LOCUS       sequence-229817-        8157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene constitutive expression from a Pc promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Xu Y, Tao F, Ma C, Xu P
  TITLE     New constitutive vectors: useful genetic engineering tools for 
            biocatalysis.
  JOURNAL   Appl Environ Microbiol. 2013 Apr;79(8):2836-40. doi: 
            10.1128/AEM.03746-12. Epub 2013 Feb 15.
  PUBMED    23416993
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03746-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-04"; volume: "79"; issue: "8"; pages: "2836-40"
COMMENT     Sequence Label: pMMPc-Gm
FEATURES             Location/Qualifiers
     source          1..8157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(530..535,536..552,553..558)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             747..1280
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     CDS             complement(2551..3402)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3389..4228)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(4739..5710)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            6949..7036
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      7378..7772
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 tcgctttcag aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
       61 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
      121 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
      181 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
      241 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
      301 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
      361 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
      421 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
      481 cttcggtcct ccgatcgctg attccctttg tcaacagcaa tggatcgaat tgacataagc
      541 ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
      601 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      661 tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
      721 atgtttgatg ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg
      781 gcagtcgccc taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc
      841 tcggccctga ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg
      901 gagacgtagc cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc
      961 gtagtaagac attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg
     1021 cggcttacgt tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg
     1081 cagtctccgg cgagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc
     1141 atgaggccaa cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc
     1201 ccgcagtggc tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg
     1261 acccaagtac cgccacctaa aagcttggct gcaggtcgac ggatccccgg gaattcacgg
     1321 gttcgctacc tgcattacag cctacagacc gagaaggctt atgtctactg ggccaaggca
     1381 tttgtgttgt ggacggcccg cagccatggt gggtttcgac atccgcgcga aatggggcaa
     1441 gctgaagtcg agggttttct gaccatgctc gccaccgaga agcaagtggc gccggccacc
     1501 caccggcagg cgctcaacgc gctgttgttc ttgtatcggc aacgcgtcag tgggctgatc
     1561 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     1621 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     1681 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     1741 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     1801 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     1861 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     1921 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     1981 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
     2041 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     2101 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     2161 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2221 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2281 cgcaattaat gtaagttagc gcgaattgca agctggccga cgcgctgggc tacgtcttgc
     2341 tggcgttcgg gagcagaaga gcatacatct ggaagcaaag ccaggaaagc ggcctatgga
     2401 gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt ttctgagcat
     2461 ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat aaaagataaa
     2521 aatgtcttgt ttacaataga gtgggggggg tcagcctgcc gccttgggcc gggtgatgtc
     2581 gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct ccggcaacgc
     2641 ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg acggccagac
     2701 atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga tccagccaca
     2761 cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct cgtccatgct
     2821 gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt tcagggccac
     2881 gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa acccctgccg
     2941 cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc agtcctgtat
     3001 gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca gcgcccgata
     3061 gctacctttg accaccatgg catcagcggt gacggcctcc cacttgggtt ccaggaacag
     3121 ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc cattaggccc
     3181 agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg gctccgggcc
     3241 gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct tgctgcgctt
     3301 gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg ccgggtcgtg
     3361 ccggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc cccttgctct
     3421 tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc ctgaaccacc
     3481 gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg aagaaccggc
     3541 gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc gacaggtagg
     3601 actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc tgctggtcgc
     3661 ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata gagcacccgg
