LOCUS       sequence-229792-        2272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Paleologou KE, Schmid AW, Rospigliosi CC, Kim HY, Lamberto GR, 
            Fredenburg RA, Lansbury PT Jr, Fernandez CO, Eliezer D, Zweckstetter
            M, Lashuel HA
  TITLE     Phosphorylation at Ser-129 but not the phosphomimics S129E/D 
            inhibits the fibrillation of alpha-synuclein.
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16895-905. Epub 2008 Mar 14.
  PUBMED    18343814
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16895-905. 
            Epub 2008 Mar 14"
COMMENT     Sequence Label: pT7-7 asyn WT
FEATURES             Location/Qualifiers
     source          1..2272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     111..133
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     198..217
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        198..216
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             248..270
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(829..1620,1621..1689))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1452..1471
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1690..1794)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1862..1880
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
       61 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      121 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcccagcg attcgaactt
      181 ctgatagact tcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag
      241 aaataatttt gtttaacttt aagaaggaga tatacatatg gatgtattca tgaaaggact
      301 ttcaaaggcc aaggagggag ttgtggctgc tgctgagaaa accaaacagg gtgtggcaga
      361 agcagcagga aagacaaaag agggtgttct ctatgtaggc tccaaaacca aggagggagt
      421 ggtgcatggt gtggcaacag tggctgagaa gaccaaagag caagtgacaa atgttggagg
      481 agcagtggtg acgggtgtga cagcagtagc ccagaagaca gtggagggag cagggagcat
      541 tgcagcagcc actggctttg tcaaaaagga ccagttgggc aagaatgaag aaggagcccc
      601 acaggaagga attctggaag atatgcctgt ggatcctgac aatgaggctt atgaaatgcc
      661 ttctgaggaa gggtatcaag actacgaacc tgaagcctaa gaaatatctt tgctcccagt
      721 ttcttgagat ctgctgacag atgttccatc caagcttatc gatgataagc tgtcaaacat
      781 gagaattaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      841 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      901 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      961 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1021 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1081 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1141 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1201 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1261 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1321 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1381 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1441 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1501 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1561 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1621 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1681 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1741 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1801 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     1861 aaataggcgt atcacgaggc cctttcgtct tcaagaataa aaggatctag gtgaagatcc
     1921 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1981 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2041 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2101 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2161 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2221 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt ct
//
