LOCUS       sequence-229763-        3432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Pavicic M, Mouhu K, Wang F, Bilicka M, Chovancek E, Himanen K
  TITLE     Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin 
            E3 Ligases in Arabidopsis.
  JOURNAL   Front Plant Sci. 2017 Mar 28;8:416. doi: 10.3389/fpls.2017.00416. 
            eCollection 2017.
  PUBMED    28400782
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-03-28"; volume: "8:416. doi"; pages: " 
            10.3389/fpls.2017.00416. eCollection 2017"
COMMENT     Sequence Label: pDONR 221-PHL2 (NON-STOP)
FEATURES             Location/Qualifiers
     source          1..3432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1557..1656)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1673..1692)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1674..1692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1697..1713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1697..1713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1815..1834)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1826..2635
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1893..1912)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2782..3370
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3271..3290
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctct atgtactcag cgattcgctc gcttccactc gatggtggac acgttggtgg
      721 tgactaccat ggacctcttg acggaaccaa tcttcccggt gacgcttgtt tggttttaac
      781 gactgaccct aaacctcgtc tccggtggac aactgagctt catgagagat tcgttgacgc
      841 cgttactcag ctcggtggtc ctgacaaagc gactcccaaa actattatga gaacaatggg
      901 agtgaagggt ctcactctct accacctcaa atcacatctt cagaaattcc gcctagggag
      961 gcaagctggc aaagaatcaa ctgagaactc taaagatgct tcttgtgtag gggagagtca
     1021 ggacacaggt tcatcttcga catcatcaat gagaatggcg cagcaggagc agaacgaggg
     1081 ttaccaagtc accgaagctc tacgtgctca gatggaagtc caaagaagac tacacgatca
     1141 attggaggtg caacggaggc tccagctgag gatagaggca caaggaaaat acctgcaatc
     1201 gattcttgaa aaagcttgca aggcctttga cgagcaagct gctacttttg ctggacttga
     1261 ggctgctagg gaagagctat cagagctagc catcaaagtc tccaatagct ctcaaggaac
     1321 atcagtcccg tacttcgatg caacaaagat gatgatgatg ccatcgttgt cagagcttgc
     1381 agtagcaata gacaacaaaa acaacatcac aaccaactgt tcagtagaaa gctctctgac
     1441 ttccatcaca catgggagct ctatatctgc tgcatcaatg aagaagcgtc aacgtggaga
     1501 caatttgggc gtagggtatg aatcaggctg gattatgcct agtagcacca ttggaaaccc
     1561 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1621 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     1681 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     1741 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1801 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     1861 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     1921 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     1981 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     2041 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     2101 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     2161 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     2221 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     2281 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     2341 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     2401 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     2461 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     2521 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     2581 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     2641 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     2701 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     2761 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2821 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2881 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2941 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3001 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3061 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3121 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3181 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3241 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3301 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3361 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3421 tgctcacatg tt
//
