LOCUS       sequence-229846-       12725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12725)
  AUTHORS   Pavicic M, Mouhu K, Wang F, Bilicka M, Chovancek E, Himanen K
  TITLE     Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin 
            E3 Ligases in Arabidopsis.
  JOURNAL   Front Plant Sci. 2017 Mar 28;8:416. doi: 10.3389/fpls.2017.00416. 
            eCollection 2017.
  PUBMED    28400782
REFERENCE   2  (bases 1 to 12725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-03-28"; volume: "8:416. doi"; pages: " 
            10.3389/fpls.2017.00416. eCollection 2017"
COMMENT     Sequence Label: pER8 cHA-AIP2
FEATURES             Location/Qualifiers
     source          1..12725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             291..542
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             558..791
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             801..1736
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             849..1742
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        2300..2479
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2332..2515
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      3630..3882
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4203..4249
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    4289..4313
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5247..5271)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5282..5308
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5288..5314
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5318..5344
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5324..5350
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5354..5380
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5360..5386
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(5926..5943)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5926..5942)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5935..5957)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6164..6188
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6709..7500
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7746..8334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8235..8254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8488..8505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8520..8660
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8586..8605)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8746..8768
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9004..9198
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9264..10337)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10766..11395)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
      301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
      361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
      421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
      481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
      541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
      721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
      781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
      841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
      901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
      961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     1141 tggagcaccc agggaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     1741 gagagctccc tggcggaatt cccagagatg ttagctgaaa tcatcactaa tcagatacca
     1801 aaatattcaa atggaaatat caaaaagctt ctgtttcatc aaaaatgact cgacctaact
     1861 gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     1921 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     1981 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     2041 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     2101 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     2161 agtaaaacac tagatcccca aacaagcttg aaactgaagg cgggaaacga caatctgatc
     2221 atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
     2281 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
     2341 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
     2401 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
     2461 tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga
     2521 tccgctagag gatctcgacc tgcaagatcc cggggggcaa tgagatatga aaaagcctga
     2581 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     2641 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     2701 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     2761 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     2821 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     2881 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     2941 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3001 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3061 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3121 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3181 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3241 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3301 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     3361 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     3421 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     3481 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     3541 cgccgatagt ggaaaccgac gccccagcac tcgtccggga tcttggaggt gatgtaacat
     3601 gatcacaagc tgatcccccg aatttccccg atcgttcaaa catttggcaa taaagtttct
     3661 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3721 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3781 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3841 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg atcccccctc
     3901 gacagcttgc atgccgcttg ggctgcaggt cgaggctaaa aaactaatcg cattatcatc
     3961 ccctcgacgt actgtacata taaccactgg ttttatatac agcagtactg tacatataac
     4021 cactggtttt atatacagca gtcgacgtac tgtacatata accactggtt ttatatacag
     4081 cagtactgta catataacca ctggttttat atacagcagt cgaggtaaga ttagatatgg
     4141 atatgtatat ggatatgtat atggtggtaa tgccatgtaa tatgctcgac tctaggatct
     4201 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaagc
     4261 tagtcgactc tagcctcgag gcgcgcccac aagtttgtac aaaaaagcag gctctatgga
     4321 tgcatcgtct tcaccgtctc cttccgagga aagcttgaag cttgagcttg atgatcttca
     4381 gaaacagctg aacaaaaagc tgagattcga agcatccgtt tgttctattc ataatcttct
     4441 ccgtgatcac tactcttctt cctctccttc tctccgcaaa cagttctata tagttgtatc
     4501 tcgtgtcgct acggttctta agacaagata tacagctact ggattttggg ttgctggact
     4561 gagtcttttc gaagaggctg agcgacttgt ctctgatgct tctgagaaga aacatttgaa
     4621 atcttgcgtt gctcaagcta aggagcagtt aagcgaagta gataatcagc caacagagag
     4681 ctcacaaggt tatctttttg agggacatct tacggttgat cgtgagccgc cacagcctca
     4741 gtggctagta cagcagaatc tcatgtctgc tttcgcttct atcgttggtg gtgaatcctc
     4801 taatggtcct actgaaaaca ctattgggga aactgctaac ttgatgcaag aacttatcaa
     4861 tggtcttgac atgatcattc cagatatact agatgatggt ggaccaccaa gagctccacc
     4921 ggcaagtaaa gaagttgtag agaaactccc agtcattatt ttcaccgagg aattgcttaa
     4981 aaagtttgga gcagaggcag aatgttgcat ctgcaaggag aatctagtta ttggcgacaa
     5041 gatgcaggaa ttgccatgca agcacacatt tcaccctcct tgcctaaagc cttggctgga
     5101 cgagcataac tcttgcccta tatgccgcca tgaattacca acagacgatc agaaatacga
