       61 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctacta
      121 gaggatccat gggagtgcag gtggagacta tctccccagg agacgggcgc accttcccca
      181 agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga aagaaatttg
      241 attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag gaggtgatcc
      301 gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa ctgactatat
      361 ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca catgccactc
      421 tcgtcttcga tgtggagctt ctaaaactgg aaggtgctgc acttctgccc ggttctatgg
      481 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      541 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      601 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
      661 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
      721 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      781 tgaacttcga ggacggcggc g
//
