LOCUS       sequence-229753-        9817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged ERG protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Sandoval GJ, Pulice JL, Pakula H, Schenone M, Takeda DY, Pop M, 
            Boulay G, Williamson KE, McBride MJ, Pan J, St Pierre R, Hartman E, 
            Garraway LA, Carr SA, Rivera MN, Li Z, Ronco L, Hahn WC, Kadoch C
  TITLE     Binding of TMPRSS2-ERG to BAF Chromatin Remodeling Complexes 
            Mediates Prostate Oncogenesis.
  JOURNAL   Mol Cell. 2018 Aug 16;71(4):554-566.e7. doi: 
            10.1016/j.molcel.2018.06.040. Epub 2018 Aug 2.
  PUBMED    30078722
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.06.040"; journalName: "Mol Cell"; date: 
            "2018-08-16- 16"; volume: "71"; issue: "4"; pages: "554-566.e7"
COMMENT     Sequence Label: V5-ERG
FEATURES             Location/Qualifiers
     source          1..9817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        39..250
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          251..1189
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1146..1166
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1213..1222
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1222..1263
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        2656..3155
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2691..2714)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3088..3107
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3182..3580
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3604..4192
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3657..3677)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4075..4086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4399..5032
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5160..5176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5160..5176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5173..5195)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5184..5200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5208..5214,5215..5232,5233..5238))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5253..5274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5391..5408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5562..6150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5642..5661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6321..7112,7113..7181))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6944..6963
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7182..7286)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7334..7468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7371..7390)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7425..7444
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7636..8269
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8316..8441
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8938..9171
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9356..9400
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9663..9778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 atgaggccct ttcgtcttca ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac
       61 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
      121 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
      181 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
      241 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
      301 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
      361 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
      421 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
      481 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
      541 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
      601 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
      661 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
      721 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
      781 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
      841 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
      901 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
      961 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     1021 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     1081 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     1141 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     1201 atccgcggcc gcgccaccat gggtaagcct atccctaacc ctctcctcgg tctcgattct
     1261 acgacagaga tgaccgcgtc ctcctccagc gactatggac agacttccaa gatgagccca
     1321 cgcgtccctc agcaggattg gctgtctcaa cccccagcca gggtcaccat caaaatggaa
     1381 tgtaacccta gccaggtgaa tggctcaagg aactctcctg atgaatgcag tgtggccaaa
     1441 ggcgggaaga tggtgggcag cccagacacc gttgggatga actacggcag ctacatggag
     1501 gagaagcaca tgccaccccc aaacatgacc acgaacgagc gcagagttat cgtgccagca
     1561 gatcctacgc tatggagtac agaccatgtg cggcagtggc tggagtgggc ggtgaaagaa
     1621 tatggccttc cagacgtcaa catcttgtta ttccagaaca tcgatgggaa ggaactgtgc
     1681 aagatgacca aggacgactt ccagaggctc acccccagct acaatgccga catccttctc
     1741 tcacatctcc actacctcag agagactcct cttccacatt tgacttcaga tgatgttgat
     1801 aaagccttac aaaactctcc acggttaatg catgctagaa acacaggggg tgcagctttt
     1861 attttcccaa atacttcagt atatcctgaa gctacgcaaa gaattacaac taggccagat
     1921 ttaccatatg agccccccag gagatcagcc tggaccggtc acggccaccc cacgccccag
     1981 tcgaaagctg ctcaaccatc tccttccaca gtgcccaaaa ctgaagacca gcgtcctcag
     2041 ttagatcctt atcagattct tggaccaaca agtagccgcc ttgcaaatcc aggcagtggc
     2101 cagatccagc tttggcagtt cctcctggag ctcctgtcgg acagctccaa ctccagctgc
     2161 atcacctggg aaggcaccaa cggggagttc aagatgacgg atcccgacga ggtggcccgg
     2221 cgctggggag agcggaagag caaacccaac atgaactacg ataagctcag ccgcgccctc
     2281 cgttactact atgacaagaa catcatgacc aaggtccatg ggaagcgcta cgcctacaag
     2341 ttcgacttcc acgggatcgc ccaggccctc cagccccacc ccccggagtc atctctgtac
     2401 aagtacccct cagacctccc gtacatgggc tcctatcacg cccacccaca gaagatgaac
     2461 tttgtggcgc cccaccctcc agccctcccc gtgacatctt ccagtttttt tgctgcccca
     2521 aacccatact ggaattcacc aactgggggt atatacccca acactaggct ccccaccagc
     2581 catatgcctt ctcatctggg cacttactac taagcggccg cgccggctct agatcgcgaa
     2641 cgcgtgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     2701 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     2761 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     2821 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     2881 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     2941 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     3001 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     3061 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     3121 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttcctaggac
     3181 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     3241 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     3301 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     3361 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     3421 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     3481 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     3541 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa cccgggcggg gcgcgtctgg
     3601 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3661 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3721 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3781 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3841 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3901 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3961 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4021 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4081 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4141 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     4201 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     4261 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     4321 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4381 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     4441 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     4501 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     4561 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     4621 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     4681 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     4741 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     4801 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     4861 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4921 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4981 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     5041 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     5101 aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg
     5161 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5221 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5281 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5341 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5401 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5461 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5521 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5581 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5641 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5701 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5761 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5821 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5881 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5941 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6001 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6061 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6121 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6181 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6241 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6301 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6361 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6421 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6481 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6541 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6601 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6661 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6721 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6781 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6841 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6901 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6961 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7021 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7081 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7141 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7201 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7261 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     7321 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7381 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7441 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     7501 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     7561 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     7621 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     7681 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     7741 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     7801 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     7861 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     7921 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     7981 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     8041 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     8101 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8161 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8221 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8281 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8341 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8401 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8461 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8521 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8581 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8641 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8701 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8761 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     8821 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8881 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8941 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     9001 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     9061 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     9121 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     9181 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     9241 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9301 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9361 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9421 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9481 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9541 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     9601 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     9661 cctttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9721 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9781 gggtttatta cagggacagc agagatccag tttatcg
//