     3721 tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg ctggcgtttt
     3781 cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg ccctcggcgg
     3841 cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg ccgatcagcg
     3901 gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg tgcgccccaa
     3961 gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg tagatcaccg
     4021 ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc tgtgcggcca
     4081 gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc ccgaccgtac
     4141 cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg aacgcctcca
     4201 gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag ttggtggtta
     4261 ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca ggcactcgcg
     4321 cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc ctttggcctt
     4381 catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca ggtcgccggt
     4441 ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg ccgaaaggct
     4501 tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc atatcagcga
     4561 ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg ggcaaccaat
     4621 agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg tattccataa
     4681 aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa cgcaagcact
     4741 acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc ggtgcccgtg
     4801 ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg acggtgcgct
     4861 cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg gcctcggcca
     4921 tggccttgcc gatttcctcg gcactgcggc cccggctggc cagcttctgc gcggcgataa
     4981 agtcgcactt gctgaggtca tcaccgaagc gcttgaccag cccggccatc tcgctgcggt
     5041 actcgtccag cgccgtgcgc cggtggcggc taagctgccg ctcgggcagt tcgaggctgg
     5101 ccagcctgcg ggccttctcc tgctgccgct gggcctgctc gatctgctgg ccagcctgct
     5161 gcaccagcgc cgggccagcg gtggcggtct tgcccttgga ttcacgcagc agcacccacg
     5221 gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc gccaagcggc
     5281 catagtggcg gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc agcgtccggg
     5341 caatctgccc ccgaagttca ccgcctgcgg cgtcggccac cttgacccat gcctgatagt
     5401 tcttcgggct ggtttccact accagggcag gctcccggcc ctcggctttc atgtcatcca
     5461 ggtcaaactc gctgaggtcg tccaccagca ccagaccatg ccgctcctgc tcggcgggcc
     5521 tgatatacac gtcattgccc tgggcattca tccgcttgag ccatggcgtg ttctggagca
     5581 cttcggcggc tgaccattcc cggttcatca tctggccggt ggtggcgtcc ctgacgccga
     5641 tatcgaagcg ctcacagccc atggccttga gctgtcggcc tatggcctgc aaagtcctgt
     5701 cgttcttcat cgggccacca agcgcagcca gatcgagccg tcctcggttg tcagtggcgt
     5761 caggtcgagc aagagcaacg atgcgatcag cagcaccacc gtaggcatca tggaagccag
     5821 catcacggtt agccatagct tccagtgcca cccccgcgac gcgctccggg cgctctgcgc
     5881 ggcgctgctc acctcggcgg ctacctcccg caactctttg gccagctcca cccatgccgc
     5941 ccctgtctgg cgctgggctt tcagccactc cgccgcctgc gcctcgctgg cctgctgggt
     6001 ctggctcatg acctgccggg cttcgtcggc cagtgtcgcc atgctctggg ccagcggttc
     6061 gatctgctcc gctaactcgt tgatgcctct ggatttcttc actctgtcga ttgcgttcat
     6121 ggtctattgc ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg gtgtcgatgt
     6181 tcagggccac gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc accacgttcg
     6241 gccccaggtg aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt ctgtggtcaa
     6301 tgcgggcgtc gtggccagcc cgctctaatg cccggttggc atggtcggcc catgcctcgc
     6361 gggtctgctc aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc ccgcccttct
     6421 ccggggtctt gccgttgtac cgcttgaacc actgagcggc gggccgctcg atgccgtcat
     6481 tgatccgctc ggagatcatc aggtggcagt gcgggttctc gccgccaccg gcatggatgg
     6541 ccagcgtata cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg gacgccagcg
     6601 ccttctgctg gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc ttgaacagcc
     6661 gcccattggc gcgttcatac aggtcggcag catcccagta gtcggcgggc cgctcgacga
     6721 actccggcat gtgcccggat tcggcgtgca agacttcatc catgtcgcgg gcatacttgc
     6781 cttcgcgctg gatgtagtcg gccttggccc tggccgattg gccgcccgac ctgctgccgg
     6841 ttttcgccgt aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc ttttcggctc
     6901 catgcaatgg ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc agtttctcga
     6961 agagaaaccg gtaagtgcgc cctcccctac aaagtagggt cgggattgcc gccgctgtgc
     7021 ctccatgata gcctacgaga cagcacatta acaatggggt gtcaagatgg ttaaggggag
     7081 caacaaggcg gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa tcaatgccga
     7141 aattcagcgg gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa caaggcgcaa
     7201 ggtgctggtg ggggccatga ttttggccaa ggtgaacagc agcgagtggc cggaggatcg
     7261 gctcatggcg gcaatggatg cgtaccttga acgcgaccac gaccgcgcct tgttcggtct
     7321 gccgccacgc cagaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc
     7381 tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct
     7441 ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct
     7501 gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct
     7561 ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct
     7621 agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc
     7681 ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg
     7741 caggggggcg tgacagttat tgcaggggtt cgtgacagtt agtacgggag tgacgggcac
     7801 tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta
     7861 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
     7921 ccaggacggc cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa
     7981 cccttctcta tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc
     8041 agggaaagga attgccgggc tatgtgcaac gggaatttga agaatttctc caatgcgggc
     8101 ggctggagca tggctttcta cgggttcgct gcgagtcttg ccacgccgag cacctgg
//
