LOCUS       sequence-229827-       11016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Mallory AC, Bartel DP, Bartel B
  TITLE     MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 
            is essential for proper development and modulates expression of 
            early auxin response genes.
  JOURNAL   Plant Cell. 2005 May . 17(5):1360-75.
  PUBMED    15829600
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2005 May . 17(5):1360-75."
COMMENT     Sequence Label: ARF17 pGreenII0229
FEATURES             Location/Qualifiers
     source          1..11016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    625..647
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(671..923)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(943..1494)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1536..1715)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1947..1969
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1961..1978
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1962..1978
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1988..2007
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1988..2006
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2031..2047
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2032..2048
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8707..8727)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8707..8725)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8746..8762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8746..8762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8759..8781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8770..8786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8794..8800,8801..8818,8819..8824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8839..8860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8977..8994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9099..9123
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9214..9802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9294..9313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9973..10788)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9973..10788)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10696..10715
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10780..10799
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
      541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
      601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcagcttg
      661 catgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
      721 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
      781 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
      841 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
      901 tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcagattt cggtgacggg
      961 caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg
     1021 ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc
     1081 ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa
     1141 gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg
     1201 ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc
     1261 cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca
     1321 gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc
     1381 ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg
     1441 catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcatggtaga
     1501 tcccctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1561 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1621 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1681 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1741 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
     1801 taacattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     1861 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     1921 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     1981 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
     2041 cggtatcgat aagcttgata tcgaattctt aaatcttttt gctgtgttct tataaagaaa
     2101 catcatctat taaagttgtc ttcgtttgga tttggttttg acgactttgg gaaatattta
     2161 tgtttaagaa ggtttcattg gtcattgact tttatatatt atatcgtaac catgatgtga
     2221 tagtgggcct tagatcaaca aacatgcgaa aaacagaagc agaggcccgt ttcaacggag
     2281 cataataaat tgcattctct gtcttttgtt tttaggtttt ttttttaact gatagatgtg
     2341 ccgtcgaaaa taatattgat atttaaaaat tcacaacaaa cattcttaac tgacccaccc
     2401 atctatctgc tattcccacg cgccaaggaa aataataata atagcgaaat tgatttttac
     2461 atttatttat tgatagataa tttgtgtatt gttaagatta acagatttta agggattaaa
     2521 gtggaaaagg taaaccgaag acaacttgcc atttactgat ttacaacaat ccaaatttaa
     2581 aaacaaatgg tcccagtttt tagggttgtc acttaaattt atcgaaatat ttacacttta