     5161 aaactggaaa gagagagaga aagaggccga agaagagagg aagggcgcag agaatgctgt
     5221 ccgcggaggt gaatatatgt acgttaaccc agctttcttg tacaaagtgg ttacgtacat
     5281 gtatccttat gatgttccag attatgctag tcttatgtac ccatacgacg tgcctgacta
     5341 cgcatcattg atgtatcctt atgatgttcc agattatgct agtctttaag ggcctaggac
     5401 gcgtcttaat taactagtcg atccaggcct cccagctttc gtccgtatca tcggtttcga
     5461 caacgttcgt caagttcaat gcatcagttt cattgcccac acaccagaat cctactaagt
     5521 ttgagtatta tggcattgga aaagctgttt tcttctatca tttgttctgc ttgtaattta
     5581 ctgtgttctt tcagtttttg ttttcggaca tcaaaatgca aatggatgga taagagttaa
     5641 taaatgatat ggtccttttg ttcattctca aattattatt atctgttgtt tttactttaa
     5701 tgggttgaat ttaagtaaga aaggaactaa cagtgtgata ttaaggtgca atgttagaca
     5761 tataaaacag tctttcacct ctctttggtt atgtcttgaa ttggtttgtt tcttcactta
     5821 tctgtgtaat caagtttact atgagtctat gatcaagtaa ttatgcaatc aagttaagta
     5881 cagtataggc tttttgtgtc gagggggtac cgagtcgagg aattcactgg ccgtcgtttt
     5941 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6001 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6061 gcgcagcctg aatggcgggt accgagctcg aattcaattc ggcgttaatt cagtacatta
     6121 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     6181 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     6241 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     6301 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     6361 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     6421 tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     6481 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     6541 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     6601 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     6661 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
     6721 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     6781 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     6841 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     6901 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     6961 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     7021 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     7081 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     7141 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     7201 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     7261 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     7321 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     7381 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     7441 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     7501 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     7561 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     7621 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
     7681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8521 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     8581 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     8641 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     8701 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     8761 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
     8821 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
     8881 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
     8941 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
     9001 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
     9061 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
     9121 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
     9181 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
     9241 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
     9301 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
     9361 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
     9421 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
     9481 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
     9541 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
     9601 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
     9661 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
     9721 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
     9781 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
     9841 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
     9901 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
     9961 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
    10021 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
    10081 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
    10141 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
    10201 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
    10261 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
    10321 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
    10381 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
    10441 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
    10501 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
    10561 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
    10621 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
    10681 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
    10741 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
    10801 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
    10861 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
    10921 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
    10981 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
    11041 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
    11101 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
    11161 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
    11221 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
    11281 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
    11341 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
    11401 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
    11461 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
    11521 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
    11581 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
    11641 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
    11701 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
    11761 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
    11821 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
    11881 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
    11941 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
    12001 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
    12061 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
    12121 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    12181 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    12241 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    12301 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    12361 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    12421 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    12481 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    12541 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac tttaaagtac
    12601 tttaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga
    12661 acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct
    12721 cttag
//
