LOCUS       sequence-229791-       11700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting 
            sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Pierce AJ, Hu P, Han M, Ellis N, Jasin M
  TITLE     Ku DNA end-binding protein modulates homologous repair of 
            double-strand breaks in mammalian cells.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3237-42.
  PUBMED    11751629
REFERENCE   2  (bases 1 to 11700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3237-42."
COMMENT     Sequence Label: pHPRT-DRGFP
FEATURES             Location/Qualifiers
     source          1..11700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     321..343
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     405..424
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             482..502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             524..544
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      758..767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(764..766,767..769,770..1483)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSG*G*QGNTYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     1417..1438
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1606..1727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1643..1662)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1697..1716
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(2388..2987)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2471..2491)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2968..2987
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3006..3503)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3054..3073)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3445..3468
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(3591..3610)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3656..3711
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3710..3729)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     CDS             4075..4095
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4117..4137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4351..4360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4357..4359,4360..4362,4363..4821)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(4877..4893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4901..4917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4925..4931,4932..4949,4950..4955))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4970..4991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      5096..5231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6990..7009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6991..7009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7016..7033)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7016..7032)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7025..7047)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7174..7629
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7261..7280)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7471..7492
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7655..7759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7760..7828,7829..8620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7978..7997)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8791..9379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9280..9299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9533..9550
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9667..9688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9703..9708,9709..9726,9727..9733)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9741..9757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9765..9781
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9802..9820
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9857..9873
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9857..9873
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     enhancer        11200..11579
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
       61 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
      121 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
      181 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
      241 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
      301 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
      361 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
      421 tgtgctgtct catcattttg gcaaagaatt cagatccgcc gccactatgg gatcaagatc
      481 gccaaaaaag aagagaaagg tgccgaagaa gcatgcagca ccaccaaaaa aaaaacgaaa
      541 agtagaagac ccacgaggca acaccagcgg cgtgctgagc acccccaagg ccaagagggc
      601 caagcacccc cccggcaccg agaagcccag gagcaggagc cagagcgagc agcccgccac
      661 ctgccccatc tgctacgccg tgatcaggca gagcaggaac ctgaggaggc acctggagct
      721 gaggcacttc gccaagcccg gcgtggatcc accggtcgcc accatggtga gcaagggcga
      781 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      841 caagttcagc gtgtccggct agggataaca gggtaatacc tacggcaagc tgaccctgaa
      901 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      961 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1021 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1081 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1141 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1201 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1261 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1321 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1381 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1441 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     1501 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1561 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1621 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1681 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     1741 aagcgttaat ctgaagcttc tgatggaatt agaacttggc aaaacaatac tgagaatgaa
     1801 gtgtatgtgg aacagaggct gctgatctcg ttcttcaggc tatgaaactg acacatttgg
     1861 aaaccacagt acttagaacc acaaagtggg aatcaagaga aaaacaatga tcccacgaga
     1921 gatctataga tctatagatc atgagtggga ggaatgagct ggcccttaat ttggttttgc
     1981 ttgtttaaat tatgatatcc aactatgaaa cattatcata aagcaatagt aaagagcctt
     2041 cagtaaagag caggcattta tctaatccca ccccaccccc acccccgtag ctccaatcct
     2101 tccattcaaa atgtaggtac tctgttctca cccttcttaa caaagtatga caggaaaaac
     2161 ttccatttta gtggacatct ttattgttta atagatcatc aatttcgatc cgctcctggg
     2221 caccgaactg cgccgcgtgt tcagcagggt cggcgtgttc ggtgtgtccc ccgcggtggg
     2281 cctcgggggc gggtgcgggg tcggcggggc cgccccgggt ggcttcggtc ggagccatgg
     2341 ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc
     2401 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
     2461 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
     2521 accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
     2581 ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg
     2641 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
     2701 gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
     2761 cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
     2821 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cgggtcgacg
     2881 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg
     2941 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt ggcggctgga
     3001 tcggtcgaaa ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcgcttttga
     3061 agcgtgcaga atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg
     3121 cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag
     3181 caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg
     3241 tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg
     3301 agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg
     3361 ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt gaggccagag
     3421 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     3481 tgggaaaagc gcctccccta cccggtagaa ttcactcctc aggtgcaggc tgcctatcag
     3541 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     3601 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     3661 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     3721 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     3781 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     3841 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     3901 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     3961 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     4021 cttctcttat ggagatccct cgacctgcag cccaagcttt agggatcaag atcgccaaaa