     2641 attgggtaaa acataatgga cagaaaaaca aatattgtga caaacaaaaa aacatgtttt
     2701 caccaagaaa aacaaaaaca aaaaagatgt aaagcttttc ttacatctgt acaaaataaa
     2761 agcagacgaa attgtacttt attttcctta ttaaattgtc ggtatgtttt atatgttgtg
     2821 aaaagtagaa tggataacca aataaaaatt actgacatct taataaagtt ggttcaaccg
     2881 gtttaaaatg tattttttta gtgttaacaa cttaaagctt ttttcgatta tcgaattgca
     2941 acaaacaaat atattaacag aaaaaaggaa tcatgtatct atttcaatat cctgtttttt
     3001 ttcttccatt tggatattta gatctttttc tgaatttatc ttgttcttaa attaaaacag
     3061 aaaaaaagat taaaagtaag acagcttgct aatggcaacc gcaacaaaca agataatttt
     3121 gaaacggatc cacttggatt ttctttgatt ttgtagaaaa attgacaaat tgcttttgta
     3181 taaaaacaaa aaatgtaccg taaaaacaca cacataaaaa ataaaaagtg ataatgacaa
     3241 acaaataaag tggtattttt cttttatcta ctaatgtgat tataaaaaaa tcgacattga
     3301 aaatttcaac acatcttttt cgccaaaacc tgaaaatggt cttattataa cataaattag
     3361 tttttttgtc tttctattat atattcaata actcatccca acttgaacaa acctatttca
     3421 aatttttttg tcttgaataa tgataagttc cgtagtgttc ttttctgttg tgacaaaaaa
     3481 tactagctaa cgagggataa gcacaaaaac atgattaatg tttctctaat cattctaaaa
     3541 atctacagga atattccctt ttcagttttt tctttcttaa atgcatttct tagttcttca
     3601 taattcagtg agttttaata acaataataa aaaaaagagc atcattaatt gaacctaaaa
     3661 ataatgagaa gaaaaaccaa aaagatagag agtaagatgc acgcgctaaa gatcgaacgg
     3721 ttaatagaat caggttagtg aagagagata ttaaaagttt gttgtcgtgt ggcaaaaact
     3781 ataatttcct tctcacaaac aaaaaaaata aaatcaaaca caaaatcccg tagcatcgta
     3841 acagtaattc gctattatct cctcaccctc cgctttcgct tcccttctct gcccgtttca
     3901 attccttcta agacatcacg gtctctctct ataaaaacag tacctacctc ttcttcttct
     3961 tcttcattcg ctgacttcgt ttacactgaa aacaaatacc tatgtcaccg ccgtcggcaa
     4021 ccgccggtga catcaaccac cgtgaagtag acccgacgat ctggcgcgct tgtgctggag
     4081 cctccgtcca gatccctgtc cttcactcta gggtttacta ctttccacaa ggtcacgttg
     4141 agcactgttg ccctctcctc tctactcttc cttcctccac ctcgccggtt ccatgtatca
     4201 tcacttcaat tcagttgctc gccgatccgg tcaccgacga ggtctttgct caccttattc
     4261 ttcaaccgat gacgcagcag cagtttactc cgactaatta ttcacgattc ggcagattcg
     4321 atggcgatgt tgatgataac aacaaggtga ctaccttcgc caaaattctc acgccttctg
     4381 atgctaacaa tggaggtggc ttctccgttc ctcgtttctg tgctgattcc gtcttccctc
     4441 tgcttaattt tcaaatcgat ccaccggttc agaagctcta cgtcactgat atccatggag
     4501 ctgtttggga tttcaggcat atctatcgcg gtacaccgag gcgtcacttg ctaacaacgg
     4561 gatggagtaa gttcgtcaat agcaagaagc tcatcgctgg agattcggtg gtgtttatga
     4621 gaaaatctgc agatgagatg ttcatcggcg ttaggagaac tccgatctca agcagcgacg
     4681 gaggaagtag ctattacgga ggagatgagt ataacggtta ctacagtcag agtagcgttg
     4741 ccaaggaaga tgatgggagt ccgaagaaga cgtttaggag atctgggaat ggtaagttga
     4801 ctgctgaggc tgtgacggat gcgatcaata gagcgtctca gggattacca tttgaggtgg
     4861 tgttttatcc ggctgctgga tggtctgagt ttgttgtgag agctgaagat gttgagtctt
     4921 caatgtctat gtattggact cctgggactc gagtcaagat ggctatggag actgaagatt
     4981 cttctcggat cacatggttt caaggcatcg tttcctctac ttatcaggag accggtccat
     5041 ggcgtggatc tccatggaag cagcttcagg tatatgatgt ttttgaaatg gtctttgctc
     5101 ttcttatctc tataatgttg cgttaatgga acaattcaga atcgatcttg tatctgttgt
     5161 gtgcaagcct ttcatgagta gtttaagtct catcctggtt attcaaatgt caattgggtt
     5221 ttgaatgttg ttttgattgc tgtgttgttg ttttgaaggt aaatattgga aacaggataa
     5281 gttaattcat acgaaaatga atgttctctc tcagattcat cttctataag atggaattga
     5341 aactggaaga tttggcttag tattgtttgt gttgagcgtc cgtgatgtag agttgctttt
     5401 cattatcctt ctttggccac gcattgtaca ttgtgtttgt taaactagag ttcctctgat
     5461 tagtcttatg agatactcct tttttgccaa tatattctac ttcctctgat tagttccttt
     5521 gtttttaact tgcgtagatc acatgggatg aacctgagat tctgcaaaac gtgaagaggg
     5581 tgaatccatg gcaagtggaa attgctgcac atgcaactca actgcatacc cctttccctc
     5641 cagcaaagag gttgaagtat ccacaacccg gaggagggtt cttgagtgga gatgatggag
     5701 aaatccttta tcctcaaagt ggactgtcta gtgcagcagc acctgatcca agtccttcta
     5761 tgttctcgta ttctacattt cctgctggca tgcagggagc caggcaatat gattttgggt
     5821 ctttcaatcc aaccggattc attggaggaa atcctcccca gctattcacc aataacttct
     5881 taagtccgct tcctgatttg ggaaaagtgt cgactgagat gatgaacttt ggcagtccgc
     5941 catcagataa cttatcgcct aatagcaaca ccactaatct gtcctctgga aatgacctgg
     6001 ttggaaaccg aggccccctt tcaaagaaag ttaactcgat tcagttgttt ggcaagatca
     6061 ttaccgtgga ggagcattct gagagcggtc ctgcagagtc tggcttgtgt gaagaggatg
     6121 gcagcaaaga gtccagcgac aatgagacac agttgtcctt atcacatgct cctccaagcg
     6181 tgcctaaaca ttccaacagc aacgcaggtt ctagctccca aggtatattc cgatctctct
     6241 caagtacaat aatcaattga atcagttgct ataagctttt attactgttt tgcacaaggc
     6301 aatttctctt cctttcccat gaactatatt atgtagagta ggaaacacaa tcatgatttc
     6361 tgatatgact tgactgatga tgatacttgt gaaaactatc tatatatctc ttcagtaatc
