LOCUS       sequence-229821-       10879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Pavicic M, Mouhu K, Wang F, Bilicka M, Chovancek E, Himanen K
  TITLE     Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin 
            E3 Ligases in Arabidopsis.
  JOURNAL   Front Plant Sci. 2017 Mar 28;8:416. doi: 10.3389/fpls.2017.00416. 
            eCollection 2017.
  PUBMED    28400782
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-03-28"; volume: "8:416. doi"; pages: " 
            10.3389/fpls.2017.00416. eCollection 2017"
COMMENT     Sequence Label: pBA cHA-XERICO
FEATURES             Location/Qualifiers
     source          1..10879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(835..1626)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2147..2171
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     2371..2393
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2385..2402
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2386..2402
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2462..3103)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(3147..3698)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(3749..3928)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4838..5183
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5126..5146
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    5216..5240
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5730..5754)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5765..5791
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5771..5797
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5801..5827
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5807..5833
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5837..5863
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5843..5869
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     terminator      5923..6175
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6192..6208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6192..6208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6205..6227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6216..6232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6240..6246,6247..6264,6265..6270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6285..6306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6464..6488
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7819..8448
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8877..9950
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10016..10210
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10446..10468)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10554..10694
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10609..10628
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10709..10726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgcatgat atatctccca atttgtgtag ggcttattat gcacgcttaa aaataataaa
      721 agcagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca tctaacgctt
      781 gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta gacattattt
      841 gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc
      901 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac
      961 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc
     1021 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc
     1081 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa
     1141 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac
     1201 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc
     1261 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc
     1321 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag
     1381 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg
     1441 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt
     1501 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg
     1561 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc
     1621 ccccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
     1681 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     1741 agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
     1801 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta
     1861 cagcttacga accgaacagg cttatgtcca ctgggttcgt gcccgaattg atcacaggca
     1921 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     1981 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     2041 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     2101 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     2161 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
     2221 actgaattaa cgccgaattg ctctagcatt cgccattcag gctgcgcaac tgttgggaag
     2281 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     2341 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2401 gtgccaagct tgcatgcctg caggtcgact ctagtgggta ccagcttggg ctgcaggtcg
     2461 attgatgcat gttgtcaatc aattggcaag tcataaaatg cattaaaaaa tattttcata
     2521 ctcaactaca aatccatgag tataactata attataaagc aatgattaga atctgacaag
     2581 gattctggaa aattacataa aggaaagttc ataaatgtct aaaacacaag aggacatact
     2641 tgtattcagt aacatttgca gcttttctag gtctgaaaat atatttgttg cctagtgaat
     2701 aagcataatg gtacaactac aagtgtttta ctcctcatat taacttcggt cattagaggc
     2761 cacgatttga cacattttta ctcaaaacaa aatgtttgca tatctcttat aatttcaaat
     2821 tcaacacaca acaaataaga gaaaaaacaa ataatattaa tttgagaatg aacaaaagga
     2881 ccatatcatt cattaactct tctccatcca tttccatttc acagttcgat agcgaaaacc
     2941 gaataaaaaa cacagtaaat tacaagcaca acaaatggta caagaaaaac agttttccca
     3001 atgccataat actcaaactc agtaggattc tggtgtgtgc gcaatgaaac tgatgcattg
     3061 aacttgacga acgttgtcga aaccgatgat acgaacgaaa gctctagcta gaggatcagg
     3121 cctgaattca gatcctctag gggtcatcag atctcggtga cgggcaggac cggacggggc
     3181 ggtaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
     3241 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
     3301 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
     3361 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag
     3421 acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg
     3481 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
     3541 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
     3601 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
     3661 gcacggcgga tgtcggccgg gcgtcgttct gggctcatgg tagatccccc tagagtcgag
     3721 atctggattg agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt
     3781 cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg
     3841 aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg
     3901 ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg
     3961 cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat cttgatcccc
     4021 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
     4081 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
     4141 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
     4201 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
     4261 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc
     4321 agcgtgaagc ttgcatgcct gcaggtcccc agattagcct tttcaatttc agaaagaatg
     4381 ctaacccaca gatggttaga gaggcttacg cagcaggtct catcaagacg atctacccga
     4441 gcaataatct ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     4501 tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta
     4561 ttccagtatg gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag
     4621 tctctaaaaa ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg
     4681 acctaacaga actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca
     4741 atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa
     4801 atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa
     4861 tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag
     4921 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg
     4981 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     5041 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     5101 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     5161 gttcatttca tttggagaga acacggggga ctctagagga tctcgaggcg cgcccacaag
     5221 tttgtacaaa aaagcaggct ctatgggtct atcaagtctt cctggtccat cagaaggaat
     5281 gttatgtgtg atattagtta atacagcatt atcgatctcc attgtcaaag gcattgtaag
     5341 atcattcctt ggcatagtag gaatcagtct ctcgccgtct tcatcctcgc cttcttcggt
     5401 gacggtatct tcagagaatt catcaacttc agagtcattt gatttccggg tctgccaacc
     5461 agagagttac cttgaggagt tcaggaaccg gactccgaca ctgaggtttg agagcttgtg
     5521 caggtgcaag aaacaggcag acaatgagtg ttctgtgtgt ttgtcgaaat tccaagggga
     5581 ttcagagatc aacaagctca agtgcggcca tttgtttcac aaaacatgct tggagaaatg
     5641 gatagactat tggaacatca cttgcccatt gtgtaggact cctcttgttg ttgtgccaga
     5701 agaccatcag ctttcttcta atgtttggaa cccagctttc ttgtacaaag tggttacgta
     5761 catgtatcct tatgatgttc cagattatgc tagtcttatg tacccatacg acgtgcctga
     5821 ctacgcatca ttgatgtatc cttatgatgt tccagattat gctagtcttc ccgggcctag
     5881 gtgtacagac gtcttaatta actagtgagc tcgaatttcc ccgatcgttc aaacatttgg
     5941 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     6001 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     6061 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     6121 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     6181 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6241 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6301 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattggagc ttgagcttgg
     6421 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
     6481 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     6541 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     6601 aaagtacttt aaagtacttt aaagtacttt aaagtacttt gatccaaccc ctccgctgct
     6661 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     6721 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     6781 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     6841 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     6901 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     6961 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     7021 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     7081 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     7141 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     7201 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     7261 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     7321 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     7381 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     7441 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     7501 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     7561 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     7621 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     7681 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     7741 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     7801 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     7861 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     7921 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     7981 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     8041 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     8101 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     8161 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     8221 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     8281 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     8341 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     8401 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     8461 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     8521 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     8581 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     8641 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     8701 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     8761 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     8821 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     8881 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     8941 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     9001 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     9061 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     9121 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     9181 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     9241 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     9301 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     9361 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     9421 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     9481 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     9541 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     9601 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     9661 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     9721 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     9781 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     9841 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     9901 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     9961 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
    10021 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
    10081 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
    10141 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
    10201 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
    10261 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
    10321 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
    10381 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
    10441 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    10501 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
    10561 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
    10621 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10681 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10741 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10801 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10861 aggccgcgtt gctggcgtt
//
