LOCUS       sequence-229830-       11470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Pavicic M, Mouhu K, Wang F, Bilicka M, Chovancek E, Himanen K
  TITLE     Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin 
            E3 Ligases in Arabidopsis.
  JOURNAL   Front Plant Sci. 2017 Mar 28;8:416. doi: 10.3389/fpls.2017.00416. 
            eCollection 2017.
  PUBMED    28400782
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-03-28"; volume: "8:416. doi"; pages: " 
            10.3389/fpls.2017.00416. eCollection 2017"
COMMENT     Sequence Label: pBA cMYC-BPC4
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(835..1626)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2147..2171
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     2371..2393
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2385..2402
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2386..2402
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2462..3103)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(3147..3698)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(3749..3928)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4838..5183
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5126..5146
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    5216..5240
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6165..6189)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6221..6250
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6260..6289
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6299..6328
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6338..6367
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6401..6430
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      6514..6766
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6783..6799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6783..6799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6796..6818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6807..6823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6831..6837,6838..6855,6856..6861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6876..6897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7055..7079
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8410..9039
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9468..10541
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10607..10801
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11037..11059)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11145..11285
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11200..11219
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11300..11317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgcatgat atatctccca atttgtgtag ggcttattat gcacgcttaa aaataataaa
      721 agcagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca tctaacgctt
      781 gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta gacattattt
      841 gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc
      901 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac
      961 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc
     1021 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc
     1081 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa
     1141 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac
     1201 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc
     1261 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc
     1321 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag
     1381 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg
     1441 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt
     1501 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg
     1561 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc
     1621 ccccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
     1681 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     1741 agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
     1801 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta
     1861 cagcttacga accgaacagg cttatgtcca ctgggttcgt gcccgaattg atcacaggca
     1921 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     1981 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     2041 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     2101 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     2161 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
     2221 actgaattaa cgccgaattg ctctagcatt cgccattcag gctgcgcaac tgttgggaag
     2281 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     2341 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2401 gtgccaagct tgcatgcctg caggtcgact ctagtgggta ccagcttggg ctgcaggtcg
     2461 attgatgcat gttgtcaatc aattggcaag tcataaaatg cattaaaaaa tattttcata
     2521 ctcaactaca aatccatgag tataactata attataaagc aatgattaga atctgacaag
     2581 gattctggaa aattacataa aggaaagttc ataaatgtct aaaacacaag aggacatact
     2641 tgtattcagt aacatttgca gcttttctag gtctgaaaat atatttgttg cctagtgaat
     2701 aagcataatg gtacaactac aagtgtttta ctcctcatat taacttcggt cattagaggc
     2761 cacgatttga cacattttta ctcaaaacaa aatgtttgca tatctcttat aatttcaaat
     2821 tcaacacaca acaaataaga gaaaaaacaa ataatattaa tttgagaatg aacaaaagga
     2881 ccatatcatt cattaactct tctccatcca tttccatttc acagttcgat agcgaaaacc
     2941 gaataaaaaa cacagtaaat tacaagcaca acaaatggta caagaaaaac agttttccca
     3001 atgccataat actcaaactc agtaggattc tggtgtgtgc gcaatgaaac tgatgcattg
     3061 aacttgacga acgttgtcga aaccgatgat acgaacgaaa gctctagcta gaggatcagg
     3121 cctgaattca gatcctctag gggtcatcag atctcggtga cgggcaggac cggacggggc
     3181 ggtaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
     3241 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
     3301 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
     3361 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag
     3421 acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg
     3481 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
     3541 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
     3601 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
     3661 gcacggcgga tgtcggccgg gcgtcgttct gggctcatgg tagatccccc tagagtcgag
     3721 atctggattg agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt
     3781 cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg
     3841 aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg
     3901 ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg
     3961 cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat cttgatcccc
     4021 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
     4081 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
     4141 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
     4201 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
     4261 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc
     4321 agcgtgaagc ttgcatgcct gcaggtcccc agattagcct tttcaatttc agaaagaatg
     4381 ctaacccaca gatggttaga gaggcttacg cagcaggtct catcaagacg atctacccga
     4441 gcaataatct ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     4501 tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta
     4561 ttccagtatg gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag
     4621 tctctaaaaa ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg
     4681 acctaacaga actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca
     4741 atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa
     4801 atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa
     4861 tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag
     4921 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg
     4981 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     5041 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     5101 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     5161 gttcatttca tttggagaga acacggggga ctctagagga tctcgaggcg cgcccacaag
     5221 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atggagaatg gtggtcagta
     5281 tgataatgcg cggttcaagc ccgattactt caaaggtgca caatctatgt ggaatatgat
     5341 tccccagcat cagattaagg aacaacataa tgcgttggtg atgaataaga agatcatgtc
     5401 catccttgct gagagagacg ctgctgtaca tgaaagaaat caagctgtct ctgctaaaaa
     5461 ggaagctgtc gctgctcggg acgaagcgct tcagcaacgt gacaaagctc tttctgagag
     5521 agataaagct ttgatagaga gagataatgc ttatgctgct cttcaacacc atgaaaatag
     5581 tctcaacttt gctttgtctg gtgggaagtg tgttgatgga gatgattgtt ttggtattgg
     5641 ggaaccccac aaacttgaag tcttcccttt gtcaactatt ccgcctgagg ttacaaacac
     5701 gaaggtggtg aacaaaagga agaaagagaa caagcaggga ctatcaaagg tgaaaaaagt
     5761 gggtgaagat ctgaaccgtc gagttcctgc tcctggaaag aaatctagaa cagattggga
     5821 cagtcaagat gtcggtttga acttagtcac cttcgatgag acgacaatgc ctgtacccat
     5881 gtgttcgtgc acaggctcta cgcgtcagtg ttacaaatgg ggaaatggcg gctggcaatc
     5941 gtcttgctgc acaaccacct tatcccagta tccattaccg cagatgccaa acaagaggca
     6001 ttctcggatg ggcggtagga aaatgagcgg aaacgttttc tcgagattac tcagccgttt
     6061 atcagcagaa ggatatgatc tctcgtgtcc tgttgatctt aaagactatt gggctaggca
     6121 cgggacaaac cgctacatca ctatcaagaa gggtgggcgc gccgacccag ctttcttgta
     6181 caaagtggtg acgtctcgac ggtatcgatt caaagctatg gagcaaaagc tcatttctga
     6241 agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga
     6301 gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca tttctgaaga
     6361 ggacttgaat gaaatggaga gcttgggcga cctcaccatg gagcaaaagc tcatttctga
     6421 agaggacttg aattcggtac cccgggttcg aaatcgatgg atcctacgta gcctaggacg
     6481 cgtcttaatt aactagtgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     6541 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     6601 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     6661 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     6721 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attcgtaatc
     6781 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6841 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6901 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6961 atcggccaac gcgcggggag aggcggtttg cgtattggag cttgagcttg gatcagattg
     7021 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     7081 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     7141 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     7201 taaagtactt taaagtactt taaagtactt tgatccaacc cctccgctgc tatagtgcag
     7261 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     7321 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     7381 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     7441 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     7501 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     7561 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     7621 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     7681 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     7741 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     7801 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     7861 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     7921 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     7981 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     8041 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     8101 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     8161 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     8221 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     8281 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     8341 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     8401 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     8461 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     8521 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     8581 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     8641 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     8701 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     8761 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     8821 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     8881 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     8941 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     9001 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     9061 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     9121 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     9181 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     9241 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     9301 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     9361 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
     9421 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     9481 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     9541 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     9601 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     9661 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     9721 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     9781 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     9841 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     9901 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     9961 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
    10021 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
    10081 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
    10141 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
    10201 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
    10261 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
    10321 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
    10381 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
    10441 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    10501 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    10561 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
    10621 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    10681 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    10741 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    10801 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    10861 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    10921 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    10981 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    11041 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    11101 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    11161 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    11221 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    11281 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    11341 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    11401 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    11461 tgctggcgtt
//