     6421 agtcgccttg aggtaattgg aatttggaac ttgaacatta cttggatttt aacttttcaa
     6481 tagcataagc tttcctgttt catcatatat gtttcactat acttggtatg cttttattac
     6541 tgctgatatt tactattcct gctatttttt ttgggtctcg ttaacggtaa taaggacaca
     6601 gaattggctc ttttatccat ctgaactaga cattactgta caagtagatg aagaattatg
     6661 tggttccatt acaaatttga tttgcagaaa gcttgaagct gctgcttata gacgattata
     6721 atgttggaag atcctgaagc ttggaatgat ttgtactttt cttctgtttg tgtgtgtttt
     6781 gacaggttaa aaagtgaaag aagtggtgga tctttgctgg aatctccaag tcctaagtag
     6841 tagtagtagt actattatat ataattctgt tgtttctgca attgactttt ctctggcttt
     6901 tctttgccac gtgacgattc cgttttttac tttctttctt ttttttttat caatttctca
     6961 gacacatttg atgaacatct cgctctcatc taatcgttaa ctatttttat tggggtaaat
     7021 gtctggattt gtcttaccta aacatgtttt aagactgatg tttatgcaga gtgaaaacag
     7081 caaataattt aatgctttat tcaatcccta tgcaatggat ctaacttaac ggcgccaacc
     7141 agagagtttt actaactgtt ttttgctttt agttaatatt cctaataaat aaaaagactg
     7201 ccaataataa aatcggacca tttttattct cctaataaat aaaagaagct caagggaggt
     7261 ccctcctaca cttttctgac tcctttatgt tctgttctct gtgattcatt aacggatcag
     7321 ctatagcatt tccaatttgt cagtaagtta gggttggttt gattagctaa tagctaccaa
     7381 tgagacaaaa tatattaggt tggtttgatt agcaattaag acgacgactt gaattatgca
     7441 taaaaagaca tttgagattt gaagatctca ttaaacaatt ggaggagact tggctccatg
     7501 tctctcactg ttttgcacgg taataggagt actacattat ataaatttgg taaaactaga
     7561 tcaacatcag attgtttcaa tgtttatgtg tacttgggaa gagtggccct gcaggtctta
     7621 atttaagttt cctcaagtgg taatagtatg aaaaaatata aacgtatatg ttcttggaca
     7681 attagctgta agttacagcc tgtaacgcaa acttgagagt gtaaataagg acaacaacaa
     7741 caaaagtaaa taagaaaatt tcagaaatat ggttatgtgt tgaggagaca caaatcattc
     7801 ctggttctga cttcaatcat actctctgca taaaagaagt agtagtagta gtacataagg
     7861 acaaaaaact attaaatgta attatttgat ctacaaaatg ttatttcaac ttgagcaaaa
     7921 aaatgtaaaa tgttaatttc aaaataaaaa taaaaagaag caagtgggtg aagtttctgc
     7981 atggtaaaaa gtgtgaaaga agaacatgag aagaggagtg gtgtcctctg cggaagaggc
     8041 aaagcctgac tcaagttttc tagtaaaaaa cactttccac atagcatttt tttttttttt
     8101 tttttattat tatcatactt ttggtttcct gttaattgat atttgatgat gctgtcctct
     8161 ctctatctcc cgactcccat atccttataa tttgatgtgg attggacaaa acaaatgaat
     8221 gaaaacgcat caaacagagc aaataatact aaataaacaa gataaatatt ggagagaaag
     8281 aaaaaaacaa gaaaattgct aataatattt tgagaaataa aatcaaacaa aaaaaaaaac
     8341 tcatcataaa aaaagtagag cacgagatgc tttctcactc tctctgtgga tcactctgtc
     8401 ttgcatctaa gtcaaatcaa gtccaatcct caggttttta atcatttgct tctttttttt
     8461 tgtcttgacc aaattcattt tattacctcc cctgcaactc ttttccgtat agaaaagaca
     8521 actgtcacaa gctactctct ttcgcagtca atttttattc aaaaagcaac ccacaacggc
     8581 caaaaaagtg taatagaaga ttcaaatgga atacttttac ttagaaacct ttttaacttt
     8641 cacattttaa cttctacaaa cagtactaga gcggccgcca ccgcggtgga gctccagctt
     8701 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     8761 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8821 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8881 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8941 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9001 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9061 agaatcaggg gataacgcag gaaagaacat gaaggccttg acaggatata ttggcgggta
     9121 aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc
     9181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9661 atttggtatc tgcgctctgc tgaagccagt taccttcgga agaagagttg gtagctcttg
     9721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgtgtaaca
     9961 ttggtctagt gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
    10021 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
    10081 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
    10141 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
    10201 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
    10261 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
    10321 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
    10381 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
    10441 tgaatcagga tattcttcta atacctggaa tgctgttttc cctgggatcg cagtggtgag
    10501 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
    10561 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
    10621 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc
    10681 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
    10741 atttaatcgc ggccttgagc aagacgtttc ccgttgaata tggctcataa caccccttgt
    10801 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
    10861 aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc
    10921 tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca
    10981 aaagttcaaa atcaccaact ggtccaccta caacaa
//
