LOCUS       sequence-229770-        8578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a fusion of firefly luciferase and tandem Tomato red 
            fluorescent protein in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Patel MR, Chang YF, Chen IY, Bachmann MH, Yan X, Contag CH, Gambhir 
            SS
  TITLE     Longitudinal, noninvasive imaging of T-cell effector function and 
            proliferation in living subjects.
  JOURNAL   Cancer Res. 2010 Dec 15;70(24):10141-9.
  PUBMED    21159636
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2010-12-15"; volume: "70(24)"; pages: "10141-9"
COMMENT     Sequence Label: pcDNA3.1(+)/Luc2=tdT
FEATURES             Location/Qualifiers
     source          1..8578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     865..884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        865..883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             966..2615
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      2664..2673
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2670..4100
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(4171..4188)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4177..4401
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4447..4875
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4534..4553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4744..4765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4884..4904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4889..5218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5069..5204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5131..5150
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5285..6079
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5339..5358)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5949..5968
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6253..6374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6290..6309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6344..6363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6423..6439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6423..6439)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6436..6458)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6447..6463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6471..6477,6478..6495,6496..6501))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6516..6537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6654..6671)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6825..7413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6905..6924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7584..8375,8376..8444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8207..8226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8445..8549)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
      901 gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattca
      961 ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag
     1021 acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca
     1081 ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga
     1141 tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg
     1201 tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg
     1261 gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg
     1321 gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg
     1381 tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc
     1441 agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt
     1501 acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta
     1561 gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat
     1621 tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca
     1681 gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg
     1741 gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag
     1801 actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca
     1861 ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc
     1921 tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg
     1981 gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc
     2041 ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg
     2101 gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga
     2161 gcggctacgt taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc
     2221 acagcggcga catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga
     2281 agagcctgat caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc
     2341 tgcaacaccc caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg
     2401 agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg
     2461 tggactatgt ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg
     2521 tggacgaggt gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc
     2581 tcattaaggc caagaagggc ggcaagatcg ccgtgggaga tctaggaggt ggaggtagcg
     2641 gtggaggtgg aagccaggat cccgccacca tggtgagcaa gggcgaggag gtcatcaaag
     2701 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
     2761 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     2821 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
     2881 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
     2941 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
     3001 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
     3061 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     3121 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
     3181 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
     3241 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
     3301 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg
     3361 gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca
     3421 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
     3481 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     3541 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
     3601 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
     3661 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
     3721 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
     3781 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
     3841 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
     3901 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
     3961 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
     4021 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca
     4081 tggacgagct gtacaagtaa gaattctgca gatatccagc acagtggcgg ccgctcgagt
     4141 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     4201 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4261 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4321 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4381 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4441 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4501 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4561 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4621 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4681 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4741 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4801 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4861 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4921 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4981 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5041 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5101 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     5161 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5221 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     5281 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5341 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5401 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5461 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5521 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5581 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5641 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5701 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5761 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5821 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5881 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5941 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     6001 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     6061 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     6121 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6181 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6241 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6301 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6361 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6421 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6481 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6541 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6601 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6661 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6721 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7021 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7261 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7501 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7561 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7621 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7681 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7741 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7801 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7861 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7921 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7981 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8041 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8101 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8161 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8221 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8281 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8461 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8521 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacg
//
