LOCUS       sequence-229837-       12341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Pavicic M, Mouhu K, Wang F, Bilicka M, Chovancek E, Himanen K
  TITLE     Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin 
            E3 Ligases in Arabidopsis.
  JOURNAL   Front Plant Sci. 2017 Mar 28;8:416. doi: 10.3389/fpls.2017.00416. 
            eCollection 2017.
  PUBMED    28400782
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-03-28"; volume: "8:416. doi"; pages: " 
            10.3389/fpls.2017.00416. eCollection 2017"
COMMENT     Sequence Label: pER8 cHA-FG1
FEATURES             Location/Qualifiers
     source          1..12341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             291..542
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             558..791
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             801..1736
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             849..1742
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        2300..2479
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2332..2515
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      3630..3882
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4203..4249
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    4289..4313
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4863..4887)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4898..4924
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4904..4930
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4934..4960
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4940..4966
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4970..4996
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4976..5002
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(5542..5559)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5542..5558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5551..5573)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5780..5804
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6325..7116
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7362..7950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7851..7870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8104..8121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8136..8276
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8202..8221)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8362..8384
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8620..8814
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8880..9953)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10382..11011)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
      301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
      361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
      421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
      481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
      541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
      721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
      781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
      841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
      901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
      961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     1141 tggagcaccc agggaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     1741 gagagctccc tggcggaatt cccagagatg ttagctgaaa tcatcactaa tcagatacca
     1801 aaatattcaa atggaaatat caaaaagctt ctgtttcatc aaaaatgact cgacctaact
     1861 gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     1921 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     1981 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     2041 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     2101 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     2161 agtaaaacac tagatcccca aacaagcttg aaactgaagg cgggaaacga caatctgatc
     2221 atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
     2281 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
     2341 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
     2401 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
     2461 tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga
     2521 tccgctagag gatctcgacc tgcaagatcc cggggggcaa tgagatatga aaaagcctga
     2581 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     2641 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     2701 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     2761 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     2821 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     2881 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     2941 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3001 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3061 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3121 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3181 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3241 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3301 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     3361 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     3421 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     3481 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     3541 cgccgatagt ggaaaccgac gccccagcac tcgtccggga tcttggaggt gatgtaacat
     3601 gatcacaagc tgatcccccg aatttccccg atcgttcaaa catttggcaa taaagtttct
     3661 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3721 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3781 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3841 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg atcccccctc
     3901 gacagcttgc atgccgcttg ggctgcaggt cgaggctaaa aaactaatcg cattatcatc
     3961 ccctcgacgt actgtacata taaccactgg ttttatatac agcagtactg tacatataac
     4021 cactggtttt atatacagca gtcgacgtac tgtacatata accactggtt ttatatacag
     4081 cagtactgta catataacca ctggttttat atacagcagt cgaggtaaga ttagatatgg
     4141 atatgtatat ggatatgtat atggtggtaa tgccatgtaa tatgctcgac tctaggatct
     4201 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaagc
     4261 tagtcgactc tagcctcgag gcgcgcccac aagtttgtac aaaaaagcag gctctatggc
     4321 ttctcgggtg gtgaggacta gtatgagact tcgaaggagg gatggtgtga ctcaaaacga
     4381 acaccaggga gatcaacaag tggctgacca agctccaacg gtgcctcaga ttgtggctac
     4441 tcctcctagg atcaatgtgg ttgctattga agatgatgat gatgttgttg aatcaactgc
     4501 ttctgctttt gctcaggcta aaaacaaatc gagaagtgca cgcagaggat cacaagtagt
     4561 tgatgtggag tcagctggtg ctaatagatc cacaaggcgt cgctctgatc aaactagtgt
     4621 tgactcagtt gagcttaaca agccgagaaa gtccaaggct gtggctcctc ctgtagagga
     4681 accaaaattt agctgtccaa tctgtctgtg tccatttact caggaggttt caacgaaatg
     4741 tggtcatatt ttctgcaaaa agtgcataaa gaatgcgtta tccctccaag ctaaatgccc
     4801 gacatgtagg aaaaaaatca ctgttaagga ccttatccgg gtcttccttc caaccaccag
     4861 aaacccagct ttcttgtaca aagtggttac gtacatgtat ccttatgatg ttccagatta
     4921 tgctagtctt atgtacccat acgacgtgcc tgactacgca tcattgatgt atccttatga
     4981 tgttccagat tatgctagtc tttaagggcc taggacgcgt cttaattaac tagtcgatcc
     5041 aggcctccca gctttcgtcc gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat
     5101 cagtttcatt gcccacacac cagaatccta ctaagtttga gtattatggc attggaaaag
     5161 ctgttttctt ctatcatttg ttctgcttgt aatttactgt gttctttcag tttttgtttt
     5221 cggacatcaa aatgcaaatg gatggataag agttaataaa tgatatggtc cttttgttca
     5281 ttctcaaatt attattatct gttgttttta ctttaatggg ttgaatttaa gtaagaaagg
     5341 aactaacagt gtgatattaa ggtgcaatgt tagacatata aaacagtctt tcacctctct
     5401 ttggttatgt cttgaattgg tttgtttctt cacttatctg tgtaatcaag tttactatga
     5461 gtctatgatc aagtaattat gcaatcaagt taagtacagt ataggctttt tgtgtcgagg
     5521 gggtaccgag tcgaggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5581 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5641 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgggtaccg
     5701 agctcgaatt caattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
     5761 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     5821 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     5881 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     5941 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     6001 atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag
     6061 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     6121 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     6181 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     6241 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     6301 cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa
     6361 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     6421 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     6481 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     6541 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     6601 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     6661 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     6721 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     6781 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     6841 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     6901 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     6961 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     7021 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     7081 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag
     7141 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc
     7201 tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc
     7261 ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc
     7321 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7381 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7441 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7501 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7561 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7621 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7681 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7741 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7801 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7861 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7921 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7981 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8041 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8101 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     8161 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     8221 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     8281 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8341 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8401 cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg
     8461 gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg
     8521 gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc
     8581 gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt
     8641 ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt
     8701 atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac
     8761 gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag
     8821 agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat
     8881 taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg
     8941 ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc
     9001 ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc
     9061 gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga
     9121 aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg
     9181 ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc
     9241 ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat
     9301 accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg
     9361 tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg
     9421 cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc
     9481 ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg
     9541 taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga
     9601 agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc
     9661 acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa
     9721 tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt
     9781 tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct
     9841 tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca
     9901 tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt
     9961 cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc
    10021 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
    10081 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
    10141 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
    10201 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
    10261 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
    10321 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
    10381 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
    10441 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
    10501 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
    10561 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
    10621 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
    10681 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
    10741 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
    10801 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
    10861 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
    10921 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
    10981 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
    11041 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
    11101 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
    11161 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
    11221 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
    11281 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
    11341 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
    11401 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
    11461 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
    11521 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
    11581 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
    11641 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
    11701 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
    11761 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
    11821 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
    11881 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
    11941 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
    12001 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
    12061 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
    12121 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
    12181 ggttggatca aagtacttta aagtacttta aagtacttta aagtactttg atcccgaggg
    12241 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
    12301 ttaaatatcc gattattcta ataaacgctc ttttctctta g
//
