LOCUS       sequence-229829-       11467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11467)
  AUTHORS   Pavicic M, Mouhu K, Wang F, Bilicka M, Chovancek E, Himanen K
  TITLE     Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin 
            E3 Ligases in Arabidopsis.
  JOURNAL   Front Plant Sci. 2017 Mar 28;8:416. doi: 10.3389/fpls.2017.00416. 
            eCollection 2017.
  PUBMED    28400782
REFERENCE   2  (bases 1 to 11467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-03-28"; volume: "8:416. doi"; pages: " 
            10.3389/fpls.2017.00416. eCollection 2017"
COMMENT     Sequence Label: pBA cMYC-PHL2
FEATURES             Location/Qualifiers
     source          1..11467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(835..1626)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2147..2171
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     2371..2393
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2385..2402
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2386..2402
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2462..3103)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(3147..3698)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(3749..3928)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4838..5183
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5126..5146
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    5216..5240
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6162..6186)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6218..6247
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6257..6286
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6296..6325
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6335..6364
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6398..6427
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      6511..6763
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6780..6796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6780..6796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6793..6815)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6804..6820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6828..6834,6835..6852,6853..6858))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6873..6894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7052..7076
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8407..9036
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9465..10538
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10604..10798
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11034..11056)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11142..11282
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11197..11216
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11297..11314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgcatgat atatctccca atttgtgtag ggcttattat gcacgcttaa aaataataaa
      721 agcagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca tctaacgctt
      781 gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta gacattattt
      841 gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc
      901 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac
      961 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc
     1021 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc
     1081 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa
     1141 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac
     1201 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc
     1261 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc
     1321 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag
     1381 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg
     1441 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt
     1501 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg
     1561 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc
     1621 ccccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
     1681 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     1741 agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
     1801 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta
     1861 cagcttacga accgaacagg cttatgtcca ctgggttcgt gcccgaattg atcacaggca
     1921 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     1981 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     2041 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     2101 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     2161 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
     2221 actgaattaa cgccgaattg ctctagcatt cgccattcag gctgcgcaac tgttgggaag
     2281 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     2341 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2401 gtgccaagct tgcatgcctg caggtcgact ctagtgggta ccagcttggg ctgcaggtcg
     2461 attgatgcat gttgtcaatc aattggcaag tcataaaatg cattaaaaaa tattttcata
     2521 ctcaactaca aatccatgag tataactata attataaagc aatgattaga atctgacaag
     2581 gattctggaa aattacataa aggaaagttc ataaatgtct aaaacacaag aggacatact
     2641 tgtattcagt aacatttgca gcttttctag gtctgaaaat atatttgttg cctagtgaat
     2701 aagcataatg gtacaactac aagtgtttta ctcctcatat taacttcggt cattagaggc
     2761 cacgatttga cacattttta ctcaaaacaa aatgtttgca tatctcttat aatttcaaat
     2821 tcaacacaca acaaataaga gaaaaaacaa ataatattaa tttgagaatg aacaaaagga
     2881 ccatatcatt cattaactct tctccatcca tttccatttc acagttcgat agcgaaaacc
     2941 gaataaaaaa cacagtaaat tacaagcaca acaaatggta caagaaaaac agttttccca
     3001 atgccataat actcaaactc agtaggattc tggtgtgtgc gcaatgaaac tgatgcattg
     3061 aacttgacga acgttgtcga aaccgatgat acgaacgaaa gctctagcta gaggatcagg
     3121 cctgaattca gatcctctag gggtcatcag atctcggtga cgggcaggac cggacggggc
     3181 ggtaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
     3241 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
     3301 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
     3361 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag
     3421 acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg
     3481 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
     3541 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
     3601 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
     3661 gcacggcgga tgtcggccgg gcgtcgttct gggctcatgg tagatccccc tagagtcgag
     3721 atctggattg agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt
     3781 cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg
     3841 aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg
     3901 ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg
     3961 cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat cttgatcccc
     4021 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
     4081 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
     4141 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
     4201 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
     4261 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc
     4321 agcgtgaagc ttgcatgcct gcaggtcccc agattagcct tttcaatttc agaaagaatg
     4381 ctaacccaca gatggttaga gaggcttacg cagcaggtct catcaagacg atctacccga
     4441 gcaataatct ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     4501 tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta
     4561 ttccagtatg gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag
     4621 tctctaaaaa ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg
     4681 acctaacaga actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca
     4741 atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa
     4801 atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa
     4861 tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag
     4921 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg
     4981 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     5041 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     5101 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     5161 gttcatttca tttggagaga acacggggga ctctagagga tctcgaggcg cgcccacaag
     5221 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgtactcag cgattcgctc
     5281 gcttccactc gatggtggac acgttggtgg tgactaccat ggacctcttg acggaaccaa
     5341 tcttcccggt gacgcttgtt tggttttaac gactgaccct aaacctcgtc tccggtggac
     5401 aactgagctt catgagagat tcgttgacgc cgttactcag ctcggtggtc ctgacaaagc
     5461 gactcccaaa actattatga gaacaatggg agtgaagggt ctcactctct accacctcaa
     5521 atcacatctt cagaaattcc gcctagggag gcaagctggc aaagaatcaa ctgagaactc
     5581 taaagatgct tcttgtgtag gggagagtca ggacacaggt tcatcttcga catcatcaat
     5641 gagaatggcg cagcaggagc agaacgaggg ttaccaagtc accgaagctc tacgtgctca
     5701 gatggaagtc caaagaagac tacacgatca attggaggtg caacggaggc tccagctgag
     5761 gatagaggca caaggaaaat acctgcaatc gattcttgaa aaagcttgca aggcctttga
     5821 cgagcaagct gctacttttg ctggacttga ggctgctagg gaagagctat cagagctagc
     5881 catcaaagtc tccaatagct ctcaaggaac atcagtcccg tacttcgatg caacaaagat
     5941 gatgatgatg ccatcgttgt cagagcttgc agtagcaata gacaacaaaa acaacatcac
     6001 aaccaactgt tcagtagaaa gctctctgac ttccatcaca catgggagct ctatatctgc
     6061 tgcatcaatg aagaagcgtc aacgtggaga caatttgggc gtagggtatg aatcaggctg
     6121 gattatgcct agtagcacca ttggaaaggg tgggcgcgcc gacccagctt tcttgtacaa
     6181 agtggtgacg tctcgacggt atcgattcaa agctatggag caaaagctca tttctgaaga
     6241 ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg aaatggagca
     6301 aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt ctgaagagga
     6361 cttgaatgaa atggagagct tgggcgacct caccatggag caaaagctca tttctgaaga
     6421 ggacttgaat tcggtacccc gggttcgaaa tcgatggatc ctacgtagcc taggacgcgt
     6481 cttaattaac tagtgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     6541 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     6601 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     6661 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     6721 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cgtaatcatg
     6781 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6841 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6901 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6961 ggccaacgcg cggggagagg cggtttgcgt attggagctt gagcttggat cagattgtcg
     7021 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     7081 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     7141 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttaa
     7201 agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     7261 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     7321 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     7381 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     7441 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     7501 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     7561 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     7621 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     7681 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     7741 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     7801 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     7861 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     7921 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     7981 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     8041 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     8101 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     8161 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
     8221 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     8281 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     8341 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     8401 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     8461 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     8521 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     8581 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     8641 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     8701 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     8761 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     8821 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     8881 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     8941 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     9001 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     9061 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     9121 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     9181 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     9241 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     9301 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     9361 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
     9421 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     9481 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     9541 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     9601 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     9661 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     9721 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     9781 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     9841 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     9901 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     9961 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
    10021 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
    10081 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
    10141 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
    10201 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
    10261 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
    10321 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    10381 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    10441 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    10501 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    10561 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
    10621 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    10681 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    10741 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    10801 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
    10861 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
    10921 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
    10981 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
    11041 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    11101 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
    11161 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
    11221 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    11281 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    11341 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    11401 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    11461 tggcgtt
//