     4081 aagaagagaa aggtgccgaa gaagcatgca gcaccaccaa aaaaaaaacg aaaagtagaa
     4141 gacccacgag gcaacaccag cggcgtgctg agcaccccca aggccaagag ggccaagcac
     4201 ccccccggca ccgagaagcc caggagcagg agccagagcg agcagcccgc cacctgcccc
     4261 atctgctacg ccgtgatcag gcagagcagg aacctgagga ggcacctgga gctgaggcac
     4321 ttcgccaagc ccggcgtgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     4381 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     4441 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     4501 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     4561 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4621 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4681 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4741 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4801 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaggtgaac ttcaataaaa
     4861 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4921 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4981 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5041 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5101 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5161 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5221 ttttggaggc ctagagcttg tttttatttg tatctttaaa ttttatgata gatgttatag
     5281 tgtactctcc tctcccctcc cctcccctct cctcctcagc aggatcttgt tatgtagccc
     5341 taagggatct aatacttact ctgtagtcca ggctgtcctt cagctcatga gacacaccat
     5401 catgcttggc atgtttcttg ttctgaaagg tatctggttt taattttggt tgagctggtg
     5461 tgttggtgta tatctgtaat ctcagcacag gcaagtagag acaaggatta tgattacagt
     5521 ccagcctggg ctatttagag ggattctctt taatctttcc tcatgcccca aaatcttacc
     5581 tttggtatat gaaaaatagt ctccacttct gcaaaatatt gctttatgaa gtaagaattc
     5641 ccttcataga gacaaggaat gtgtcctgta aaagtttaat gtgtaagaag tatttgttat
     5701 aaaagataaa tattcagaat cttcttttta attcctgatt ttatttctat aggactgaaa
     5761 gacttgctcg agatgtcatg aaggagatgg gaggccatca cattgtggcc ctctgtgtgc
     5821 tcaagggggg ctataagttc tttgctgacc tgctggatta cattaaagca ctgaatagaa
     5881 atagtgatag atccattcct atgactgtag attttatcag actgaagagc tactgtgtaa
     5941 gtataattaa cttataatta aaaaaatagg gccattctag ttttatctat atttttttta
     6001 aacttgtgca aactatgcta cataatcaac ttacttgatt atgtaatata tatagtaata
     6061 gttggaatta ctattattac atatttattt ttctgtgctg gggtagaacc cagggaacta
     6121 agtactgtaa taaccgagct tcattcatcc tagtcatttt taattttttt tttttttttt
     6181 gttttttcga gacaaggttt ctctgtgtag ccctggctgt cctggaactc actctgtaga
     6241 ccaggctggc ctcgaactca gaaatctgcc tgcctctgcc tcccagtgct gagattaatg
     6301 acgtgcacca ccactgcctg gcattttttt aaagttttaa tttgagaccc tttctcacta
     6361 acttgctcac actatcctcg aactcattac gtgggctggt ctagaaattt cagtcaactt
     6421 gcctcagctt ctcaagtagc tgggattaca ggcaggtgct accacttctg gccatgatca
     6481 ttgttgttgt tgctattatt attattatta tttcactgat acttcatatc acaacacaga
     6541 atagtaactg tagttcatga ccaacaaaaa agttgtacag tttctctgtt gtcttatttt
     6601 gatctggatt ttgatttgtt tcatggcact gggcaggcta atctcaaact gcatggctca
     6661 aatagtcctt ctgtcttaat ctcccatgtt ctggaagtat aggtatcact acacctagct
     6721 gtattttcat attttaaaaa aaacgcctaa gaattgctat tgaatgaggc ggagagatgg
     6781 ctcagcagtc acaagtactt gtagaggact ggggtttggt tactagcacc tcaaagatgg
     6841 cttacaatta tctgtagctc caattccaga ggatctgtgt ggtaaagctg catgtatatg
     6901 ggcatgcagg taaaactgct aaacatataa aaatagctca gtcttaagag aattggtcga
     6961 cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac aattcactgg
     7021 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     7081 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     7141 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     7201 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7261 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7321 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7381 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7441 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7501 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7561 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     7621 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7681 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7741 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7801 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7861 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7921 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7981 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8041 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8101 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8161 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8221 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8281 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8341 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8401 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8461 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8521 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8581 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8641 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8701 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8761 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8821 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8881 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8941 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9001 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9061 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9121 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9181 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9241 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9301 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9361 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9421 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9481 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9541 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9601 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9661 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     9721 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     9781 ccatgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggagctcca
     9841 ccgcggtggc ggccgctcta gaactagtgg atccgcggcc gctaattcga ttcttctgta
     9901 aaattatctt attagtaagt tgtaaccatc tcagacttct aagtttgagt ttgtaaagga
     9961 tcagtttttt ttggtgacct acacacacac acacacacac acacacacac acacacacag
    10021 gggggtggat tctgagaatt tagagaattt agatggttaa taccagtttt tatttttatt
    10081 ctgtgtgata tttatttatt aagatttatt tatttattac atgtaggtac actgtatctg
    10141 tcttcagaca caccagaaga gggcatcaga tctcgttaca gatggttgtg agtcaccatg
    10201 tggttgctgg gatttgaact caggacctct ggaagaacag tcagtgctct taaccgttga
    10261 gccatctcac cagccccgtg atattttatt taacttacta gaatatactg tgttaaaaca
    10321 tattttaggg gattggggag atggcccatt ctgtaaagtg tctgccatgt catcttcagg
    10381 acctgcgttt gtaaccccag catggagaag acagagacag ggagacccct gggcttcctg
    10441 gcaagctaga ctagcaaatt tggttaacta ggttcaatga gagactcagt ctcaaaacat
    10501 aagaagtgaa ttttttagac ccaatttcaa tctctgaact ccatacgtat gcacatgtac
    10561 ctgcatataa atatatatac acacccataa acacatgcac acacaaaaaa tggttcaaaa
    10621 gttcttaaaa tgtataagag tagaaagtgt agagccggga gtggtggcgc acgcctttaa
    10681 tcccagcact tgggagcaga ggcaggtgga tttctgagtt cgaggccagc ctggtctaca
    10741 gagtgagttc caggacagcc agagctatac agagaaatcc tgcctcaaaa aaccaaaaat
    10801 aaagtgtata tactaatctt tcatgcctaa aatatttatt tttgccacat ttttcatacc
    10861 tctttaatat ttttcttttt ctctttgtgc ttcaaaggaa attttgtgtc atgttttact
    10921 tttcattctt gactctattc agcagtaaga cgcagcatgg ctttcttaga gaagtataat
    10981 ctattacaaa acggtaacat ttaactgctc tacataccct cttgtagcta aattcctctg
    11041 ttaaactaag acttattatc taaacgtaat taaatgttga tattattgaa gcatttatca
    11101 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    11161 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt
    11221 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    11281 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    11341 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    11401 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
    11461 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    11521 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
    11581 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
    11641 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
//
